ABIF etdir @ 2015-11-24 04:00:00.0 2013-06-08 09:12:00.0 1503232 WSBS Custom BD 3.1 POP7WSBS Custom BD 3.1 POP7 KB 1.4.1 525.0KB_3500_POP7_BDTv3.mobKB.bcpfalse100falsetruefalsetrue20true10001.0800true0.030,15300,100300, 1008,44truetrue4truetruetrue205504true20false20202020falsetrue42020false & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & & SequencingPOP7508// FastSeq50POP7 module edited 09/04/08
// Standard Sequencing module -- 8 capillary
//
//REGULARDC_RunModule_Comment;FastSeq50POP7 module edited 09/04/08;List.;STRING;nk;nk;Run Module Comment;falseOven_Temperature;60;Deg. C;INTEGER;18;65;Oven Temperature throughout the run;trueTemperature_Tolerance;3;Deg. C;INTEGER;0;50;Tolerance of the temperature that the system will use during its wait for temperature state;falseOven_Wait_Time;1800;sec.;INTEGER;1;1800;Timeout for oven wait for temperature;falseArray_Heater_Temperature;50;Deg. C;FLOAT;30.0;50.0;Detection cell heater temperature setting;falsePoly_Fill_Vol;2000;steps;INTEGER;1000;4600;We recommend that you do not change this value unless advised otherwise by Applied Biosystems support personnel;trueCap_Fill_Vol;1195;steps;INTEGER;1;4600;Number of steps to pump during capillary fill;falseDC_Cap8_EPS_ARC_SELF;4;uAmps;INTEGER;1;1000;Arc current self test for 8 cap array;falseMax_Current;425;uAmps;INTEGER;1;500;Power supply current limit;falseCurrent_Stability;20.1;uAmps;FLOAT;0;1000;Max Current Variance for EP;trueVoltage_Tolerance;0.6;kVolts;FLOAT;0.0;5.0;Power supply voltage tolerance;falsePreRun_Voltage;15;kVolts;FLOAT;0;18;Pre-Run Voltage;trueDC_PreRun_Time;180;sec.;INTEGER;1;1000;Length of Pre-Run;trueInjection_Voltage;1.6;kVolts;FLOAT;1;15;Injection Voltage;trueDC_Injection_Time;8;sec.;INTEGER;1;600;Injection Time;trueInjection_Depth_Offset;0;steps;INTEGER;-500;500;Injection Depth Offset;falseVoltage_Number_Of_Steps;30;nk;INTEGER;1;100;We recommend that you do not change this value unless advised otherwise by Applied Biosystems support personnel;trueVoltage_Step_Interval;15;sec;INTEGER;1;60;We recommend that you do not change this value unless advised otherwise by Applied Biosystems support personnel;trueVoltage_Ramp_Wait;450;sec;INTEGER;1;1000;We recommend that you do not change this value unless advised otherwise by Applied Biosystems support personnel;falseDC_Data_Delay;250;sec.;INTEGER;1;3600;Time delay before beginning data collection;trueRun_Voltage;13.4;kVolts;FLOAT;0;19.5;Separation (run) Voltage.;trueDC_Run_Time;2520;sec.;INTEGER;300;14000;Separation (run) Time;trueFirst_ReadOut_Time;200;msec.;INTEGER;100;5000;Sampling rate in milliseconds coming from the camera;falseSecond_ReadOut_Time;200;msec.;INTEGER;100;5000;Sampling rate in milliseconds coming from the camera;falseExposure_Time;0;msec.;INTEGER;0;5000;CCD exposure time = integration time in milliseconds;falseDC_RS_Plate_Type;$DC_RS_Plate_Type;List.;STRING;nk;nk;Plate type indicator;falseDC_RS_CSData;$DC_RS_CSData;List;STRING;nk;nk;Capillary mapping;falseDC_RS_Binning_Pattern;;pixels;STRING;;;spectral binning pattern;falseDC_Scale_Divisor;2.0;;FLOAT;;;scale divisor;falseDC_Down_Sample;1;;INTEGER;;;number of frames to average;falseDC_Laser_Power_Setting;15.10;mWatts;FLOAT;;;laser power setting;false//
//
SYSTem:MESSage "$DC_RunModule_Comment"
//
//
//
// Turning Laser On
//
SYSTem:MESSage "Turning Laser On."
LAS:POW:SETT $DC_Laser_Power_Setting
LAS:POW:TOL 1.0
LAS On
//
//
// Turning Zohana On
//
SYSTem:MESSage "Turning Detection Heater On."
DETH:TEMP:TOL 3.0
DETH:TEMP:SETT $Array_Heater_Temperature
DETH On
//
//
// Turning Oven On
//
SYSTem:MESSage "Setting Oven Temperature."
OVEN:TEMP:TOL $Temperature_Tolerance
OVEN:TEMP:SETT $Oven_Temperature
OVEN On
//
//
// Initializing Autosampler
//
SYSTem:MESSage "Initializing Autosampler."
STR Initialize
//
// Filling Polymer Pump
//
SYSTem:MESSage "Loading Waste Tray."
STR:ROB:MOV:LOAD Waste
FLU:POLY:PRES Up 0.34 50
FLU:POLY:PRES Down 0.21 200
SYSTem:MESSage "Filling Polymer Pump"
FLU:POLY:FILL $Poly_Fill_Vol
//
// Waiting for Oven to Stabilize at Run Temperature
//
SYSTem:MESSage "Waiting for Oven to Stabilize at Run Temperature"
OVEN:SYNC $Oven_Wait_Time
//
// Waiting for Detection Heater to Stabilize at Run Temperature
//
SYSTem:MESSage "Waiting for Detection Heater to Stabilize at Run Temperature"
DETH:SYNC 1800
//
// Waiting for Laser to Stabilize at Power Setting
//
SYSTem:MESSage "Waiting for Laser to Stabilize at Power Setting"
LAS:SYNC 600
//
//
// Filling Array
//
SYSTem:MESSage "Filling Array with Polymer."
FLU:CAP:FILL:THRE 1000
FLU:CAP:FILL $Cap_Fill_Vol
//
// Rinsing Capillaries
//
SYSTem:MESSage "Rinsing Capillary Tips."
STR:ROB:MOV:LOAD Water
SYST:WAIT 3
//
SYSTem:MESSage "Loading Buffer Tray."
STR:ROB:MOV:LOAD Buffer
SYST:WAIT 3
//
// Bubble Check
//
SYSTem:MESSage "Testing for Normal Current."
EPS:CURR:LIM $Max_Current
EPS:CURR:ARC:THRE $Current_Stability
EPS:VOLT:SETT 2.0
EPS On
SYST:WAIT 2
EPS:CURR:ARC:SELF $DC_Cap8_EPS_ARC_SELF
EPS Off
//
// Pre Run
//
SYSTem:MESSage "PreRun"
EPS:VOLT:TOL $Voltage_Tolerance
EPS:VOLT:SETT $PreRun_Voltage
EPS On
EPS:SYNC 10
SYST:WAIT $DC_PreRun_Time
EPS Off
SYST:WAIT 3
//
//
// Rinse Capillaries
//
SYSTem:MESSage "Rinsing Capillary Tips."
STR:ROB:MOV:LOAD Water
SYST:WAIT 3
//
// Sample injection
//
SYSTem:MESSage "Sample Injection."
STR:ROB:ZOFF $Injection_Depth_Offset
STR:CSO $DC_RS_CSData
STR:ROB:MOV:LOAD $DC_RS_Plate_Type
SYST:WAIT 15
EPS:VOLT:SETT $Injection_Voltage
EPS On
SYST:WAIT $DC_Injection_Time
EPS Off
EPS:VOLT:SETT 0.0
SYST:WAIT 1
STR:ROB:ZOFF 0
//
// Rinsing Capillaries
//
SYSTem:MESSage "Rinsing Capillary Tips."
STR:ROB:MOV:LOAD Water
SYST:WAIT 3
//
// Starting Electrophoresis
//
SYSTem:MESSage "Loading Buffer Tray."
STR:ROB:MOV:LOAD Buffer
SYST:WAIT 3
SYSTem:MESSage "Ramping Cycle."
EPS:VOLT:TOL $Voltage_Tolerance
EPS:VOLT:SQRR $Run_Voltage $Voltage_Number_Of_Steps $Voltage_Step_Interval
EPS On
SYST:WAIT $Voltage_Ramp_Wait
EPS:SYNC 10
//
// Data Delay
//
SYSTem:MESSage "Waiting for Data."
SYST:WAIT $DC_Data_Delay
//
// CCD beginning data read
//
CAM:SHUT Auto
DAT:SRAT $First_ReadOut_Time $Second_ReadOut_Time
DAT:MODe Binning
SYSTem:MESSage "Starting Data Collection."
DAT:COLL Start
SYST:WAIT $DC_Run_Time
SYSTem:MESSage "Stop Data Collection."
DAT:COLL Stop
EPS Off
EPS:VOLT:SETT 0.0
SYST:WAIT 1
//LAS Off
//DETH Off
//OVEN Off
//
//
// End Of Module4[0,1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20;0,0,12,24,36,48,60,72,84,96,108,120,132,144,156,168,180,192,204,216,228;0,11,23,35,47,59,71,83,95,107,119,131,143,155,167,179,191,203,215,227,239][100.0,200.0,300.0,400.0][100.0,200.0,300.0,400.0]3AppliedBiosystems1.05.50.950.99Applied Biosystems[Dye1;540;1, Dye2;568;2, Dye3;595;3, Dye4;615;4][POP4, POP6, POP7][36, 50][matrixStandard, sequenceStandard]525.0KB_3500_POP7_BDTv3.mobKB.bcpfalse100falsetruefalsetrue20true10001.0800true0.030,15300,100300, 1008,44truetrue4truetruetrue205504true20false20202020falsetrue42020WSBS ~700bp BD3.1 POP7 v1.0.0 2013-06-12 04:05:52 -07:002014-05-27 15:55:00.0 2011-01-20 23:14:00.0 L513F1205 2014-01-17 04:00:00.0 2013-06-08 09:12:00.0 1304140 pass WSBS-Seq-1-08-15 Comment:ev+EzZPKt5H7u9UzEwTYpg== e &
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n a M P : A 2 7 - 5 @ T \ l } r \ S = 8 2 & & " # ! & # # ' % , 0 , - $ ! " ' $ % % " , 5 8 K G F H ? D K ] } d I D ( 1 0 + 7 9 4 / 9 ? J q 4SY> } q s p i G A 7 + # + & ) ! ! "
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//
SYSTem:MESSage "$DC_RunModule_Comment"
//
//
//
// Turning Laser On
//
SYSTem:MESSage "Turning Laser On."
LAS:POW:SETT $DC_Laser_Power_Setting
LAS:POW:TOL 1.0
LAS On
//
//
// Turning Zohana On
//
SYSTem:MESSage "Turning Detection Heater On."
DETH:TEMP:TOL 3.0
DETH:TEMP:SETT $Array_Heater_Temperature
DETH On
//
//
// Turning Oven On
//
SYSTem:MESSage "Setting Oven Temperature."
OVEN:TEMP:TOL $Temperature_Tolerance
OVEN:TEMP:SETT $Oven_Temperature
OVEN On
//
//
// Initializing Autosampler
//
SYSTem:MESSage "Initializing Autosampler."
STR Initialize
//
// Filling Polymer Pump
//
SYSTem:MESSage "Loading Waste Tray."
STR:ROB:MOV:LOAD Waste
FLU:POLY:PRES Up 0.34 50
FLU:POLY:PRES Down 0.21 200
SYSTem:MESSage "Filling Polymer Pump"
FLU:POLY:FILL $Poly_Fill_Vol
//
// Waiting for Oven to Stabilize at Run Temperature
//
SYSTem:MESSage "Waiting for Oven to Stabilize at Run Temperature"
OVEN:SYNC $Oven_Wait_Time
//
// Waiting for Detection Heater to Stabilize at Run Temperature
//
SYSTem:MESSage "Waiting for Detection Heater to Stabilize at Run Temperature"
DETH:SYNC 1800
//
// Waiting for Laser to Stabilize at Power Setting
//
SYSTem:MESSage "Waiting for Laser to Stabilize at Power Setting"
LAS:SYNC 600
//
//
// Filling Array
//
SYSTem:MESSage "Filling Array with Polymer."
FLU:CAP:FILL:THRE 1000
FLU:CAP:FILL $Cap_Fill_Vol
//
// Rinsing Capillaries
//
SYSTem:MESSage "Rinsing Capillary Tips."
STR:ROB:MOV:LOAD Water
SYST:WAIT 3
//
SYSTem:MESSage "Loading Buffer Tray."
STR:ROB:MOV:LOAD Buffer
SYST:WAIT 3
//
// Bubble Check
//
SYSTem:MESSage "Testing for Normal Current."
EPS:CURR:LIM $Max_Current
EPS:CURR:ARC:THRE $Current_Stability
EPS:VOLT:SETT 2.0
EPS On
SYST:WAIT 2
EPS:CURR:ARC:SELF $DC_Cap8_EPS_ARC_SELF
EPS Off
//
// Pre Run
//
SYSTem:MESSage "PreRun"
EPS:VOLT:TOL $Voltage_Tolerance
EPS:VOLT:SETT $PreRun_Voltage
EPS On
EPS:SYNC 10
SYST:WAIT $DC_PreRun_Time
EPS Off
SYST:WAIT 3
//
//
// Rinse Capillaries
//
SYSTem:MESSage "Rinsing Capillary Tips."
STR:ROB:MOV:LOAD Water
SYST:WAIT 3
//
// Sample injection
//
SYSTem:MESSage "Sample Injection."
STR:ROB:ZOFF $Injection_Depth_Offset
STR:CSO $DC_RS_CSData
STR:ROB:MOV:LOAD $DC_RS_Plate_Type
SYST:WAIT 15
EPS:VOLT:SETT $Injection_Voltage
EPS On
SYST:WAIT $DC_Injection_Time
EPS Off
EPS:VOLT:SETT 0.0
SYST:WAIT 1
STR:ROB:ZOFF 0
//
// Rinsing Capillaries
//
SYSTem:MESSage "Rinsing Capillary Tips."
STR:ROB:MOV:LOAD Water
SYST:WAIT 3
//
// Starting Electrophoresis
//
SYSTem:MESSage "Loading Buffer Tray."
STR:ROB:MOV:LOAD Buffer
SYST:WAIT 3
SYSTem:MESSage "Ramping Cycle."
EPS:VOLT:TOL $Voltage_Tolerance
EPS:VOLT:SQRR $Run_Voltage $Voltage_Number_Of_Steps $Voltage_Step_Interval
EPS On
SYST:WAIT $Voltage_Ramp_Wait
EPS:SYNC 10
//
// Data Delay
//
SYSTem:MESSage "Waiting for Data."
SYST:WAIT $DC_Data_Delay
//
// CCD beginning data read
//
CAM:SHUT Auto
DAT:SRAT $First_ReadOut_Time $Second_ReadOut_Time
DAT:MODe Binning
SYSTem:MESSage "Starting Data Collection."
DAT:COLL Start
SYST:WAIT $DC_Run_Time
SYSTem:MESSage "Stop Data Collection."
DAT:COLL Stop
EPS Off
EPS:VOLT:SETT 0.0
SYST:WAIT 1
//LAS Off
//DETH Off
//OVEN Off
//
//
// End Of ModuleFastSeq50_POP7_1 v1.0.0 Run 2013-06-11-22-36-52-078 S2015-09-14 05:00:00.02013-06-08 09:12:00.0 1501067KB.bcp
KB 1.4.1.8
6228001-05Sequencing Format 2.0Run 2011-01-22-01-10-50-980 UseClearRangeThreshold=true,UseLastBase=true,MaxLowQualityBases3PrimeEnd=4,UseFirstBase=true,UseClearRangeThreshold5PrimeEnd=true,LastBase=550,UseClearRangeThreshold3PrimeEnd=true,LowQualityWindow5PrimeEnd=20,UseAmpliconPrimerTrimming=false,LowQualityWindow3PrimeEnd=20,FirstBase=20,CvThreshold5PrimeEnd=20,CvThreshold3PrimeEnd=20,UseM13Masking=false,UseConfidenceRange=false,MaxLowQualityBases5PrimeEnd=4,BasesToTrimFrom3PrimeEnd=20 check termABED ABID ABLt ABRn ABTp ABC AEPt &: AEPt &: APFN APXV 1 APrN APrV APrX 3 3 ARTN ASPF ASPt B ASPt B AVld
AmbT
& AsyC 9m 9m AsyN Lc AsyV Lz B1Pt 1 B1Pt 1 BCTS L BcRn BcRs BcRs CAED L CAID L CALt 12 CARn r CASN
L CBED L CBID L CBLt L CBRn CBTp CBC CLRG CLRG [ CRLn i CRLn M CTNM M CTTL M CkSm M% CpEP DATA 1 b M> DATA 1 b P DATA 1 b b DATA 1 b st DATA Ն DATA V DATA & DATA DATA $ I DATA
$ I 6^ DATA $ I DATA $ I Ɏ DSam DySN Z Dye# DyeN & DyeN + DyeN 1 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt 4X EVNT = EVNT I EVNT U EVNT h FTab { FVoc FWO_ GATC Feat / / GTyp HCFG CE HCFG HCFG HCFG ? ? InSc InVt @ InjN " " LANE LAST > LIMS % % LNTD 2 LsrP : MCHN MODF MODL 3500 NAVG NLNE NOIS P1AM P1RL P1WD ") P2AM (5 P2BA .A P2RL 1G PBAS 7S PBAS :Y PCON =_ PCON @e PDMF Ck PDMF C PLOC C PLOC I PSZE ` PTYP O PXLB PuSc PuSc O QV20 = QV20 O QcPa O QcRn QcRs QcRs RGNm : : P RMXV P RMdN Q RMdV Q RMdX / / Q RNmF ? RPrN ]G RPrV ]X RUND
RUND
RUND
RUND
RUNT RUNT * RUNT RUNT Rate ]_ RunN ]k S/N% ] SCAN 1 SMED ] SMID ] SMLt ] SMPL Tih SMRn SPAC AAsL SPAC ] SPAC AAsL SVER 3.0 SVER ] SVER ] SVER ] ScSt Scal @ Scan 1 SpeN ] TUBE A05 Tmpr < TrPa ^ TrSc TrSc _ phAR phCH _ phDY big phQL c phTR phTR N3500 Data Collection Version 1.0 - 3500 FC_090519180136-0700 Build Number: 837ABED ABID ABLt ABRn ABTp ABC AEPt &: AEPt &: APFN APXV 1 APrN APrV APrX 3 3 ARTN ASPF ASPt B ASPt B AVld
AmbT
& AsyC 9m 9m AsyN Lc AsyV Lz B1Pt 1 B1Pt 1 BCTS L BcRn BcRs BcRs CAED L CAID L CALt 12 CARn r CASN
L CBED L CBID L CBLt L CBRn CBTp CBC CLRG CLRG [ CRLn i CRLn M CTNM M CTTL M CkSm M% CpEP DATA 1 b M> DATA 1 b P DATA 1 b b DATA 1 b st DATA Ն DATA V DATA & DATA DATA $ I DATA
$ I 6^ DATA $ I DATA $ I Ɏ DSam DySN Z Dye# DyeN & DyeN + DyeN 1 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt 4X EVNT = EVNT I EVNT U EVNT h FTab { FVoc FWO_ GATC Feat / / GTyp HCFG CE HCFG HCFG HCFG ? ? InSc InVt @ InjN " " LANE LAST > LIMS % % LNTD 2 LsrP : MCHN MODF MODL 3500 NAVG NLNE NOIS P1AM P1RL P1WD ") P2AM (5 P2BA .A P2RL 1G PBAS 7S PBAS :Y PCON =_ PCON @e PDMF Ck PDMF C PLOC C PLOC I PSZE ` PTYP O PXLB PuSc PuSc O QV20 = QV20 O QcPa O QcRn QcRs QcRs RGNm : : P RMXV P RMdN Q RMdV Q RMdX / / Q RNmF ? RPrN ]G RPrV ]X RUND
RUND
RUND
RUND
RUNT RUNT * RUNT RUNT Rate ]_ RunN ]k S/N% ] SCAN 1 SMED ] SMID ] SMLt ] SMPL Tih SMRn SPAC AAsL SPAC ] SPAC AAsL SVER O O s SVER ] SVER ] SVER ] ScSt Scal @ Scan 1 SpeN ] TUBE A05 Tmpr < TrPa ^ TrSc TrSc _ phAR phCH _ phDY big phQL c phTR phTR GIK21XfTIcvZVeF6tZGzZw== AAct
ABED ABID ABLt ABRn ABTp ABC AEPt &: AEPt &: APFN APXV 1 APrN APrV APrX 3 3 ARTN ASPF ASPt B ASPt B AVld
AmbT
& AsyC 9m 9m AsyN Lc AsyV Lz B1Pt 1 B1Pt 1 BCTS L BcRn BcRs BcRs CAED L CAID L CALt 12 CARn r CASN
L CBED L CBID L CBLt L CBRn CBTp CBC CLRG CLRG [ CRLn i CRLn M CTNM M CTTL M CkSm CpEP DATA 1 b M> DATA 1 b P DATA 1 b b DATA 1 b st DATA Ն DATA V DATA & DATA DATA $ I DATA
$ I 6^ DATA $ I DATA $ I Ɏ DSam DySN Z Dye# DyeN & DyeN + DyeN 1 DyeN 7 DyeW DyeW 8 DyeW S DyeW g EPVt 4X EVNT = EVNT I EVNT U EVNT h FTab { FVoc FWO_ GATC Feat / / GTyp HCFG CE HCFG HCFG HCFG ? ? InSc InVt @ InjN " " LANE LAST > LIMS % % LNTD 2 LsrP : MCHN MODF MODL 3500 NAVG NLNE NOIS P1AM P1RL P1WD ") P2AM (5 P2BA .A P2RL 1G PBAS 7S PBAS :Y PCON =_ PCON @e PDMF Ck PDMF C PLOC C PLOC I PSZE ` PTYP O PXLB PuSc PuSc O QV20 = QV20 O QcPa O QcRn QcRs QcRs RGNm : : P RMXV P RMdN Q RMdV Q RMdX / / Q RNmF ? RPrN ]G RPrV ]X RUND
RUND
RUND
RUND
RUNT RUNT * RUNT RUNT Rate ]_ RunN ]k S/N% ] SCAN 1 SMED ] SMID ] SMLt ] SMPL Tih SMRn SPAC AAsL SPAC ] SPAC AAsL SVER O O s SVER ] SVER ] SVER ] ScSt Scal @ Scan 1 SpeN ] TUBE A05 Tmpr < TrPa ^ TrSc TrSc _ phAR phCH _ phDY big phQL c phTR phTR