SUMMARISING RUN PARAMETERS
==========================
Input filename: SRR389222_sub3.fastq.gz
Trimming mode: single-end
Trim Galore version: 0.6.7
Cutadapt version: 3.4
Number of cores used for trimming: 1
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Unable to auto-detect most prominent adapter from the first specified file (count Illumina: 0, count Nextera: 0, count smallRNA: 0)
Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior).
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection))
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length before a sequence gets removed: 20 bp
Running FastQC on the data once trimming has completed
Output file will be GZIP compressed


This is cutadapt 3.4 with Python 3.7.6
Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR389222_sub3.fastq.gz
Processing reads on 1 core in single-end mode ...
Finished in 1.57 s (20 µs/read; 3.06 M reads/minute).

=== Summary ===

Total reads processed:                  80,000
Reads with adapters:                    28,986 (36.2%)
Reads written (passing filters):        80,000 (100.0%)

Total basepairs processed:     2,880,000 bp
Quality-trimmed:                  17,739 bp (0.6%)
Total written (filtered):      2,827,473 bp (98.2%)

=== Adapter 1 ===

Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 28986 times

No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1

Bases preceding removed adapters:
  A: 27.0%
  C: 34.3%
  G: 3.8%
  T: 34.9%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	25283	20000.0	0	25283
2	2467	5000.0	0	2467
3	688	1250.0	0	688
4	403	312.5	0	403
5	62	78.1	0	62
6	35	19.5	0	35
7	27	4.9	0	27
8	8	1.2	0	8
9	9	0.3	0	9
10	3	0.1	1	3
11	1	0.0	1	1

RUN STATISTICS FOR INPUT FILE: SRR389222_sub3.fastq.gz
=============================================
80000 sequences processed in total
Sequences removed because they became shorter than the length cutoff of 20 bp:	123 (0.2%)