SUMMARISING RUN PARAMETERS
==========================
Input filename: SRR389222_sub2.fastq.gz
Trimming mode: single-end
Trim Galore version: 0.6.7
Cutadapt version: 3.4
Number of cores used for trimming: 1
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Unable to auto-detect most prominent adapter from the first specified file (count Nextera: 0, count smallRNA: 0, count Illumina: 0)
Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior).
Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection))
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length before a sequence gets removed: 20 bp
Running FastQC on the data once trimming has completed
Output file will be GZIP compressed


This is cutadapt 3.4 with Python 3.7.6
Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC SRR389222_sub2.fastq.gz
Processing reads on 1 core in single-end mode ...
Finished in 2.61 s (22 µs/read; 2.75 M reads/minute).

=== Summary ===

Total reads processed:                 120,000
Reads with adapters:                    43,714 (36.4%)
Reads written (passing filters):       120,000 (100.0%)

Total basepairs processed:     4,320,000 bp
Quality-trimmed:                  25,837 bp (0.6%)
Total written (filtered):      4,241,537 bp (98.2%)

=== Adapter 1 ===

Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 43714 times

No. of allowed errors:
1-9 bp: 0; 10-13 bp: 1

Bases preceding removed adapters:
  A: 26.6%
  C: 34.5%
  G: 4.0%
  T: 34.9%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	38000	30000.0	0	38000
2	3834	7500.0	0	3834
3	1048	1875.0	0	1048
4	595	468.8	0	595
5	103	117.2	0	103
6	65	29.3	0	65
7	41	7.3	0	41
8	18	1.8	0	18
9	5	0.5	0	5
10	2	0.1	1	2
11	3	0.0	1	3

RUN STATISTICS FOR INPUT FILE: SRR389222_sub2.fastq.gz
=============================================
120000 sequences processed in total
Sequences removed because they became shorter than the length cutoff of 20 bp:	172 (0.1%)