FastQCFastQC Report
Mon 13 Sep 2021
SRR389222_sub3_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389222_sub3_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79877
Sequences flagged as poor quality0
Sequence length20-36
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTAGGTTAGTTGTTTGTTAGTGTTGTTTTTTGG56397.059604141367353No Hit
GATTTGATAAATATAGATTGGATTTATGGGGTGGAT51146.402343603290059No Hit
AATTACCATCCACCTTATAACTACCACTATAAATC46755.852748600973998No Hit
TTACCAATAACTAACTAAACACACCATTAATAATCT46235.787648509583485No Hit
ATAAAACCTATCCAAACAATCTACATAACCAAACAC6000.7511549006597643No Hit
TACCAATAACTAACTAAACACACCATTAATAATCT4900.6134431688721409No Hit
CTATAAACCATCATAACACCAATAAAACCTATCCA4740.5934123715212138No Hit
GTTGTGTTGGAGAAATAGGGTGTGGAAATTATGTTG4180.5233045807929693No Hit
GAAATAGGGTGTGGAAATTATGTTGATTTATAGTGG3690.4619602639057551No Hit
GTTGGAGAAATAGGGTGTGGAAATTATGTTGATTT3560.44568524105812685No Hit
GTAATTATGGTGTTGTGTTGGAGAAATAGGGTGTGG3460.43316599271379747No Hit
GTTATGATGGTTTATAGTAATTATGGTGTTGTGTTG3370.42189866920390107No Hit
ATGATGAAAAGTTGTAGGTATAGTAGGTAGTGT3300.4131351953628704No Hit
CTCACAATCAAAATTAACCATCTATACAACAATATT3300.4131351953628704No Hit
GAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTATT3240.4056236463562728No Hit
ATACAACAATATTTTTCATAAATCCACCCCATAAAT3170.3968601725152422No Hit
GTATGATGAAAAGTTGTAGGTATAGTAGGTAGTGT3110.3893486235086445No Hit
TGGGGTGGATTTATGAAAAATATTGTTGTATAGATG2990.37432552549544923No Hit
CATAACCAAACACCAAAAAACAACACTAACAAACA2950.36931782615771747No Hit
CAAACAATCTACATAACCAAACACCAAAAAACAAC2920.3655620516544187No Hit
ATCCAAACAATCTACATAACCAAACACCAAAAAAC2820.35304280331008925No Hit
ATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGAA2780.34803510397235754No Hit
ATAATATGTTGGAATAGTATGATGAAAAGTTGTAGG2780.34803510397235754No Hit
ATTACCATCCACCTTATAACTACCACTATAAATCA2710.3392716301313269No Hit
CTACATAACCAAACACCAAAAAACAACACTAACAA2670.3342639307935952No Hit
CTATCCAAACAATCTACATAACCAAACACCAAAAA2620.32800430662143043No Hit
AAACAATCTACATAACCAAACACCAAAAAACAACAC2580.32299660728369867No Hit
ATCATAACACCAATAAAACCTATCCAAACAATCTAC2530.316736983111534No Hit
CATCTATACAACAATATTTTTCATAAATCCACCCC2510.3142331334426681No Hit
GGTATAGTAGGTAGTGTAGATTATTAATGGTGTGTT2460.3079735092705034No Hit
GTTGGATTGTTAATAATATGTTGGAATAGTATGATG2410.3017138850983387No Hit
ATCAAAATTAACCATCTATACAACAATATTTTTCAT2360.295454260926174No Hit
ATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT2310.2891946367540093No Hit
ATAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGT2240.2804311629129787No Hit
TGAAAAATATTGTTGTATAGATGGTTAATTTTGATT2230.2791792380785458No Hit
ATTTTGATTGTGAGTAGATGTGTTGGATTGTTAAT2230.2791792380785458No Hit
TAGATTGGATTTATGGGGTGGATTTATGAAAAATAT2210.27667538840967987No Hit
ATAAACCATCATAACACCAATAAAACCTATCCAAAC2200.27542346357524694No Hit
TTAACAATCCAACACATCTACTCACAATCAAAATT2200.27542346357524694No Hit
GTGGAAATTATGTTGATTTATAGTGGTAGTTATA2180.27291961390638103No Hit
TAATAATATGTTGGAATAGTATGATGAAAAGTTGT2150.26916383940308225No Hit
AATAAAACCTATCCAAACAATCTACATAACCAAAC2100.26290421523091756No Hit
CAACAATATTTTTCATAAATCCACCCCATAAATCC2040.2553926662243199No Hit
CTACACTACCTACTATACCTACAACTTTTCATCAT2000.2503849668865881No Hit
CTATACAACAATATTTTTCATAAATCCACCCCATA1990.2491330420521552No Hit
ATATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT1970.24662919238328931No Hit
CATAATTTCCACACCCTATTTCTCCAACACAACACC1930.24162149304555752No Hit
ATCTACATAACCAAACACCAAAAAACAACACTAAC1920.2403695682111246No Hit
CATAACACCAATAAAACCTATCCAAACAATCTACAT1900.23786571854225871No Hit
TTGTGAGTAGATGTGTTGGATTGTTAATAATATGTT1880.23536186887339283No Hit
ATAATTACTATAAACCATCATAACACCAATAAAACC1870.2341099440389599No Hit
GAAAAATATTGTTGTATAGATGGTTAATTTTGATTG1870.2341099440389599No Hit
CTATAAATCAACATAATTTCCACACCCTATTTCTCC1800.2253464701979293No Hit
GTTTGGTTATGTAGATTGTTTGGATAGGTTTTATTG1790.22409454536349635No Hit
ATCTATACAACAATATTTTTCATAAATCCACCCCAT1790.22409454536349635No Hit
ATAACACCAATAAAACCTATCCAAACAATCTACAT1780.22284262052906342No Hit
CAATCAAAATTAACCATCTATACAACAATATTTTTC1780.22284262052906342No Hit
CTATACCTACAACTTTTCATCATACTATTCCAACAT1750.21908684602576461No Hit
TTGGAATAGTATGATGAAAAGTTGTAGGTATAGT1740.2178349211913317No Hit
GATTGTTTGGATAGGTTTTATTGGTGTTATGATGGT1730.21658299635689873No Hit
ATATTTTTCATAAATCCACCCCATAAATCCAATCT1710.21407914668803285No Hit
TAGTAATTATGGTGTTGTGTTGGAGAAATAGGGTGT1710.21407914668803285No Hit
CATACTATTCCAACATATTATTAACAATCCAACAC1690.211575297019167No Hit
CTACCTACTATACCTACAACTTTTCATCATACTATT1680.21032337218473401No Hit
CTATTTCTCCAACACAACACCATAATTACTATAAAC1670.20907144735030106No Hit
CATATTATTAACAATCCAACACATCTACTCACAATC1640.20531567284700225No Hit
CTAAACACACCATTAATAATCTACACTACCTACTAT1630.20406374801256932No Hit
ATTTCTCCAACACAACACCATAATTACTATAAACC1630.20406374801256932No Hit
TGTTGGAATAGTATGATGAAAAGTTGTAGGTATAGT1620.2028118231781364No Hit
TTGTTAATAATATGTTGGAATAGTATGATGAAAAGT1610.20155989834370344No Hit
TAGATGGTTAATTTTGATTGTGAGTAGATGTGTTGG1610.20155989834370344No Hit
GATTGGATTTATGGGGTGGATTTATGAAAAATATTG1600.20030797350927051No Hit
CTATTCCAACATATTATTAACAATCCAACACATCT1600.20030797350927051No Hit
GTAGATGTGTTGGATTGTTAATAATATGTTGGAAT1590.19905604867483756No Hit
ATAACCAAACACCAAAAAACAACACTAACAAACAAC1550.1940483493371058No Hit
ACTCACAATCAAAATTAACCATCTATACAACAATAT1550.1940483493371058No Hit
ACCAATAAAACCTATCCAAACAATCTACATAACCA1530.19154449966823991No Hit
CATCTACTCACAATCAAAATTAACCATCTATACAAC1530.19154449966823991No Hit
AATACCATCCACCTTATAACTACCACTATAAATCA1530.19154449966823991No Hit
TGTGTTGGATTGTTAATAATATGTTGGAATAGTATG1520.190292574833807No Hit
TTATAGTAATTATGGTGTTGTGTTGGAGAAATAGGG1510.18904064999937403No Hit
CCATCATAACACCAATAAAACCTATCCAAACAATCT1490.18653680033050815No Hit
CAATCTACATAACCAAACACCAAAAAACAACACTA1490.18653680033050815No Hit
ATATTATTAACAATCCAACACATCTACTCACAATC1480.18528487549607522No Hit
ATAGATGGTTAATTTTGATTGTGAGTAGATGTGTTG1480.18528487549607522No Hit
TTGGTGTTATGATGGTTTATAGTAATTATGGTGTTG1470.18403295066164227No Hit
GGTTTATAGTAATTATGGTGTTGTGTTGGAGAAAT1460.18278102582720934No Hit
GATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATT1450.18152910099277642No Hit
TTATTAACAATCCAACACATCTACTCACAATCAAA1440.18027717615834343No Hit
GTGTTGTGTTGGAGAAATAGGGTGTGGAAATTATGT1420.17777332648947758No Hit
GGGTGTGGAAATTATGTTGATTTATAGTGGTAGTT1410.17652140165504462No Hit
GATGTGTTGGATTGTTAATAATATGTTGGAATAGT1410.17652140165504462No Hit
GTTGGAATAGTATGATGAAAAGTTGTAGGTATAGT1380.17276562715174582No Hit
GAATAGTATGATGAAAAGTTGTAGGTATAGTAGGT1370.17151370231731286No Hit
ATTATTAACAATCCAACACATCTACTCACAATCAA1370.17151370231731286No Hit
CTACAACTTTTCATCATACTATTCCAACATATTATT1370.17151370231731286No Hit
TTACCATCCACCTTATAACTACCACTATAAATCAAC1360.17026177748287993No Hit
GTGGATTTATGAAAAATATTGTTGTATAGATGGTT1350.16900985264844698No Hit
GTTGTAGGTATAGTAGGTAGTGTAGATTATTAATGG1350.16900985264844698No Hit
TGTATAGATGGTTAATTTTGATTGTGAGTAGATGTG1340.16775792781401405No Hit
ACAATCCAACACATCTACTCACAATCAAAATTAACC1340.16775792781401405No Hit
TGGTGTTGTGTTGGAGAAATAGGGTGTGGAAATTAT1340.16775792781401405No Hit
AATATGTTGGAATAGTATGATGAAAAGTTGTAGGT1340.16775792781401405No Hit
ACAATCAAAATTAACCATCTATACAACAATATTTTT1320.16525407814514817No Hit
TTGTTGTATAGATGGTTAATTTTGATTGTGAGT1320.16525407814514817No Hit
ATAATTTCCACACCCTATTTCTCCAACACAACACC1320.16525407814514817No Hit
GTGTTGGATTGTTAATAATATGTTGGAATAGTATG1320.16525407814514817No Hit
CCATCTATACAACAATATTTTTCATAAATCCACCCC1310.16400215331071522No Hit
GTATAGATGGTTAATTTTGATTGTGAGTAGATGTGT1300.1627502284762823No Hit
TGATGAAAAGTTGTAGGTATAGTAGGTAGTGT1300.1627502284762823No Hit
CACAATCAAAATTAACCATCTATACAACAATATTTT1300.1627502284762823No Hit
ATCTACTCACAATCAAAATTAACCATCTATACAAC1300.1627502284762823No Hit
ATCCAACACATCTACTCACAATCAAAATTAACCATC1300.1627502284762823No Hit
TGTGAGTAGATGTGTTGGATTGTTAATAATATGTTG1280.1602463788074164No Hit
TAATATGTTGGAATAGTATGATGAAAAGTTGTAGGT1270.15899445397298345No Hit
TGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTAT1270.15899445397298345No Hit
CAATCCAACACATCTACTCACAATCAAAATTAACC1260.15774252913855052No Hit
GTAGGTAGTGTAGATTATTAATGGTGTGTTTAGTT1260.15774252913855052No Hit
GGTTTTATTGGTGTTATGATGGTTTATAGTAATTAT1260.15774252913855052No Hit
ATTAACCATCTATACAACAATATTTTTCATAAATCC1250.1564906043041176No Hit
ATAGTAGGTAGTGTAGATTATTAATGGTGTGTTT1250.1564906043041176No Hit
TGGTTAATTTTGATTGTGAGTAGATGTGTTGGATTG1250.1564906043041176No Hit
TATAAACCATCATAACACCAATAAAACCTATCCAA1230.1539867546352517No Hit
ATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATT1220.15273482980081876No Hit
CAAACACCAAAAAACAACACTAACAAACAACTAACC1220.15273482980081876No Hit
ATTAATAATCTACACTACCTACTATACCTACAACTT1220.15273482980081876No Hit
CCTATCCAAACAATCTACATAACCAAACACCAAAA1210.1514829049663858No Hit
GTTTATAGTAATTATGGTGTTGTGTTGGAGAAAT1210.1514829049663858No Hit
AATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGA1180.147727130463087No Hit
CAAAATTAACCATCTATACAACAATATTTTTCATA1160.14522328079422112No Hit
CATCATAACACCAATAAAACCTATCCAAACAATCT1150.14397135595978816No Hit
CCAATAAAACCTATCCAAACAATCTACATAACCAA1150.14397135595978816No Hit
ATACCATCCACCTTATAACTACCACTATAAATCAAC1150.14397135595978816No Hit
TTGGAGAAATAGGGTGTGGAAATTATGTTGATTTAT1140.14271943112535523No Hit
ATACTATTCCAACATATTATTAACAATCCAACACAT1130.1414675062909223No Hit
CAATAAAACCTATCCAAACAATCTACATAACCAAAC1120.14021558145648935No Hit
TTCCAACATATTATTAACAATCCAACACATCTACTC1100.13771173178762347No Hit
TAACAATCCAACACATCTACTCACAATCAAAATTA1090.13645980695319052No Hit
AATAGGGTGTGGAAATTATGTTGATTTATAGTGGT1080.1352078821187576No Hit
TACCATCCACCTTATAACTACCACTATAAATCAAC1080.1352078821187576No Hit
CACCCTATTTCTCCAACACAACACCATAATTACTAT1080.1352078821187576No Hit
ATTTTTCATAAATCCACCCCATAAATCCAATCTAT1070.13395595728432466No Hit
TTTATGAAAAATATTGTTGTATAGATGGTTAATTTT1070.13395595728432466No Hit
TAAACCATCATAACACCAATAAAACCTATCCAAAC1070.13395595728432466No Hit
TATTGTTGTATAGATGGTTAATTTTGATTGTGAGT1070.13395595728432466No Hit
GGAATAGTATGATGAAAAGTTGTAGGTATAGTAGGT1060.1327040324498917No Hit
CTACTCACAATCAAAATTAACCATCTATACAACAAT1060.1327040324498917No Hit
GTGTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTT1050.13145210761545878No Hit
GATGGTTAATTTTGATTGTGAGTAGATGTGTTGGAT1050.13145210761545878No Hit
TGGAATAGTATGATGAAAAGTTGTAGGTATAGTAGG1040.13020018278102582No Hit
CATCATACTATTCCAACATATTATTAACAATCCAAC1030.12894825794659287No Hit
GTTAATAATATGTTGGAATAGTATGATGAAAAGTTG1020.12769633311215994No Hit
TAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGTG1010.12644440827772702No Hit
CCATAATTACTATAAACCATCATAACACCAATAAA1010.12644440827772702No Hit
ATTACTATAAACCATCATAACACCAATAAAACCTAT1010.12644440827772702No Hit
ATTCCAACATATTATTAACAATCCAACACATCTACT1010.12644440827772702No Hit
GTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTTGG1000.12519248344329406No Hit
TATTATTAACAATCCAACACATCTACTCACAATCA1000.12519248344329406No Hit
AAATTAACCATCTATACAACAATATTTTTCATAAAT990.12394055860886113No Hit
TTAACCATCTATACAACAATATTTTTCATAAATCC990.12394055860886113No Hit
ATTAACAATCCAACACATCTACTCACAATCAAAATT970.12143670893999524No Hit
CATAATTACTATAAACCATCATAACACCAATAAAAC970.12143670893999524No Hit
GATTTATGAAAAATATTGTTGTATAGATGGTTAATT950.11893285927112936No Hit
ATAGGGTGTGGAAATTATGTTGATTTATAGTGGT950.11893285927112936No Hit
GGTTAATTTTGATTGTGAGTAGATGTGTTGGATTGT950.11893285927112936No Hit
CCTTATAACTACCACTATAAATCAACATAATTTCC950.11893285927112936No Hit
CACTACCTACTATACCTACAACTTTTCATCATACT940.11768093443669642No Hit
TTTATTGGTGTTATGATGGTTTATAGTAATTATGGT940.11768093443669642No Hit
AATAATATGTTGGAATAGTATGATGAAAAGTTGT930.11642900960226349No Hit
AACACATCTACTCACAATCAAAATTAACCATCTAT920.11517708476783055No Hit
ACATATTATTAACAATCCAACACATCTACTCACAAT900.11267323509896465No Hit
ATTTGATAAATATAGATTGGATTTATGGGGTGGATT900.11267323509896465No Hit
AATCAAAATTAACCATCTATACAACAATATTTTTC890.11142131026453171No Hit
CAACACAACACCATAATTACTATAAACCATCATAAC890.11142131026453171No Hit
CAACATATTATTAACAATCCAACACATCTACTCAC890.11142131026453171No Hit
TACAACAATATTTTTCATAAATCCACCCCATAAATC890.11142131026453171No Hit
ATAATCTACACTACCTACTATACCTACAACTTTTC890.11142131026453171No Hit
TAGGGTGTGGAAATTATGTTGATTTATAGTGGTAGT890.11142131026453171No Hit
GAGAAATAGGGTGTGGAAATTATGTTGATTTATAGT890.11142131026453171No Hit
TGTTAATAATATGTTGGAATAGTATGATGAAAAGTT880.11016938543009877No Hit
TGATGGTTTATAGTAATTATGGTGTTGTGTTGG880.11016938543009877No Hit
ATGAAAAATATTGTTGTATAGATGGTTAATTTTGAT870.10891746059566584No Hit
TATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT870.10891746059566584No Hit
ATTTCCACACCCTATTTCTCCAACACAACACCATA860.1076655357612329No Hit
ACATAACCAAACACCAAAAAACAACACTAACAAAC860.1076655357612329No Hit
ACCATAATTACTATAAACCATCATAACACCAATAA860.1076655357612329No Hit
CATAAATCCACCCCATAAATCCAATCTATATTTATC850.10641361092679996No Hit
TGGAGAAATAGGGTGTGGAAATTATGTTGATTTAT850.10641361092679996No Hit
CAACACCATAATTACTATAAACCATCATAACACCA850.10641361092679996No Hit
TTGGATTGTTAATAATATGTTGGAATAGTATGATG830.10390976125793407No Hit
TAATTTCCACACCCTATTTCTCCAACACAACACCAT830.10390976125793407No Hit
AACATATTATTAACAATCCAACACATCTACTCACA830.10390976125793407No Hit
ACTATAAATCAACATAATTTCCACACCCTATTTCTC830.10390976125793407No Hit
ATTGTTGTATAGATGGTTAATTTTGATTGTGAGT820.10265783642350113No Hit
TGTGTTGGAGAAATAGGGTGTGGAAATTATGTTGAT820.10265783642350113No Hit
ACTTTTCATCATACTATTCCAACATATTATTAACA820.10265783642350113No Hit
CCAACACAACACCATAATTACTATAAACCATCATA820.10265783642350113No Hit
TTAATAATATGTTGGAATAGTATGATGAAAAGTTGT820.10265783642350113No Hit
CACATCTACTCACAATCAAAATTAACCATCTATAC820.10265783642350113No Hit
CACCAATAAAACCTATCCAAACAATCTACATAACC820.10265783642350113No Hit
TATGAAAAATATTGTTGTATAGATGGTTAATTTTG810.1014059115890682No Hit
GGGGTGGATTTATGAAAAATATTGTTGTATAGATGG810.1014059115890682No Hit
AACACAACACCATAATTACTATAAACCATCATAAC800.10015398675463526No Hit
GTTATGTAGATTGTTTGGATAGGTTTTATTGGTGTT800.10015398675463526No Hit
GATTGATAAATATAGATTGGATTTATGGGGTGGATT800.10015398675463526No Hit
ATCATACTATTCCAACATATTATTAACAATCCAAC800.10015398675463526No Hit
ATCTACACTACCTACTATACCTACAACTTTTCATC800.10015398675463526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)