FastQCFastQC Report
Mon 13 Sep 2021
SRR389222_sub2_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389222_sub2_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119828
Sequences flagged as poor quality0
Sequence length20-36
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTAGGTTAGTTGTTTGTTAGTGTTGTTTTTTGG83686.9833427913342465No Hit
GATTTGATAAATATAGATTGGATTTATGGGGTGGAT76866.414193677604567No Hit
AATTACCATCCACCTTATAACTACCACTATAAATC70835.910972393764395No Hit
TTACCAATAACTAACTAAACACACCATTAATAATCT69775.822512267583536No Hit
ATAAAACCTATCCAAACAATCTACATAACCAAACAC9320.7777814867977434No Hit
TACCAATAACTAACTAAACACACCATTAATAATCT7100.5925159395133024No Hit
CTATAAACCATCATAACACCAATAAAACCTATCCA6950.5799979971292186No Hit
GTTGTGTTGGAGAAATAGGGTGTGGAAATTATGTTG6370.5315952865774276No Hit
GTTGGAGAAATAGGGTGTGGAAATTATGTTGATTT5870.4898688119638148No Hit
CTCACAATCAAAATTAACCATCTATACAACAATATT5830.48653069399472576No Hit
GTAATTATGGTGTTGTGTTGGAGAAATAGGGTGTGG5800.48402710551790895No Hit
GTATGATGAAAAGTTGTAGGTATAGTAGGTAGTGT5320.4439696898888406No Hit
GAAATAGGGTGTGGAAATTATGTTGATTTATAGTGG5210.4347898654738458No Hit
GAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTATT5060.422271923089762No Hit
GTTATGATGGTTTATAGTAATTATGGTGTTGTGTTG4940.4122575691824949No Hit
ATGATGAAAAGTTGTAGGTATAGTAGGTAGTGT4900.4089194512134059No Hit
TGGGGTGGATTTATGAAAAATATTGTTGTATAGATG4760.39723603832159426No Hit
ATACAACAATATTTTTCATAAATCCACCCCATAAAT4490.37470374203024337No Hit
ATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGAA4480.3738692125379711No Hit
CATAACCAAACACCAAAAAACAACACTAACAAACA4350.36302032913843174No Hit
ATAATATGTTGGAATAGTATGATGAAAAGTTGTAGG4230.35300597523116467No Hit
ATCCAAACAATCTACATAACCAAACACCAAAAAAC4200.35050238675434786No Hit
CAAACAATCTACATAACCAAACACCAAAAAACAAC4190.34966785726207567No Hit
CTACATAACCAAACACCAAAAAACAACACTAACAA4110.3429916213238976No Hit
CTATCCAAACAATCTACATAACCAAACACCAAAAA4080.3404880328470808No Hit
ATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT3940.3288046199552692No Hit
ATCATAACACCAATAAAACCTATCCAAACAATCTAC3860.32212838401709115No Hit
ATTACCATCCACCTTATAACTACCACTATAAATCA3770.3146176185866409No Hit
GTGGAAATTATGTTGATTTATAGTGGTAGTTATA3660.305437794171646No Hit
AAACAATCTACATAACCAAACACCAAAAAACAACAC3590.29959608772574026No Hit
CATCTATACAACAATATTTTTCATAAATCCACCCC3520.29375438127983444No Hit
CTACACTACCTACTATACCTACAACTTTTCATCAT3490.29125079280301763No Hit
TGAAAAATATTGTTGTATAGATGGTTAATTTTGATT3480.29041626331074544No Hit
TTAACAATCCAACACATCTACTCACAATCAAAATT3430.2862436158493841No Hit
ATCAAAATTAACCATCTATACAACAATATTTTTCAT3380.2820709683880228No Hit
GTTGGATTGTTAATAATATGTTGGAATAGTATGATG3370.28123643889575056No Hit
ATAATTACTATAAACCATCATAACACCAATAAAACC3340.2787328504189338No Hit
TAATAATATGTTGGAATAGTATGATGAAAAGTTGT3310.276229261942117No Hit
AATAAAACCTATCCAAACAATCTACATAACCAAAC3300.27539473244984475No Hit
ATCTACATAACCAAACACCAAAAAACAACACTAAC3220.26871849651166674No Hit
ATTTTGATTGTGAGTAGATGTGTTGGATTGTTAAT3210.2678839670193945No Hit
TAGATTGGATTTATGGGGTGGATTTATGAAAAATAT3150.2628767900657609No Hit
ATAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGT3080.2570350836198551No Hit
GGTATAGTAGGTAGTGTAGATTATTAATGGTGTGTT3070.2562005541275829No Hit
ATAAACCATCATAACACCAATAAAACCTATCCAAAC3050.25453149514303836No Hit
CTATACAACAATATTTTTCATAAATCCACCCCATA3030.25286243615849385No Hit
ATAGATGGTTAATTTTGATTGTGAGTAGATGTGTTG2980.24868978869713254No Hit
GAAAAATATTGTTGTATAGATGGTTAATTTTGATTG2920.243682611743499No Hit
ATATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT2890.24117902326668225No Hit
CAATCAAAATTAACCATCTATACAACAATATTTTTC2800.23366825783623196No Hit
CATAACACCAATAAAACCTATCCAAACAATCTACAT2690.22448843342123712No Hit
CTATACCTACAACTTTTCATCATACTATTCCAACAT2680.22365390392896484No Hit
CTATTCCAACATATTATTAACAATCCAACACATCT2670.2228193744366926No Hit
GTTTGGTTATGTAGATTGTTTGGATAGGTTTTATTG2650.22115031545214808No Hit
ATAACACCAATAAAACCTATCCAAACAATCTACAT2650.22115031545214808No Hit
GATGTGTTGGATTGTTAATAATATGTTGGAATAGT2650.22115031545214808No Hit
TTGTGAGTAGATGTGTTGGATTGTTAATAATATGTT2610.21781219748305908No Hit
CATACTATTCCAACATATTATTAACAATCCAACAC2540.21197049103715326No Hit
TGTTGGAATAGTATGATGAAAAGTTGTAGGTATAGT2540.21197049103715326No Hit
CTACCTACTATACCTACAACTTTTCATCATACTATT2530.211135961544881No Hit
GATTGTTTGGATAGGTTTTATTGGTGTTATGATGGT2510.20946690256033648No Hit
GTAGATGTGTTGGATTGTTAATAATATGTTGGAAT2490.20779784357579195No Hit
TTATAGTAATTATGGTGTTGTGTTGGAGAAATAGGG2480.20696331408351973No Hit
ATCTATACAACAATATTTTTCATAAATCCACCCCAT2450.20445972560670295No Hit
TAGATGGTTAATTTTGATTGTGAGTAGATGTGTTGG2440.20362519611443067No Hit
CAACAATATTTTTCATAAATCCACCCCATAAATCC2420.2019561371298862No Hit
GATTGGATTTATGGGGTGGATTTATGAAAAATATTG2350.19611443068398038No Hit
CTATAAATCAACATAATTTCCACACCCTATTTCTCC2350.19611443068398038No Hit
ATAACCAAACACCAAAAAACAACACTAACAAACAAC2340.1952799011917081No Hit
ATATTATTAACAATCCAACACATCTACTCACAATC2340.1952799011917081No Hit
TGATGAAAAGTTGTAGGTATAGTAGGTAGTGT2330.19444537169943585No Hit
TAGTAATTATGGTGTTGTGTTGGAGAAATAGGGTGT2330.19444537169943585No Hit
CATAATTTCCACACCCTATTTCTCCAACACAACACC2330.19444537169943585No Hit
ATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATT2320.1936108422071636No Hit
TTGGAATAGTATGATGAAAAGTTGTAGGTATAGT2320.1936108422071636No Hit
TTGTTAATAATATGTTGGAATAGTATGATGAAAAGT2290.19110725373034682No Hit
CTATTTCTCCAACACAACACCATAATTACTATAAAC2280.1902727242380746No Hit
ATATTTTTCATAAATCCACCCCATAAATCCAATCT2250.1877691357612578No Hit
ATCTACTCACAATCAAAATTAACCATCTATACAAC2250.1877691357612578No Hit
TGGTGTTGTGTTGGAGAAATAGGGTGTGGAAATTAT2250.1877691357612578No Hit
TTGGTGTTATGATGGTTTATAGTAATTATGGTGTTG2250.1877691357612578No Hit
TTATTAACAATCCAACACATCTACTCACAATCAAA2240.18693460626898556No Hit
TGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTAT2240.18693460626898556No Hit
ACTCACAATCAAAATTAACCATCTATACAACAATAT2220.18526554728444103No Hit
ACCAATAAAACCTATCCAAACAATCTACATAACCA2210.18443101779216878No Hit
GTTGGAATAGTATGATGAAAAGTTGTAGGTATAGT2200.18359648829989653No Hit
CTACAACTTTTCATCATACTATTCCAACATATTATT2170.18109289982307974No Hit
CAATCTACATAACCAAACACCAAAAAACAACACTA2170.18109289982307974No Hit
CCATCATAACACCAATAAAACCTATCCAAACAATCT2150.17942384083853524No Hit
CATATTATTAACAATCCAACACATCTACTCACAATC2120.17692025236171846No Hit
ATTTCTCCAACACAACACCATAATTACTATAAACC2100.17525119337717393No Hit
TTGTTGTATAGATGGTTAATTTTGATTGTGAGT2080.17358213439262943No Hit
ACAATCCAACACATCTACTCACAATCAAAATTAACC2080.17358213439262943No Hit
CATCTACTCACAATCAAAATTAACCATCTATACAAC2080.17358213439262943No Hit
GTGTTGGATTGTTAATAATATGTTGGAATAGTATG2080.17358213439262943No Hit
GGTTTATAGTAATTATGGTGTTGTGTTGGAGAAAT2070.17274760490035718No Hit
GTTGTAGGTATAGTAGGTAGTGTAGATTATTAATGG2060.17191307540808493No Hit
ACAATCAAAATTAACCATCTATACAACAATATTTTT2050.17107854591581267No Hit
CTAAACACACCATTAATAATCTACACTACCTACTAT2030.16940948693126814No Hit
ATAGTAGGTAGTGTAGATTATTAATGGTGTGTTT2030.16940948693126814No Hit
CCATCTATACAACAATATTTTTCATAAATCCACCCC2030.16940948693126814No Hit
ATAATTTCCACACCCTATTTCTCCAACACAACACC2020.1685749574389959No Hit
AATACCATCCACCTTATAACTACCACTATAAATCA1990.1660713689621791No Hit
GGGTGTGGAAATTATGTTGATTTATAGTGGTAGTT1980.1652368394699069No Hit
CCATAATTACTATAAACCATCATAACACCAATAAA1950.16273325099309008No Hit
TACCATCCACCTTATAACTACCACTATAAATCAAC1950.16273325099309008No Hit
TGTGTTGGATTGTTAATAATATGTTGGAATAGTATG1930.16106419200854558No Hit
GGTTTTATTGGTGTTATGATGGTTTATAGTAATTAT1920.16022966251627332No Hit
GTATAGATGGTTAATTTTGATTGTGAGTAGATGTGT1890.15772607403945654No Hit
ATCCAACACATCTACTCACAATCAAAATTAACCATC1890.15772607403945654No Hit
ATACTATTCCAACATATTATTAACAATCCAACACAT1890.15772607403945654No Hit
GAATAGTATGATGAAAAGTTGTAGGTATAGTAGGT1870.15605701505491204No Hit
TAATATGTTGGAATAGTATGATGAAAAGTTGTAGGT1870.15605701505491204No Hit
CACAATCAAAATTAACCATCTATACAACAATATTTT1870.15605701505491204No Hit
CCTATCCAAACAATCTACATAACCAAACACCAAAA1860.1552224855626398No Hit
ATTTTTCATAAATCCACCCCATAAATCCAATCTAT1840.15355342657809526No Hit
ATTAACCATCTATACAACAATATTTTTCATAAATCC1830.152718897085823No Hit
CTACTCACAATCAAAATTAACCATCTATACAACAAT1820.15188436759355076No Hit
GTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTTGG1800.15021530860900623No Hit
GTTAATAATATGTTGGAATAGTATGATGAAAAGTTG1800.15021530860900623No Hit
ATTATTAACAATCCAACACATCTACTCACAATCAA1800.15021530860900623No Hit
CAAACACCAAAAAACAACACTAACAAACAACTAACC1800.15021530860900623No Hit
TGTATAGATGGTTAATTTTGATTGTGAGTAGATGTG1790.14938077911673397No Hit
TTACCATCCACCTTATAACTACCACTATAAATCAAC1780.14854624962446172No Hit
CAATCCAACACATCTACTCACAATCAAAATTAACC1780.14854624962446172No Hit
AATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGA1770.14771172013218947No Hit
GTGGATTTATGAAAAATATTGTTGTATAGATGGTT1770.14771172013218947No Hit
AATATGTTGGAATAGTATGATGAAAAGTTGTAGGT1770.14771172013218947No Hit
GTGTTGTGTTGGAGAAATAGGGTGTGGAAATTATGT1760.14687719063991722No Hit
ATTACTATAAACCATCATAACACCAATAAAACCTAT1720.1435390726708282No Hit
ATACCATCCACCTTATAACTACCACTATAAATCAAC1720.1435390726708282No Hit
CAATAAAACCTATCCAAACAATCTACATAACCAAAC1710.14270454317855594No Hit
ATTAATAATCTACACTACCTACTATACCTACAACTT1700.14187001368628369No Hit
CAAAATTAACCATCTATACAACAATATTTTTCATA1670.1393664252094669No Hit
CATCATAACACCAATAAAACCTATCCAAACAATCT1660.13853189571719465No Hit
TATAAACCATCATAACACCAATAAAACCTATCCAA1660.13853189571719465No Hit
ATAGGGTGTGGAAATTATGTTGATTTATAGTGGT1640.13686283673265012No Hit
TTTATGAAAAATATTGTTGTATAGATGGTTAATTTT1630.13602830724037787No Hit
CCAATAAAACCTATCCAAACAATCTACATAACCAA1630.13602830724037787No Hit
GTTTATAGTAATTATGGTGTTGTGTTGGAGAAAT1630.13602830724037787No Hit
TAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGTG1620.13519377774810562No Hit
TGTGAGTAGATGTGTTGGATTGTTAATAATATGTTG1620.13519377774810562No Hit
TGGAATAGTATGATGAAAAGTTGTAGGTATAGTAGG1620.13519377774810562No Hit
TAACAATCCAACACATCTACTCACAATCAAAATTA1610.13435924825583337No Hit
TTGGAGAAATAGGGTGTGGAAATTATGTTGATTTAT1600.1335247187635611No Hit
GAGAAATAGGGTGTGGAAATTATGTTGATTTATAGT1600.1335247187635611No Hit
GATGGTTAATTTTGATTGTGAGTAGATGTGTTGGAT1590.13269018927128887No Hit
CAACACAACACCATAATTACTATAAACCATCATAAC1580.1318556597790166No Hit
GGAATAGTATGATGAAAAGTTGTAGGTATAGTAGGT1560.13018660079447206No Hit
GATTTATGAAAAATATTGTTGTATAGATGGTTAATT1560.13018660079447206No Hit
TTCCAACATATTATTAACAATCCAACACATCTACTC1550.12935207130219983No Hit
GTAGGTAGTGTAGATTATTAATGGTGTGTTTAGTT1520.12684848282538302No Hit
AATAGGGTGTGGAAATTATGTTGATTTATAGTGGT1520.12684848282538302No Hit
ATAATCTACACTACCTACTATACCTACAACTTTTC1520.12684848282538302No Hit
CATCATACTATTCCAACATATTATTAACAATCCAAC1510.1260139533331108No Hit
ACATAACCAAACACCAAAAAACAACACTAACAAAC1500.12517942384083852No Hit
TAAACCATCATAACACCAATAAAACCTATCCAAAC1490.12434489434856627No Hit
ATTAACAATCCAACACATCTACTCACAATCAAAATT1470.12267583536402177No Hit
GGGGTGGATTTATGAAAAATATTGTTGTATAGATGG1470.12267583536402177No Hit
TAATTTCCACACCCTATTTCTCCAACACAACACCAT1470.12267583536402177No Hit
TGGTTAATTTTGATTGTGAGTAGATGTGTTGGATTG1470.12267583536402177No Hit
TTGGATTGTTAATAATATGTTGGAATAGTATGATG1460.1218413058717495No Hit
AATAATATGTTGGAATAGTATGATGAAAAGTTGT1460.1218413058717495No Hit
GATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATT1460.1218413058717495No Hit
TTAACCATCTATACAACAATATTTTTCATAAATCC1460.1218413058717495No Hit
GGTTAATTTTGATTGTGAGTAGATGTGTTGGATTGT1460.1218413058717495No Hit
GTTGTTTGTTAGTGTTGTTTTTTGGTGTTTGGTTAT1450.12100677637947724No Hit
TCATAACACCAATAAAACCTATCCAAACAATCTAC1440.120172246887205No Hit
TGTAGATTGTTTGGATAGGTTTTATTGGTGTTATG1430.11933771739493274No Hit
TTTATTGGTGTTATGATGGTTTATAGTAATTATGGT1420.11850318790266048No Hit
CACCCTATTTCTCCAACACAACACCATAATTACTAT1410.11766865841038822No Hit
GTGTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTT1400.11683412891811598No Hit
AGGTATAGTAGGTAGTGTAGATTATTAATGGTGTGT1400.11683412891811598No Hit
CAACATATTATTAACAATCCAACACATCTACTCAC1390.11599959942584372No Hit
CTTTTCATCATACTATTCCAACATATTATTAACAAT1370.11433054044129919No Hit
CTACCACTATAAATCAACATAATTTCCACACCCTAT1350.11266148145675468No Hit
TATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT1350.11266148145675468No Hit
ACTATAAATCAACATAATTTCCACACCCTATTTCTC1350.11266148145675468No Hit
TGGAGAAATAGGGTGTGGAAATTATGTTGATTTAT1350.11266148145675468No Hit
TAACACCAATAAAACCTATCCAAACAATCTACATA1330.11099242247221015No Hit
TATTATTAACAATCCAACACATCTACTCACAATCA1330.11099242247221015No Hit
TTGGATTTATGGGGTGGATTTATGAAAAATATTGTT1320.11015789297993792No Hit
CACTACCTACTATACCTACAACTTTTCATCATACT1320.11015789297993792No Hit
CATAAATCCACCCCATAAATCCAATCTATATTTATC1310.10932336348766565No Hit
AACACATCTACTCACAATCAAAATTAACCATCTAT1310.10932336348766565No Hit
AAATTAACCATCTATACAACAATATTTTTCATAAAT1310.10932336348766565No Hit
CCTTATAACTACCACTATAAATCAACATAATTTCC1310.10932336348766565No Hit
TGTGTTGGAGAAATAGGGTGTGGAAATTATGTTGAT1300.10848883399539339No Hit
TAAAACCTATCCAAACAATCTACATAACCAAACACC1290.10765430450312113No Hit
CTACTATACCTACAACTTTTCATCATACTATTCCA1290.10765430450312113No Hit
CATAATTACTATAAACCATCATAACACCAATAAAAC1290.10765430450312113No Hit
TATTGTTGTATAGATGGTTAATTTTGATTGTGAGT1290.10765430450312113No Hit
TGTTAATAATATGTTGGAATAGTATGATGAAAAGTT1280.10681977501084888No Hit
ATCATACTATTCCAACATATTATTAACAATCCAAC1280.10681977501084888No Hit
ATTGTTGTATAGATGGTTAATTTTGATTGTGAGT1270.10598524551857663No Hit
TACAACAATATTTTTCATAAATCCACCCCATAAATC1270.10598524551857663No Hit
CTTATAACTACCACTATAAATCAACATAATTTCCAC1270.10598524551857663No Hit
GAGTAGATGTGTTGGATTGTTAATAATATGTTGGA1260.10515071602630437No Hit
ACACAACACCATAATTACTATAAACCATCATAACAC1260.10515071602630437No Hit
ACCATAATTACTATAAACCATCATAACACCAATAA1260.10515071602630437No Hit
TATGAAAAATATTGTTGTATAGATGGTTAATTTTG1230.1026471275494876No Hit
ATTTGATAAATATAGATTGGATTTATGGGGTGGATT1230.1026471275494876No Hit
GTTTGATAAATATAGATTGGATTTATGGGGTGGATT1220.10181259805721533No Hit
ATGAAAAATATTGTTGTATAGATGGTTAATTTTGAT1220.10181259805721533No Hit
GTGTTGGAGAAATAGGGTGTGGAAATTATGTTGATT1210.1009780685649431No Hit
GGTGTTGTGTTGGAGAAATAGGGTGTGGAAATTATG1210.1009780685649431No Hit
CACAACACCATAATTACTATAAACCATCATAACACC1210.1009780685649431No Hit
TATGTAGATTGTTTGGATAGGTTTTATTGGTGTTAT1200.10014353907267083No Hit
TGGTTTATAGTAATTATGGTGTTGTGTTGGAGAAAT1200.10014353907267083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)