FastQCFastQC Report
Mon 13 Sep 2021
SRR389222_sub1_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389222_sub1_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99829
Sequences flagged as poor quality0
Sequence length20-36
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTAGGTTAGTTGTTTGTTAGTGTTGTTTTTTGG68106.8216650472307645No Hit
GATTTGATAAATATAGATTGGATTTATGGGGTGGAT65576.568231676166245No Hit
AATTACCATCCACCTTATAACTACCACTATAAATC58725.882058319726733No Hit
TTACCAATAACTAACTAAACACACCATTAATAATCT58595.869036051648319No Hit
ATAAAACCTATCCAAACAATCTACATAACCAAACAC7800.7813360847048453No Hit
CTATAAACCATCATAACACCAATAAAACCTATCCA6090.6100431738272446No Hit
TACCAATAACTAACTAAACACACCATTAATAATCT5430.5439301205060654No Hit
GTTGTGTTGGAGAAATAGGGTGTGGAAATTATGTTG5390.5399232687896303No Hit
GTTGGAGAAATAGGGTGTGGAAATTATGTTGATTT4700.47080507668112476No Hit
GTAATTATGGTGTTGTGTTGGAGAAATAGGGTGTGG4680.46880165082290715No Hit
GAAATAGGGTGTGGAAATTATGTTGATTTATAGTGG4570.45778280860271064No Hit
GTATGATGAAAAGTTGTAGGTATAGTAGGTAGTGT4400.4407536888078614No Hit
GTTATGATGGTTTATAGTAATTATGGTGTTGTGTTG4320.43273998537499125No Hit
CTCACAATCAAAATTAACCATCTATACAACAATATT4300.4307365595167737No Hit
ATGATGAAAAGTTGTAGGTATAGTAGGTAGTGT4140.4147091526510333No Hit
ATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGAA3810.3816526259904437No Hit
ATACAACAATATTTTTCATAAATCCACCCCATAAAT3690.3696320708411383No Hit
CATAACCAAACACCAAAAAACAACACTAACAAACA3680.3686303579120296No Hit
GAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTATT3680.3686303579120296No Hit
TGGGGTGGATTTATGAAAAATATTGTTGTATAGATG3670.3676286449829208No Hit
ATAATATGTTGGAATAGTATGATGAAAAGTTGTAGG3590.3596149415500506No Hit
CTATCCAAACAATCTACATAACCAAACACCAAAAA3490.34959781225896286No Hit
ATCCAAACAATCTACATAACCAAACACCAAAAAAC3350.33557383125143997No Hit
CTACATAACCAAACACCAAAAAACAACACTAACAA3320.33256869246411364No Hit
CATCTATACAACAATATTTTTCATAAATCCACCCC3230.32355327610213463No Hit
CAAACAATCTACATAACCAAACACCAAAAAACAAC3230.32355327610213463No Hit
ATTACCATCCACCTTATAACTACCACTATAAATCA3180.31854471145659075No Hit
GTTGGATTGTTAATAATATGTTGGAATAGTATGATG3020.30251730459085036No Hit
AAACAATCTACATAACCAAACACCAAAAAACAACAC3010.3015155916617416No Hit
ATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT2980.29851045287441524No Hit
AATAAAACCTATCCAAACAATCTACATAACCAAAC2950.2955053140870889No Hit
ATAATTACTATAAACCATCATAACACCAATAAAACC2880.28849332358332747No Hit
TAATAATATGTTGGAATAGTATGATGAAAAGTTGT2730.27346762964669585No Hit
ATCAAAATTAACCATCTATACAACAATATTTTTCAT2730.27346762964669585No Hit
GGTATAGTAGGTAGTGTAGATTATTAATGGTGTGTT2710.2714642037884783No Hit
ATCATAACACCAATAAAACCTATCCAAACAATCTAC2690.26946077793026074No Hit
ATAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGT2690.26946077793026074No Hit
ATTTTGATTGTGAGTAGATGTGTTGGATTGTTAAT2680.26845906500115196No Hit
GTGGAAATTATGTTGATTTATAGTGGTAGTTATA2620.26244878742649935No Hit
CTACACTACCTACTATACCTACAACTTTTCATCAT2620.26244878742649935No Hit
CATAACACCAATAAAACCTATCCAAACAATCTACAT2590.25944364863917296No Hit
ATCTACATAACCAAACACCAAAAAACAACACTAAC2530.25343337106452035No Hit
TTAACAATCCAACACATCTACTCACAATCAAAATT2530.25343337106452035No Hit
ATAAACCATCATAACACCAATAAAACCTATCCAAAC2440.24441795470254135No Hit
TAGATTGGATTTATGGGGTGGATTTATGAAAAATAT2320.23239739955323604No Hit
CTATTCCAACATATTATTAACAATCCAACACATCT2320.23239739955323604No Hit
TGAAAAATATTGTTGTATAGATGGTTAATTTTGATT2320.23239739955323604No Hit
CTATACAACAATATTTTTCATAAATCCACCCCATA2300.2303939736950185No Hit
TTGTTAATAATATGTTGGAATAGTATGATGAAAAGT2250.22538540904947457No Hit
ATATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT2220.22238027026214827No Hit
GAAAAATATTGTTGTATAGATGGTTAATTTTGATTG2210.22137855733303952No Hit
CTATACCTACAACTTTTCATCATACTATTCCAACAT2200.2203768444039307No Hit
ATAACCAAACACCAAAAAACAACACTAACAAACAAC2180.21837341854571315No Hit
ATCTATACAACAATATTTTTCATAAATCCACCCCAT2180.21837341854571315No Hit
AATACCATCCACCTTATAACTACCACTATAAATCA2180.21837341854571315No Hit
ATAACACCAATAAAACCTATCCAAACAATCTACAT2150.21536827975838685No Hit
TTATAGTAATTATGGTGTTGTGTTGGAGAAATAGGG2150.21536827975838685No Hit
CTACCTACTATACCTACAACTTTTCATCATACTATT2130.2133648539001693No Hit
CAACAATATTTTTCATAAATCCACCCCATAAATCC2120.21236314097106052No Hit
TTGTGAGTAGATGTGTTGGATTGTTAATAATATGTT2120.21236314097106052No Hit
CTATAAATCAACATAATTTCCACACCCTATTTCTCC2110.21136142804195174No Hit
GATGTGTTGGATTGTTAATAATATGTTGGAATAGT2080.2083562892546254No Hit
TTGGAATAGTATGATGAAAAGTTGTAGGTATAGT2080.2083562892546254No Hit
CATACTATTCCAACATATTATTAACAATCCAACAC2070.20735457632551665No Hit
CATAATTTCCACACCCTATTTCTCCAACACAACACC2060.20635286339640785No Hit
GTAGATGTGTTGGATTGTTAATAATATGTTGGAAT2040.2043494375381903No Hit
CTATTTCTCCAACACAACACCATAATTACTATAAAC2040.2043494375381903No Hit
GTTTGGTTATGTAGATTGTTTGGATAGGTTTTATTG2020.20234601167997274No Hit
TGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTAT2020.20234601167997274No Hit
ATATTTTTCATAAATCCACCCCATAAATCCAATCT2000.20034258582175524No Hit
ATTTCTCCAACACAACACCATAATTACTATAAACC2000.20034258582175524No Hit
TTATTAACAATCCAACACATCTACTCACAATCAAA1970.19733744703442888No Hit
GGTTTATAGTAATTATGGTGTTGTGTTGGAGAAAT1960.1963357341053201No Hit
CTAAACACACCATTAATAATCTACACTACCTACTAT1950.19533402117621132No Hit
CATATTATTAACAATCCAACACATCTACTCACAATC1930.19333059531799376No Hit
ATAGATGGTTAATTTTGATTGTGAGTAGATGTGTTG1930.19333059531799376No Hit
CATCTACTCACAATCAAAATTAACCATCTATACAAC1930.19333059531799376No Hit
TTGTTGTATAGATGGTTAATTTTGATTGTGAGT1910.1913271694597762No Hit
ACAATCAAAATTAACCATCTATACAACAATATTTTT1900.19032545653066743No Hit
ATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATT1890.18932374360155865No Hit
GTTGTAGGTATAGTAGGTAGTGTAGATTATTAATGG1880.1883220306724499No Hit
GATTGGATTTATGGGGTGGATTTATGAAAAATATTG1870.18732031774334112No Hit
TGTTGGAATAGTATGATGAAAAGTTGTAGGTATAGT1870.18732031774334112No Hit
ACCAATAAAACCTATCCAAACAATCTACATAACCA1860.18631860481423235No Hit
TAGTAATTATGGTGTTGTGTTGGAGAAATAGGGTGT1860.18631860481423235No Hit
TTGGTGTTATGATGGTTTATAGTAATTATGGTGTTG1850.18531689188512357No Hit
GATTGTTTGGATAGGTTTTATTGGTGTTATGATGGT1840.1843151789560148No Hit
GAATAGTATGATGAAAAGTTGTAGGTATAGTAGGT1830.183313466026906No Hit
ACAATCCAACACATCTACTCACAATCAAAATTAACC1830.183313466026906No Hit
CAATCAAAATTAACCATCTATACAACAATATTTTTC1820.18231175309779724No Hit
TAGATGGTTAATTTTGATTGTGAGTAGATGTGTTGG1780.17830490138136212No Hit
ACTCACAATCAAAATTAACCATCTATACAACAATAT1770.17730318845225335No Hit
ATATTATTAACAATCCAACACATCTACTCACAATC1750.1752997625940358No Hit
ATACCATCCACCTTATAACTACCACTATAAATCAAC1710.1712929108776007No Hit
ATACTATTCCAACATATTATTAACAATCCAACACAT1710.1712929108776007No Hit
GTTGGAATAGTATGATGAAAAGTTGTAGGTATAGT1700.17029119794849193No Hit
TGATGAAAAGTTGTAGGTATAGTAGGTAGTGT1690.16928948501938315No Hit
CACAATCAAAATTAACCATCTATACAACAATATTTT1690.16928948501938315No Hit
ATCTACTCACAATCAAAATTAACCATCTATACAAC1690.16928948501938315No Hit
TGTGTTGGATTGTTAATAATATGTTGGAATAGTATG1680.16828777209027437No Hit
GTGTTGGATTGTTAATAATATGTTGGAATAGTATG1680.16828777209027437No Hit
CCATCTATACAACAATATTTTTCATAAATCCACCCC1670.1672860591611656No Hit
TGGTGTTGTGTTGGAGAAATAGGGTGTGGAAATTAT1670.1672860591611656No Hit
CAATCTACATAACCAAACACCAAAAAACAACACTA1670.1672860591611656No Hit
AATATGTTGGAATAGTATGATGAAAAGTTGTAGGT1660.16628434623205682No Hit
TTACCATCCACCTTATAACTACCACTATAAATCAAC1650.16528263330294804No Hit
GGGTGTGGAAATTATGTTGATTTATAGTGGTAGTT1630.16327920744473048No Hit
ATAATTTCCACACCCTATTTCTCCAACACAACACC1620.1622774945156217No Hit
AATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGA1600.16027406865740415No Hit
ATAGTAGGTAGTGTAGATTATTAATGGTGTGTTT1590.15927235572829537No Hit
CAATCCAACACATCTACTCACAATCAAAATTAACC1580.1582706427991866No Hit
ATTATTAACAATCCAACACATCTACTCACAATCAA1570.15726892987007782No Hit
CCATCATAACACCAATAAAACCTATCCAAACAATCT1570.15726892987007782No Hit
CCTATCCAAACAATCTACATAACCAAACACCAAAA1570.15726892987007782No Hit
CTACTCACAATCAAAATTAACCATCTATACAACAAT1560.15626721694096907No Hit
TTTATGAAAAATATTGTTGTATAGATGGTTAATTTT1550.1552655040118603No Hit
GTAGGTAGTGTAGATTATTAATGGTGTGTTTAGTT1550.1552655040118603No Hit
CAAACACCAAAAAACAACACTAACAAACAACTAACC1550.1552655040118603No Hit
ATCCAACACATCTACTCACAATCAAAATTAACCATC1550.1552655040118603No Hit
ATTAACCATCTATACAACAATATTTTTCATAAATCC1540.1542637910827515No Hit
TGTATAGATGGTTAATTTTGATTGTGAGTAGATGTG1530.15326207815364273No Hit
ATTAATAATCTACACTACCTACTATACCTACAACTT1520.15226036522453396No Hit
CAATAAAACCTATCCAAACAATCTACATAACCAAAC1510.15125865229542518No Hit
ATAGGGTGTGGAAATTATGTTGATTTATAGTGGT1510.15125865229542518No Hit
GTTAATAATATGTTGGAATAGTATGATGAAAAGTTG1500.1502569393663164No Hit
CAAAATTAACCATCTATACAACAATATTTTTCATA1480.14825351350809887No Hit
CTACAACTTTTCATCATACTATTCCAACATATTATT1480.14825351350809887No Hit
TGTGAGTAGATGTGTTGGATTGTTAATAATATGTTG1470.1472518005789901No Hit
CCAATAAAACCTATCCAAACAATCTACATAACCAA1470.1472518005789901No Hit
TAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGTG1460.1462500876498813No Hit
GGAATAGTATGATGAAAAGTTGTAGGTATAGTAGGT1460.1462500876498813No Hit
GATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATT1460.1462500876498813No Hit
GGTTTTATTGGTGTTATGATGGTTTATAGTAATTAT1460.1462500876498813No Hit
GTATAGATGGTTAATTTTGATTGTGAGTAGATGTGT1450.1452483747207725No Hit
TACCATCCACCTTATAACTACCACTATAAATCAAC1450.1452483747207725No Hit
GTGGATTTATGAAAAATATTGTTGTATAGATGGTT1440.14424666179166373No Hit
TATAAACCATCATAACACCAATAAAACCTATCCAA1440.14424666179166373No Hit
TGGAATAGTATGATGAAAAGTTGTAGGTATAGTAGG1430.14324494886255495No Hit
TTGGAGAAATAGGGTGTGGAAATTATGTTGATTTAT1420.14224323593344618No Hit
ATTACTATAAACCATCATAACACCAATAAAACCTAT1410.1412415230043374No Hit
TTCCAACATATTATTAACAATCCAACACATCTACTC1410.1412415230043374No Hit
CATCATAACACCAATAAAACCTATCCAAACAATCT1400.14023981007522865No Hit
GTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTTGG1380.1382363842170111No Hit
TATTATTAACAATCCAACACATCTACTCACAATCA1380.1382363842170111No Hit
TACAACAATATTTTTCATAAATCCACCCCATAAATC1360.13623295835879354No Hit
CAACACAACACCATAATTACTATAAACCATCATAAC1350.13523124542968476No Hit
TAATATGTTGGAATAGTATGATGAAAAGTTGTAGGT1350.13523124542968476No Hit
CAACATATTATTAACAATCCAACACATCTACTCAC1330.1332278195714672No Hit
CCATAATTACTATAAACCATCATAACACCAATAAA1330.1332278195714672No Hit
ATTTTTCATAAATCCACCCCATAAATCCAATCTAT1300.1302226807841409No Hit
ATGAAAAATATTGTTGTATAGATGGTTAATTTTGAT1280.12821925492592334No Hit
AATAATATGTTGGAATAGTATGATGAAAAGTTGT1280.12821925492592334No Hit
GATTTATGAAAAATATTGTTGTATAGATGGTTAATT1280.12821925492592334No Hit
AATAGGGTGTGGAAATTATGTTGATTTATAGTGGT1270.12721754199681454No Hit
GTGTTGTGTTGGAGAAATAGGGTGTGGAAATTATGT1270.12721754199681454No Hit
CCTTATAACTACCACTATAAATCAACATAATTTCC1270.12721754199681454No Hit
TAACAATCCAACACATCTACTCACAATCAAAATTA1260.12621582906770576No Hit
TGTAGATTGTTTGGATAGGTTTTATTGGTGTTATG1260.12621582906770576No Hit
TGGTTAATTTTGATTGTGAGTAGATGTGTTGGATTG1250.12521411613859698No Hit
CTTTTCATCATACTATTCCAACATATTATTAACAAT1240.12421240320948823No Hit
ATAATCTACACTACCTACTATACCTACAACTTTTC1230.12321069028037945No Hit
CTACTATACCTACAACTTTTCATCATACTATTCCA1220.12220897735127068No Hit
TGTTAATAATATGTTGGAATAGTATGATGAAAAGTT1220.12220897735127068No Hit
CATAATTACTATAAACCATCATAACACCAATAAAAC1220.12220897735127068No Hit
TATTGTTGTATAGATGGTTAATTTTGATTGTGAGT1220.12220897735127068No Hit
GTTTATAGTAATTATGGTGTTGTGTTGGAGAAAT1220.12220897735127068No Hit
GTGTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTT1210.1212072644221619No Hit
GTTATGTAGATTGTTTGGATAGGTTTTATTGGTGTT1200.12020555149305312No Hit
GGTTAATTTTGATTGTGAGTAGATGTGTTGGATTGT1190.11920383856394434No Hit
CATCATACTATTCCAACATATTATTAACAATCCAAC1190.11920383856394434No Hit
ACTATAAATCAACATAATTTCCACACCCTATTTCTC1190.11920383856394434No Hit
ATTAACAATCCAACACATCTACTCACAATCAAAATT1180.11820212563483556No Hit
TGGAGAAATAGGGTGTGGAAATTATGTTGATTTAT1170.11720041270572679No Hit
GTGTTGGAGAAATAGGGTGTGGAAATTATGTTGATT1160.11619869977661802No Hit
ATTGTTAATAATATGTTGGAATAGTATGATGAAA1160.11619869977661802No Hit
CTACCACTATAAATCAACATAATTTCCACACCCTAT1150.11519698684750924No Hit
CATAAATCCACCCCATAAATCCAATCTATATTTATC1140.11419527391840047No Hit
TTGGATTGTTAATAATATGTTGGAATAGTATGATG1140.11419527391840047No Hit
ATAAATCAACATAATTTCCACACCCTATTTCTCCA1140.11419527391840047No Hit
TATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT1130.11319356098929169No Hit
GAGAAATAGGGTGTGGAAATTATGTTGATTTATAGT1130.11319356098929169No Hit
CAACACCATAATTACTATAAACCATCATAACACCA1130.11319356098929169No Hit
TTAACCATCTATACAACAATATTTTTCATAAATCC1120.11219184806018291No Hit
ACACAACACCATAATTACTATAAACCATCATAACAC1120.11219184806018291No Hit
CACCCTATTTCTCCAACACAACACCATAATTACTAT1120.11219184806018291No Hit
ACATAACCAAACACCAAAAAACAACACTAACAAAC1110.11119013513107413No Hit
TTGGATTTATGGGGTGGATTTATGAAAAATATTGTT1100.11018842220196536No Hit
TAGGGTGTGGAAATTATGTTGATTTATAGTGGTAGT1100.11018842220196536No Hit
AATCTACATAACCAAACACCAAAAAACAACACTAAC1080.10818499634374781No Hit
TGTGTTGGAGAAATAGGGTGTGGAAATTATGTTGAT1060.10618157048553026No Hit
AAATTAACCATCTATACAACAATATTTTTCATAAAT1060.10618157048553026No Hit
GGGGTGGATTTATGAAAAATATTGTTGTATAGATGG1050.10517985755642148No Hit
TAATTTCCACACCCTATTTCTCCAACACAACACCAT1050.10517985755642148No Hit
TAAACCATCATAACACCAATAAAACCTATCCAAAC1050.10517985755642148No Hit
GATGGTTAATTTTGATTGTGAGTAGATGTGTTGGAT1050.10517985755642148No Hit
ACATATTATTAACAATCCAACACATCTACTCACAAT1030.10317643169820392No Hit
GTTGTTTGTTAGTGTTGTTTTTTGGTGTTTGGTTAT1020.10217471876909515No Hit
ATTCCAACATATTATTAACAATCCAACACATCTACT1020.10217471876909515No Hit
TTTATTGGTGTTATGATGGTTTATAGTAATTATGGT1010.10117300583998637No Hit
ACCATAATTACTATAAACCATCATAACACCAATAA1010.10117300583998637No Hit
AATCAAAATTAACCATCTATACAACAATATTTTTC1000.10017129291087762No Hit
GATTGATAAATATAGATTGGATTTATGGGGTGGATT1000.10017129291087762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)