FastQCFastQC Report
Mon 13 Sep 2021
SRR389222_sub3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389222_sub3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80000
Sequences flagged as poor quality0
Sequence length36
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTAGGTTAGTTGTTTGTTAGTGTTGTTTTTTGG56737.0912500000000005No Hit
GATTTGATAAATATAGATTGGATTTATGGGGTGGAT52516.56375No Hit
AATTACCATCCACCTTATAACTACCACTATAAATCA47615.95125No Hit
TTACCAATAACTAACTAAACACACCATTAATAATCT46805.8500000000000005No Hit
ATAAAACCTATCCAAACAATCTACATAACCAAACAC6020.7525No Hit
TACCAATAACTAACTAAACACACCATTAATAATCTA5010.62625No Hit
CTATAAACCATCATAACACCAATAAAACCTATCCAA4920.615No Hit
GTTGTGTTGGAGAAATAGGGTGTGGAAATTATGTTG4250.53125No Hit
GAAATAGGGTGTGGAAATTATGTTGATTTATAGTGG3750.46875No Hit
GTTGGAGAAATAGGGTGTGGAAATTATGTTGATTTA3560.445No Hit
GTAATTATGGTGTTGTGTTGGAGAAATAGGGTGTGG3490.43625No Hit
ATGATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGA3460.4325No Hit
GTTATGATGGTTTATAGTAATTATGGTGTTGTGTTG3390.42375No Hit
CTCACAATCAAAATTAACCATCTATACAACAATATT3360.42No Hit
GTATGATGAAAAGTTGTAGGTATAGTAGGTAGTGTA3350.41875No Hit
ATACAACAATATTTTTCATAAATCCACCCCATAAAT3300.41250000000000003No Hit
GAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTATT3280.41000000000000003No Hit
ATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGAAA3180.3975No Hit
CATAACCAAACACCAAAAAACAACACTAACAAACAA3120.38999999999999996No Hit
TGGGGTGGATTTATGAAAAATATTGTTGTATAGATG3030.37875000000000003No Hit
CAAACAATCTACATAACCAAACACCAAAAAACAACA3020.3775No Hit
ATCCAAACAATCTACATAACCAAACACCAAAAAACA2910.36375No Hit
CTACATAACCAAACACCAAAAAACAACACTAACAAA2830.35375No Hit
ATAATATGTTGGAATAGTATGATGAAAAGTTGTAGG2800.35000000000000003No Hit
ATTACCATCCACCTTATAACTACCACTATAAATCAA2780.3475No Hit
CTATCCAAACAATCTACATAACCAAACACCAAAAAA2660.33249999999999996No Hit
AAACAATCTACATAACCAAACACCAAAAAACAACAC2610.32625000000000004No Hit
CATCTATACAACAATATTTTTCATAAATCCACCCCA2580.3225No Hit
ATCATAACACCAATAAAACCTATCCAAACAATCTAC2550.31875No Hit
GGTATAGTAGGTAGTGTAGATTATTAATGGTGTGTT2480.31No Hit
GTTGGATTGTTAATAATATGTTGGAATAGTATGATG2460.3075No Hit
ATCAAAATTAACCATCTATACAACAATATTTTTCAT2390.29875No Hit
ATGTTGGAATAGTATGATGAAAAGTTGTAGGTATAG2350.29375No Hit
ATAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGT2330.29125No Hit
TAGATTGGATTTATGGGGTGGATTTATGAAAAATAT2290.28625No Hit
GTGGAAATTATGTTGATTTATAGTGGTAGTTATAAG2290.28625No Hit
ATTTTGATTGTGAGTAGATGTGTTGGATTGTTAATA2270.28375No Hit
TGAAAAATATTGTTGTATAGATGGTTAATTTTGATT2240.27999999999999997No Hit
ATAAACCATCATAACACCAATAAAACCTATCCAAAC2240.27999999999999997No Hit
TAATAATATGTTGGAATAGTATGATGAAAAGTTGTA2230.27875No Hit
TTAACAATCCAACACATCTACTCACAATCAAAATTA2220.2775No Hit
AATAAAACCTATCCAAACAATCTACATAACCAAACA2120.265No Hit
CTACACTACCTACTATACCTACAACTTTTCATCATA2080.26No Hit
CAACAATATTTTTCATAAATCCACCCCATAAATCCA2060.2575No Hit
CTATACAACAATATTTTTCATAAATCCACCCCATAA2040.255No Hit
ATATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT2020.2525No Hit
ATCTACATAACCAAACACCAAAAAACAACACTAACA1990.24875000000000003No Hit
CATAACACCAATAAAACCTATCCAAACAATCTACAT1950.24375No Hit
CATAATTTCCACACCCTATTTCTCCAACACAACACC1940.2425No Hit
GAAAAATATTGTTGTATAGATGGTTAATTTTGATTG1900.2375No Hit
TTGTGAGTAGATGTGTTGGATTGTTAATAATATGTT1880.23500000000000001No Hit
ATAATTACTATAAACCATCATAACACCAATAAAACC1880.23500000000000001No Hit
GTTTGGTTATGTAGATTGTTTGGATAGGTTTTATTG1830.22875No Hit
CTATAAATCAACATAATTTCCACACCCTATTTCTCC1810.22625No Hit
TTGGAATAGTATGATGAAAAGTTGTAGGTATAGTAG1800.22499999999999998No Hit
ATCTATACAACAATATTTTTCATAAATCCACCCCAT1800.22499999999999998No Hit
CAATCAAAATTAACCATCTATACAACAATATTTTTC1780.2225No Hit
ATAACACCAATAAAACCTATCCAAACAATCTACATA1780.2225No Hit
CTATACCTACAACTTTTCATCATACTATTCCAACAT1770.22125No Hit
TAGTAATTATGGTGTTGTGTTGGAGAAATAGGGTGT1760.22No Hit
CATACTATTCCAACATATTATTAACAATCCAACACA1730.21625No Hit
ATATTTTTCATAAATCCACCCCATAAATCCAATCTA1730.21625No Hit
GATTGTTTGGATAGGTTTTATTGGTGTTATGATGGT1730.21625No Hit
CTATTCCAACATATTATTAACAATCCAACACATCTA1710.21375No Hit
TGTTGGAATAGTATGATGAAAAGTTGTAGGTATAGT1700.21250000000000002No Hit
CTATTTCTCCAACACAACACCATAATTACTATAAAC1700.21250000000000002No Hit
CTACCTACTATACCTACAACTTTTCATCATACTATT1680.21No Hit
TTGTTAATAATATGTTGGAATAGTATGATGAAAAGT1660.2075No Hit
CATATTATTAACAATCCAACACATCTACTCACAATC1660.2075No Hit
CTAAACACACCATTAATAATCTACACTACCTACTAT1650.20625000000000002No Hit
ATTTCTCCAACACAACACCATAATTACTATAAACCA1650.20625000000000002No Hit
ACTCACAATCAAAATTAACCATCTATACAACAATAT1620.20249999999999999No Hit
GTAGATGTGTTGGATTGTTAATAATATGTTGGAATA1620.20249999999999999No Hit
GATTGGATTTATGGGGTGGATTTATGAAAAATATTG1610.20124999999999998No Hit
ACCAATAAAACCTATCCAAACAATCTACATAACCAA1610.20124999999999998No Hit
TAGATGGTTAATTTTGATTGTGAGTAGATGTGTTGG1610.20124999999999998No Hit
ATAACCAAACACCAAAAAACAACACTAACAAACAAC1590.19875No Hit
AATACCATCCACCTTATAACTACCACTATAAATCAA1590.19875No Hit
TGTGTTGGATTGTTAATAATATGTTGGAATAGTATG1570.19624999999999998No Hit
TTATTAACAATCCAACACATCTACTCACAATCAAAA1560.19499999999999998No Hit
CATCTACTCACAATCAAAATTAACCATCTATACAAC1560.19499999999999998No Hit
TTATAGTAATTATGGTGTTGTGTTGGAGAAATAGGG1550.19375No Hit
CAATCTACATAACCAAACACCAAAAAACAACACTAA1540.1925No Hit
GGTTTATAGTAATTATGGTGTTGTGTTGGAGAAATA1520.19No Hit
GATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATT1510.18875No Hit
CCATCATAACACCAATAAAACCTATCCAAACAATCT1500.1875No Hit
ATATTATTAACAATCCAACACATCTACTCACAATCA1490.18625No Hit
GAATAGTATGATGAAAAGTTGTAGGTATAGTAGGTA1480.185No Hit
ATAGATGGTTAATTTTGATTGTGAGTAGATGTGTTG1480.185No Hit
TTGGTGTTATGATGGTTTATAGTAATTATGGTGTTG1480.185No Hit
GTTGGAATAGTATGATGAAAAGTTGTAGGTATAGTA1460.1825No Hit
GATGTGTTGGATTGTTAATAATATGTTGGAATAGTA1430.17875No Hit
GGGTGTGGAAATTATGTTGATTTATAGTGGTAGTTA1430.17875No Hit
GTGTTGTGTTGGAGAAATAGGGTGTGGAAATTATGT1430.17875No Hit
CTACAACTTTTCATCATACTATTCCAACATATTATT1420.17750000000000002No Hit
TGGTGTTGTGTTGGAGAAATAGGGTGTGGAAATTAT1420.17750000000000002No Hit
ATTATTAACAATCCAACACATCTACTCACAATCAAA1420.17750000000000002No Hit
TTGTTGTATAGATGGTTAATTTTGATTGTGAGTAGA1410.17625No Hit
TGATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGAT1400.17500000000000002No Hit
TGTATAGATGGTTAATTTTGATTGTGAGTAGATGTG1380.1725No Hit
TTACCATCCACCTTATAACTACCACTATAAATCAAC1380.1725No Hit
GTGGATTTATGAAAAATATTGTTGTATAGATGGTTA1380.1725No Hit
AATATGTTGGAATAGTATGATGAAAAGTTGTAGGTA1380.1725No Hit
GTTGTAGGTATAGTAGGTAGTGTAGATTATTAATGG1370.17125No Hit
GTGTTGGATTGTTAATAATATGTTGGAATAGTATGA1350.16875No Hit
TAATATGTTGGAATAGTATGATGAAAAGTTGTAGGT1350.16875No Hit
ACAATCCAACACATCTACTCACAATCAAAATTAACC1340.1675No Hit
ATAATTTCCACACCCTATTTCTCCAACACAACACCA1340.1675No Hit
ATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTA1330.16624999999999998No Hit
TGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTAT1330.16624999999999998No Hit
ACAATCAAAATTAACCATCTATACAACAATATTTTT1320.165No Hit
TATAAACCATCATAACACCAATAAAACCTATCCAAA1320.165No Hit
GTATAGATGGTTAATTTTGATTGTGAGTAGATGTGT1310.16375No Hit
CCTATCCAAACAATCTACATAACCAAACACCAAAAA1310.16375No Hit
CACAATCAAAATTAACCATCTATACAACAATATTTT1310.16375No Hit
CCATCTATACAACAATATTTTTCATAAATCCACCCC1310.16375No Hit
ATCTACTCACAATCAAAATTAACCATCTATACAACA1300.1625No Hit
ATCCAACACATCTACTCACAATCAAAATTAACCATC1300.1625No Hit
TGGTTAATTTTGATTGTGAGTAGATGTGTTGGATTG1290.16125No Hit
GGTTTTATTGGTGTTATGATGGTTTATAGTAATTAT1290.16125No Hit
TGTGAGTAGATGTGTTGGATTGTTAATAATATGTTG1280.16No Hit
GTTTATAGTAATTATGGTGTTGTGTTGGAGAAATAG1270.15875No Hit
ATAGTAGGTAGTGTAGATTATTAATGGTGTGTTTAG1270.15875No Hit
GTAGGTAGTGTAGATTATTAATGGTGTGTTTAGTTA1270.15875No Hit
ATTAACCATCTATACAACAATATTTTTCATAAATCC1250.15625No Hit
CAATCCAACACATCTACTCACAATCAAAATTAACCA1250.15625No Hit
AATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGAA1240.155No Hit
CAAACACCAAAAAACAACACTAACAAACAACTAACC1220.1525No Hit
ATTAATAATCTACACTACCTACTATACCTACAACTT1220.1525No Hit
TTGGAGAAATAGGGTGTGGAAATTATGTTGATTTAT1180.1475No Hit
ATACCATCCACCTTATAACTACCACTATAAATCAAC1180.1475No Hit
CAAAATTAACCATCTATACAACAATATTTTTCATAA1180.1475No Hit
CAATAAAACCTATCCAAACAATCTACATAACCAAAC1170.14625No Hit
TTCCAACATATTATTAACAATCCAACACATCTACTC1170.14625No Hit
CATCATAACACCAATAAAACCTATCCAAACAATCTA1160.145No Hit
CCAATAAAACCTATCCAAACAATCTACATAACCAAA1160.145No Hit
ATACTATTCCAACATATTATTAACAATCCAACACAT1160.145No Hit
AATAGGGTGTGGAAATTATGTTGATTTATAGTGGTA1150.14375No Hit
GGAATAGTATGATGAAAAGTTGTAGGTATAGTAGGT1140.14250000000000002No Hit
TATTGTTGTATAGATGGTTAATTTTGATTGTGAGTA1130.14125No Hit
TGGAATAGTATGATGAAAAGTTGTAGGTATAGTAGG1120.13999999999999999No Hit
TACCATCCACCTTATAACTACCACTATAAATCAACA1120.13999999999999999No Hit
TAACAATCCAACACATCTACTCACAATCAAAATTAA1110.13875No Hit
TAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGTG1100.13749999999999998No Hit
TAAACCATCATAACACCAATAAAACCTATCCAAACA1090.13625No Hit
CCATAATTACTATAAACCATCATAACACCAATAAAA1090.13625No Hit
CTACTCACAATCAAAATTAACCATCTATACAACAAT1090.13625No Hit
ATTTTTCATAAATCCACCCCATAAATCCAATCTATA1080.135No Hit
CACCCTATTTCTCCAACACAACACCATAATTACTAT1080.135No Hit
TTTATGAAAAATATTGTTGTATAGATGGTTAATTTT1070.13374999999999998No Hit
GTGTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTT1050.13125No Hit
ATTACTATAAACCATCATAACACCAATAAAACCTAT1050.13125No Hit
GATGGTTAATTTTGATTGTGAGTAGATGTGTTGGAT1050.13125No Hit
CATCATACTATTCCAACATATTATTAACAATCCAAC1040.13No Hit
GTTAATAATATGTTGGAATAGTATGATGAAAAGTTG1030.12875No Hit
ATTCCAACATATTATTAACAATCCAACACATCTACT1030.12875No Hit
TATTATTAACAATCCAACACATCTACTCACAATCAA1010.12625No Hit
GTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTTGG1000.125No Hit
ATTAACAATCCAACACATCTACTCACAATCAAAATT1000.125No Hit
TTAACCATCTATACAACAATATTTTTCATAAATCCA1000.125No Hit
AAATTAACCATCTATACAACAATATTTTTCATAAAT990.12375000000000001No Hit
CATAATTACTATAAACCATCATAACACCAATAAAAC980.1225No Hit
TTTATTGGTGTTATGATGGTTTATAGTAATTATGGT980.1225No Hit
GAGAAATAGGGTGTGGAAATTATGTTGATTTATAGT980.1225No Hit
CCTTATAACTACCACTATAAATCAACATAATTTCCA960.12No Hit
GATTTATGAAAAATATTGTTGTATAGATGGTTAATT960.12No Hit
GGTTAATTTTGATTGTGAGTAGATGTGTTGGATTGT950.11875No Hit
ATAGGGTGTGGAAATTATGTTGATTTATAGTGGTAG950.11875No Hit
AACACATCTACTCACAATCAAAATTAACCATCTATA940.11750000000000001No Hit
CACTACCTACTATACCTACAACTTTTCATCATACTA940.11750000000000001No Hit
AATAATATGTTGGAATAGTATGATGAAAAGTTGTAG930.11624999999999999No Hit
CAACACAACACCATAATTACTATAAACCATCATAAC920.11499999999999999No Hit
ACATATTATTAACAATCCAACACATCTACTCACAAT920.11499999999999999No Hit
TGTTAATAATATGTTGGAATAGTATGATGAAAAGTT920.11499999999999999No Hit
TAGGGTGTGGAAATTATGTTGATTTATAGTGGTAGT920.11499999999999999No Hit
CAACATATTATTAACAATCCAACACATCTACTCACA910.11375No Hit
TATGTTGGAATAGTATGATGAAAAGTTGTAGGTATA910.11375No Hit
ATTTGATAAATATAGATTGGATTTATGGGGTGGATT910.11375No Hit
CAACACCATAATTACTATAAACCATCATAACACCAA900.11249999999999999No Hit
AATCAAAATTAACCATCTATACAACAATATTTTTCA900.11249999999999999No Hit
ATAATCTACACTACCTACTATACCTACAACTTTTCA900.11249999999999999No Hit
ATTTCCACACCCTATTTCTCCAACACAACACCATAA900.11249999999999999No Hit
TACAACAATATTTTTCATAAATCCACCCCATAAATC890.11125No Hit
ATGAAAAATATTGTTGTATAGATGGTTAATTTTGAT880.11No Hit
TTGGATTGTTAATAATATGTTGGAATAGTATGATGA880.11No Hit
TGATGGTTTATAGTAATTATGGTGTTGTGTTGGAGA880.11No Hit
ACATAACCAAACACCAAAAAACAACACTAACAAACA880.11No Hit
ACCATAATTACTATAAACCATCATAACACCAATAAA870.10874999999999999No Hit
CATAAATCCACCCCATAAATCCAATCTATATTTATC860.1075No Hit
AACATATTATTAACAATCCAACACATCTACTCACAA860.1075No Hit
TGGAGAAATAGGGTGTGGAAATTATGTTGATTTATA860.1075No Hit
TGGTTTATAGTAATTATGGTGTTGTGTTGGAGAAAT850.10625000000000001No Hit
CCAACACAACACCATAATTACTATAAACCATCATAA850.10625000000000001No Hit
ACTTTTCATCATACTATTCCAACATATTATTAACAA840.105No Hit
TTAATAATATGTTGGAATAGTATGATGAAAAGTTGT840.105No Hit
TAATTTCCACACCCTATTTCTCCAACACAACACCAT840.105No Hit
ATTGTTGTATAGATGGTTAATTTTGATTGTGAGTAG830.10375No Hit
CACATCTACTCACAATCAAAATTAACCATCTATACA830.10375No Hit
ACTATAAATCAACATAATTTCCACACCCTATTTCTC830.10375No Hit
TGTGTTGGAGAAATAGGGTGTGGAAATTATGTTGAT820.10250000000000001No Hit
GATTGTTAATAATATGTTGGAATAGTATGATGAAAA820.10250000000000001No Hit
CTACTATACCTACAACTTTTCATCATACTATTCCAA820.10250000000000001No Hit
TATGAAAAATATTGTTGTATAGATGGTTAATTTTGA820.10250000000000001No Hit
GGGGTGGATTTATGAAAAATATTGTTGTATAGATGG810.10124999999999999No Hit
TGTAGATTGTTTGGATAGGTTTTATTGGTGTTATGA810.10124999999999999No Hit
CACCAATAAAACCTATCCAAACAATCTACATAACCA810.10124999999999999No Hit
ATCATACTATTCCAACATATTATTAACAATCCAACA810.10124999999999999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)