FastQCFastQC Report
Mon 13 Sep 2021
SRR389222_sub1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR389222_sub1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100000
Sequences flagged as poor quality0
Sequence length36
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTAGGTTAGTTGTTTGTTAGTGTTGTTTTTTGG68506.8500000000000005No Hit
GATTTGATAAATATAGATTGGATTTATGGGGTGGAT67336.7330000000000005No Hit
AATTACCATCCACCTTATAACTACCACTATAAATCA59565.956No Hit
TTACCAATAACTAACTAAACACACCATTAATAATCT59335.933No Hit
ATAAAACCTATCCAAACAATCTACATAACCAAACAC7860.786No Hit
CTATAAACCATCATAACACCAATAAAACCTATCCAA6380.638No Hit
GTTGTGTTGGAGAAATAGGGTGTGGAAATTATGTTG5530.553No Hit
TACCAATAACTAACTAAACACACCATTAATAATCTA5510.551No Hit
GTATGATGAAAAGTTGTAGGTATAGTAGGTAGTGTA4810.481No Hit
GTAATTATGGTGTTGTGTTGGAGAAATAGGGTGTGG4770.477No Hit
GTTGGAGAAATAGGGTGTGGAAATTATGTTGATTTA4700.47000000000000003No Hit
GAAATAGGGTGTGGAAATTATGTTGATTTATAGTGG4630.46299999999999997No Hit
CTCACAATCAAAATTAACCATCTATACAACAATATT4420.44200000000000006No Hit
GTTATGATGGTTTATAGTAATTATGGTGTTGTGTTG4320.432No Hit
ATGATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGA4300.43No Hit
ATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGAAA4170.41700000000000004No Hit
CATAACCAAACACCAAAAAACAACACTAACAAACAA3930.393No Hit
TGGGGTGGATTTATGAAAAATATTGTTGTATAGATG3770.377No Hit
ATACAACAATATTTTTCATAAATCCACCCCATAAAT3770.377No Hit
GAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTATT3730.373No Hit
CTACATAACCAAACACCAAAAAACAACACTAACAAA3660.366No Hit
ATAATATGTTGGAATAGTATGATGAAAAGTTGTAGG3630.363No Hit
CTATCCAAACAATCTACATAACCAAACACCAAAAAA3600.36No Hit
ATCCAAACAATCTACATAACCAAACACCAAAAAACA3400.33999999999999997No Hit
CAAACAATCTACATAACCAAACACCAAAAAACAACA3350.335No Hit
ATTACCATCCACCTTATAACTACCACTATAAATCAA3300.33No Hit
CATCTATACAACAATATTTTTCATAAATCCACCCCA3290.329No Hit
GTTGGATTGTTAATAATATGTTGGAATAGTATGATG3100.31No Hit
AAACAATCTACATAACCAAACACCAAAAAACAACAC3030.303No Hit
ATGTTGGAATAGTATGATGAAAAGTTGTAGGTATAG3020.302No Hit
AATAAAACCTATCCAAACAATCTACATAACCAAACA2970.297No Hit
ATAATTACTATAAACCATCATAACACCAATAAAACC2940.294No Hit
TAATAATATGTTGGAATAGTATGATGAAAAGTTGTA2840.28400000000000003No Hit
ATAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGT2840.28400000000000003No Hit
ATCAAAATTAACCATCTATACAACAATATTTTTCAT2790.27899999999999997No Hit
GGTATAGTAGGTAGTGTAGATTATTAATGGTGTGTT2740.27399999999999997No Hit
ATCATAACACCAATAAAACCTATCCAAACAATCTAC2710.271No Hit
ATTTTGATTGTGAGTAGATGTGTTGGATTGTTAATA2710.271No Hit
GTGGAAATTATGTTGATTTATAGTGGTAGTTATAAG2700.27No Hit
CTACACTACCTACTATACCTACAACTTTTCATCATA2640.264No Hit
CATAACACCAATAAAACCTATCCAAACAATCTACAT2610.261No Hit
ATCTACATAACCAAACACCAAAAAACAACACTAACA2580.258No Hit
TAGATTGGATTTATGGGGTGGATTTATGAAAAATAT2550.255No Hit
TTAACAATCCAACACATCTACTCACAATCAAAATTA2540.254No Hit
ATAAACCATCATAACACCAATAAAACCTATCCAAAC2490.249No Hit
CTATTCCAACATATTATTAACAATCCAACACATCTA2420.242No Hit
CTATACAACAATATTTTTCATAAATCCACCCCATAA2420.242No Hit
TGAAAAATATTGTTGTATAGATGGTTAATTTTGATT2320.232No Hit
TTGTTAATAATATGTTGGAATAGTATGATGAAAAGT2310.231No Hit
AATACCATCCACCTTATAACTACCACTATAAATCAA2260.22599999999999998No Hit
GAAAAATATTGTTGTATAGATGGTTAATTTTGATTG2250.22499999999999998No Hit
ATAACCAAACACCAAAAAACAACACTAACAAACAAC2240.22399999999999998No Hit
ATATGTTGGAATAGTATGATGAAAAGTTGTAGGTAT2240.22399999999999998No Hit
CTATACCTACAACTTTTCATCATACTATTCCAACAT2230.22300000000000003No Hit
ATCTATACAACAATATTTTTCATAAATCCACCCCAT2210.22100000000000003No Hit
ATAACACCAATAAAACCTATCCAAACAATCTACATA2190.219No Hit
TTGGAATAGTATGATGAAAAGTTGTAGGTATAGTAG2180.218No Hit
TTATAGTAATTATGGTGTTGTGTTGGAGAAATAGGG2180.218No Hit
CTACCTACTATACCTACAACTTTTCATCATACTATT2150.215No Hit
CAACAATATTTTTCATAAATCCACCCCATAAATCCA2130.213No Hit
TTGTGAGTAGATGTGTTGGATTGTTAATAATATGTT2130.213No Hit
GATGTGTTGGATTGTTAATAATATGTTGGAATAGTA2120.212No Hit
CTATAAATCAACATAATTTCCACACCCTATTTCTCC2110.211No Hit
CATACTATTCCAACATATTATTAACAATCCAACACA2100.21No Hit
TGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTAT2100.21No Hit
GTTTGGTTATGTAGATTGTTTGGATAGGTTTTATTG2080.208No Hit
GTAGATGTGTTGGATTGTTAATAATATGTTGGAATA2080.208No Hit
TTATTAACAATCCAACACATCTACTCACAATCAAAA2080.208No Hit
CATAATTTCCACACCCTATTTCTCCAACACAACACC2070.207No Hit
ATTTCTCCAACACAACACCATAATTACTATAAACCA2050.20500000000000002No Hit
CTATTTCTCCAACACAACACCATAATTACTATAAAC2050.20500000000000002No Hit
GGTTTATAGTAATTATGGTGTTGTGTTGGAGAAATA2040.20400000000000001No Hit
ATATTTTTCATAAATCCACCCCATAAATCCAATCTA2030.203No Hit
TGTTGGAATAGTATGATGAAAAGTTGTAGGTATAGT2020.202No Hit
TTGTTGTATAGATGGTTAATTTTGATTGTGAGTAGA2000.2No Hit
CTAAACACACCATTAATAATCTACACTACCTACTAT1990.199No Hit
GAATAGTATGATGAAAAGTTGTAGGTATAGTAGGTA1960.196No Hit
CATATTATTAACAATCCAACACATCTACTCACAATC1960.196No Hit
CATCTACTCACAATCAAAATTAACCATCTATACAAC1960.196No Hit
ATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATTA1950.19499999999999998No Hit
ATAGATGGTTAATTTTGATTGTGAGTAGATGTGTTG1950.19499999999999998No Hit
GATTGGATTTATGGGGTGGATTTATGAAAAATATTG1940.194No Hit
TAGTAATTATGGTGTTGTGTTGGAGAAATAGGGTGT1940.194No Hit
ACAATCAAAATTAACCATCTATACAACAATATTTTT1930.193No Hit
GTTGTAGGTATAGTAGGTAGTGTAGATTATTAATGG1920.192No Hit
ACCAATAAAACCTATCCAAACAATCTACATAACCAA1920.192No Hit
TTGGTGTTATGATGGTTTATAGTAATTATGGTGTTG1880.188No Hit
ACTCACAATCAAAATTAACCATCTATACAACAATAT1860.186No Hit
GATTGTTTGGATAGGTTTTATTGGTGTTATGATGGT1840.184No Hit
ACAATCCAACACATCTACTCACAATCAAAATTAACC1830.183No Hit
CAATCAAAATTAACCATCTATACAACAATATTTTTC1820.182No Hit
TAGATGGTTAATTTTGATTGTGAGTAGATGTGTTGG1790.179No Hit
GTTGGAATAGTATGATGAAAAGTTGTAGGTATAGTA1770.17700000000000002No Hit
ATATTATTAACAATCCAACACATCTACTCACAATCA1770.17700000000000002No Hit
TGATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGAT1760.17600000000000002No Hit
ATACTATTCCAACATATTATTAACAATCCAACACAT1760.17600000000000002No Hit
ATACCATCCACCTTATAACTACCACTATAAATCAAC1750.17500000000000002No Hit
GTGTTGGATTGTTAATAATATGTTGGAATAGTATGA1720.172No Hit
CAATCTACATAACCAAACACCAAAAAACAACACTAA1720.172No Hit
CACAATCAAAATTAACCATCTATACAACAATATTTT1710.17099999999999999No Hit
TGGTGTTGTGTTGGAGAAATAGGGTGTGGAAATTAT1710.17099999999999999No Hit
TGTGTTGGATTGTTAATAATATGTTGGAATAGTATG1700.16999999999999998No Hit
AATATGTTGGAATAGTATGATGAAAAGTTGTAGGTA1700.16999999999999998No Hit
ATCTACTCACAATCAAAATTAACCATCTATACAACA1690.169No Hit
CCATCTATACAACAATATTTTTCATAAATCCACCCC1680.168No Hit
AATTATGGTGTTGTGTTGGAGAAATAGGGTGTGGAA1680.168No Hit
TTACCATCCACCTTATAACTACCACTATAAATCAAC1660.166No Hit
CCTATCCAAACAATCTACATAACCAAACACCAAAAA1640.164No Hit
GGGTGTGGAAATTATGTTGATTTATAGTGGTAGTTA1640.164No Hit
ATAATTTCCACACCCTATTTCTCCAACACAACACCA1620.16199999999999998No Hit
TATAAACCATCATAACACCAATAAAACCTATCCAAA1610.161No Hit
ATAGTAGGTAGTGTAGATTATTAATGGTGTGTTTAG1610.161No Hit
ATCCAACACATCTACTCACAATCAAAATTAACCATC1600.16No Hit
ATTATTAACAATCCAACACATCTACTCACAATCAAA1600.16No Hit
CCATCATAACACCAATAAAACCTATCCAAACAATCT1590.159No Hit
CTACTCACAATCAAAATTAACCATCTATACAACAAT1590.159No Hit
TGTATAGATGGTTAATTTTGATTGTGAGTAGATGTG1580.158No Hit
CAATCCAACACATCTACTCACAATCAAAATTAACCA1580.158No Hit
GTAGGTAGTGTAGATTATTAATGGTGTGTTTAGTTA1570.157No Hit
CAAACACCAAAAAACAACACTAACAAACAACTAACC1570.157No Hit
ATTAACCATCTATACAACAATATTTTTCATAAATCC1560.156No Hit
CCAATAAAACCTATCCAAACAATCTACATAACCAAA1560.156No Hit
TTTATGAAAAATATTGTTGTATAGATGGTTAATTTT1550.155No Hit
CAAAATTAACCATCTATACAACAATATTTTTCATAA1550.155No Hit
CAATAAAACCTATCCAAACAATCTACATAACCAAAC1540.154No Hit
TGGAATAGTATGATGAAAAGTTGTAGGTATAGTAGG1540.154No Hit
ATAGGGTGTGGAAATTATGTTGATTTATAGTGGTAG1540.154No Hit
ATTAATAATCTACACTACCTACTATACCTACAACTT1530.153No Hit
GATGAAAAGTTGTAGGTATAGTAGGTAGTGTAGATT1520.152No Hit
GTTAATAATATGTTGGAATAGTATGATGAAAAGTTG1510.151No Hit
CTACAACTTTTCATCATACTATTCCAACATATTATT1500.15No Hit
TAGTATGATGAAAAGTTGTAGGTATAGTAGGTAGTG1490.149No Hit
GGAATAGTATGATGAAAAGTTGTAGGTATAGTAGGT1490.149No Hit
CCATAATTACTATAAACCATCATAACACCAATAAAA1490.149No Hit
GTATAGATGGTTAATTTTGATTGTGAGTAGATGTGT1480.148No Hit
TGTGAGTAGATGTGTTGGATTGTTAATAATATGTTG1480.148No Hit
TTGGAGAAATAGGGTGTGGAAATTATGTTGATTTAT1480.148No Hit
GGTTTTATTGGTGTTATGATGGTTTATAGTAATTAT1480.148No Hit
TAATATGTTGGAATAGTATGATGAAAAGTTGTAGGT1460.146No Hit
TACCATCCACCTTATAACTACCACTATAAATCAACA1460.146No Hit
TTCCAACATATTATTAACAATCCAACACATCTACTC1450.145No Hit
GTGGATTTATGAAAAATATTGTTGTATAGATGGTTA1440.14400000000000002No Hit
ATTACTATAAACCATCATAACACCAATAAAACCTAT1440.14400000000000002No Hit
TATTATTAACAATCCAACACATCTACTCACAATCAA1420.14200000000000002No Hit
CATCATAACACCAATAAAACCTATCCAAACAATCTA1390.13899999999999998No Hit
CAACACAACACCATAATTACTATAAACCATCATAAC1380.13799999999999998No Hit
GTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTTGG1380.13799999999999998No Hit
TACAACAATATTTTTCATAAATCCACCCCATAAATC1370.13699999999999998No Hit
CAACATATTATTAACAATCCAACACATCTACTCACA1340.134No Hit
ATTTTTCATAAATCCACCCCATAAATCCAATCTATA1330.133No Hit
TAACAATCCAACACATCTACTCACAATCAAAATTAA1320.132No Hit
ATGAAAAATATTGTTGTATAGATGGTTAATTTTGAT1310.131No Hit
AATAATATGTTGGAATAGTATGATGAAAAGTTGTAG1310.131No Hit
GTTTATAGTAATTATGGTGTTGTGTTGGAGAAATAG1300.13No Hit
TGGTTAATTTTGATTGTGAGTAGATGTGTTGGATTG1300.13No Hit
TGTAGATTGTTTGGATAGGTTTTATTGGTGTTATGA1290.129No Hit
TGTTAATAATATGTTGGAATAGTATGATGAAAAGTT1280.128No Hit
GATTTATGAAAAATATTGTTGTATAGATGGTTAATT1280.128No Hit
GTGTTGTGTTGGAGAAATAGGGTGTGGAAATTATGT1280.128No Hit
CCTTATAACTACCACTATAAATCAACATAATTTCCA1270.127No Hit
TATTGTTGTATAGATGGTTAATTTTGATTGTGAGTA1270.127No Hit
AATAGGGTGTGGAAATTATGTTGATTTATAGTGGTA1270.127No Hit
CTACTATACCTACAACTTTTCATCATACTATTCCAA1270.127No Hit
CTTTTCATCATACTATTCCAACATATTATTAACAAT1260.126No Hit
ATAATCTACACTACCTACTATACCTACAACTTTTCA1250.125No Hit
CATAATTACTATAAACCATCATAACACCAATAAAAC1240.124No Hit
CATCATACTATTCCAACATATTATTAACAATCCAAC1220.122No Hit
GTGTTGTTTTTTGGTGTTTGGTTATGTAGATTGTTT1210.121No Hit
GGTTAATTTTGATTGTGAGTAGATGTGTTGGATTGT1210.121No Hit
GAGAAATAGGGTGTGGAAATTATGTTGATTTATAGT1210.121No Hit
ATTAACAATCCAACACATCTACTCACAATCAAAATT1200.12No Hit
GTTATGTAGATTGTTTGGATAGGTTTTATTGGTGTT1200.12No Hit
ATTGTTAATAATATGTTGGAATAGTATGATGAAAAG1200.12No Hit
TATGTTGGAATAGTATGATGAAAAGTTGTAGGTATA1200.12No Hit
ACTATAAATCAACATAATTTCCACACCCTATTTCTC1200.12No Hit
ATAAATCAACATAATTTCCACACCCTATTTCTCCAA1190.11900000000000001No Hit
TTAACCATCTATACAACAATATTTTTCATAAATCCA1180.11800000000000001No Hit
TGGAGAAATAGGGTGTGGAAATTATGTTGATTTATA1180.11800000000000001No Hit
CAACACCATAATTACTATAAACCATCATAACACCAA1170.117No Hit
GTGTTGGAGAAATAGGGTGTGGAAATTATGTTGATT1160.116No Hit
TTGGATTGTTAATAATATGTTGGAATAGTATGATGA1160.116No Hit
CTACCACTATAAATCAACATAATTTCCACACCCTAT1160.116No Hit
CATAAATCCACCCCATAAATCCAATCTATATTTATC1150.11499999999999999No Hit
TAAACCATCATAACACCAATAAAACCTATCCAAACA1140.11399999999999999No Hit
ACACAACACCATAATTACTATAAACCATCATAACAC1130.11299999999999999No Hit
CACCCTATTTCTCCAACACAACACCATAATTACTAT1130.11299999999999999No Hit
ACATAACCAAACACCAAAAAACAACACTAACAAACA1130.11299999999999999No Hit
TAGGGTGTGGAAATTATGTTGATTTATAGTGGTAGT1130.11299999999999999No Hit
TTGGATTTATGGGGTGGATTTATGAAAAATATTGTT1100.11No Hit
AAATTAACCATCTATACAACAATATTTTTCATAAAT1100.11No Hit
ATTCCAACATATTATTAACAATCCAACACATCTACT1100.11No Hit
GGGGTGGATTTATGAAAAATATTGTTGTATAGATGG1090.109No Hit
AATCTACATAACCAAACACCAAAAAACAACACTAAC1090.109No Hit
TCCAAACAATCTACATAACCAAACACCAAAAAACAA1080.108No Hit
TGTGTTGGAGAAATAGGGTGTGGAAATTATGTTGAT1070.107No Hit
GATTGTTAATAATATGTTGGAATAGTATGATGAAAA1070.107No Hit
GATGGTTAATTTTGATTGTGAGTAGATGTGTTGGAT1070.107No Hit
TAATTTCCACACCCTATTTCTCCAACACAACACCAT1060.106No Hit
ACATATTATTAACAATCCAACACATCTACTCACAAT1050.105No Hit
TATGAAAAATATTGTTGTATAGATGGTTAATTTTGA1050.105No Hit
ACCATAATTACTATAAACCATCATAACACCAATAAA1040.104No Hit
TAACACCAATAAAACCTATCCAAACAATCTACATAA1030.10300000000000001No Hit
ACTTTTCATCATACTATTCCAACATATTATTAACAA1030.10300000000000001No Hit
GTTGTTTGTTAGTGTTGTTTTTTGGTGTTTGGTTAT1030.10300000000000001No Hit
TTTATTGGTGTTATGATGGTTTATAGTAATTATGGT1030.10300000000000001No Hit
GGTGTTGTGTTGGAGAAATAGGGTGTGGAAATTATG1020.10200000000000001No Hit
GAGTAGATGTGTTGGATTGTTAATAATATGTTGGAA1020.10200000000000001No Hit
CACATCTACTCACAATCAAAATTAACCATCTATACA1010.101No Hit
CCAACACAACACCATAATTACTATAAACCATCATAA1010.101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)