FastQCFastQC Report
Wed 15 Sep 2021
4_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337481
Sequences flagged as poor quality0
Sequence length150
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAATAT24685973.14752534216741No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAATAT5541416.419887341805907No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTGATAT59171.7532838885744677No Hit
ATAAATCCTTTACAACTCCCTACCCGTCCATTTCATATCAATACTAATAT15790.4678781916611602No Hit
ATAAATCCTTTACAACTCCTAACCGTCCATTTCATATCAATACTAATATT11700.34668618381479255No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCATTTCATATAAATACTAATAT7800.23112412254319503No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAATAC5310.1573421911159443No Hit
ATAAATCCTTTACAACTCCCAACCGTCCATTTCATATCAATACTAATATT5020.14874911476497937No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATAATAATAT4670.13837816054829755No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCACTTCATATCAATACTAATAT4400.13037771015257155No Hit
ATAAATCCTTTACAACTCCCTAACCGTACATTTCATATCAATACTAATAT4220.1250440765554209No Hit
ATAAATCCTTTACAACTCCCTAACCGCCCATTTCATATCAATACTAATAT3970.11763625211493388No Hit
ATAAATCCTTTACAACTCCCTACCCATCCATTTCATATCAATACTAATAT3680.10904317576396892No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACCAATAT3390.100450099413004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)