FastQCFastQC Report
Wed 15 Sep 2021
4_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337481
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTAGTGAATATTTTTGTTGTTACGGTAACGATATTATGTTATGGGAG31045191.99066021494544No Hit
TTGGTAGTGAATATTTTTGTTGTTATGGTAACGATATTATGTTATGGGAG51321.5206782011431754No Hit
TTGGTAGTGAATATTTTTGTTGTTATGGTAATGATATTATGTTATGGGAG16720.4954352985797719No Hit
TTGGTAGTGAATATTTTTGTTGTTACGGTAATGATATTATGTTATGGGAG11650.34520461892669513No Hit
TTGGTAGTGAATATTTTGTTGTTACGGTAACGATATTATGTTATGGGAGG8810.2610517332827626No Hit
TTGGTAGTGAATATTTTTGTTGTTACGGTAACGATATTATGTCATGGGAG8010.2373466950732041No Hit
TTGGTAGTGAATATTTTTGTGTTACGGTAACGATATTATGTTATGGGAGG6500.1926034354526625No Hit
TTGGTAGTGAATATTTTTGTTGTTACGGTAACGATATTATGTTATGGGGG5440.16119425982499755No Hit
TTGGTAGTGAATATTTTTGTTGTTACGGTAACGATACTATGTTATGGGAG3820.11319155745064166No Hit
TTGGTAGTGAATATTTTTGTTGTTACGGTGACGATATTATGTTATGGGAG3610.10696898492063257No Hit
TTGGTAGTGAATATTTTTGTTGTTACGGTAACGATATTATGCTATGGGAG3520.10430216812205724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)