FastQCFastQC Report
Wed 15 Sep 2021
3_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90182
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAATAT6282169.66024262047858No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAATAT1271414.09815706016722No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATGCTAATAT13161.459271251469251No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATAACAACAAAAATAT9451.0478809518529195No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAATGT9101.0090705462287375No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATGCTAATAT6420.7118937260207137No Hit
ATAAATCCTTTACAACTCCCTAACCATCCACTTCATATCAATACTAATAT5420.6010068528087645No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTGATAT4980.5522166285955069No Hit
ATAAATCCTTTACAACTCCCTACCCATCCATTTCATATCAATACTAATAT3890.43134993679448225No Hit
ATAAATCCTTTACAACTCCTAACCATCCATTTCATATCAATACTAATATT3190.35372912554611785No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATCTCATATCAATACTAATAT3010.333769488367967No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAACACTAATAT2910.32268080104677205No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTCCATATCAATACTAATAT2860.31713645738617463No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATGTCAATACTAATAT2760.3060477700649797No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACCAATAT2200.2439511210662882No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATACCAATACTAATAT2010.22288261515601782No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAACAT1920.21290279656694241No Hit
ATAAATCCTTTACAACTCCCTAACCCTCCATTTCATATCAATACTAATAT1770.19626976558515005No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATAAATACTAATAT1440.1596770974252068No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCACATCAATACTAATAT1400.15524162249672882No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATAATAATAT1360.1508061475682509No Hit
ATAAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAATAC1190.13195537912221952No Hit
ATAAATCCTTTACAACTCCCAACCATCCATTTCATATCAATACTAATATT1160.12862877292586103No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCACTTCATATCAATACTAATAT1140.12641103546162205No Hit
ATAAATCCTTTACAACTCCCTAACCGTCCATTTCATAACAACAAAAATAT1020.11310461067618816No Hit
ATAAATCCTTTACAACTCCCTAACCATACATTTCATATCAATACTAATAT970.10756026701559071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)