FastQCFastQC Report
Wed 15 Sep 2021
2_S2_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_S2_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317905
Sequences flagged as poor quality0
Sequence length150
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAATATTC24845278.15290731507841No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAATATTC290489.137320897752474No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAACATTC72792.28967773391422No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATGCTAATATTC38141.1997294789323856No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAATATCC35331.1113382928862396No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAATGTTC29660.9329831238892121No Hit
AAATCCTTTACAACTCCCTACCCGTCCATTTCATATCAATACTAATATTC14080.4428996083735707No Hit
AAATCCTTTACAACTCCTAACCGTCCATTTCATATCAATACTAATATTCG12430.3909973105172929No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTGATATTC6510.20477815699658702No Hit
AAATCCTTTACAACTCCCTAACCGCCCATTTCATATCAATACTAATATTC4620.1453264339975779No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAATACTC4130.12991302433116814No Hit
AAATCCTTTACAACCCCTAACCGTCCATTTCATATCAATACTAATATTCG3470.10915210518865699No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTCCATATCAATACTAATATTC3250.10223179880781993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)