FastQCFastQC Report
Wed 15 Sep 2021
1_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181098
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAATATTC13520474.65792002120399No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAATATTC2483613.714121635799401No Hit
AAATCCTTTACAACTCCCTAACCATACACACATATAAACTACCAAAATCT13350.7371699300930987No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATGTCAATACTAATATTC9040.4991772410518063No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATGCTAATATTC8900.49144662006206585No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTGATATTC7670.42352759279506125No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAATGTTC6980.3854266750599123No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAACATTC6840.377696054070172No Hit
AAATCCTTTACAACTCCCTAACCACCCATTTCATATCAATACTAATATTC6800.3754873052159604No Hit
AAATCCTTTACAACTCCCTACCCATCCATTTCATATCAATACTAATATTC6360.35119106781963355No Hit
AAATCCTTTACAACTCCTAACCATCCATTTCATATCAATACTAATATTCA5890.32523826878264805No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAATACTC5030.27775016841710015No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACCAATATTC4160.22970988083799931No Hit
AAATCCTTTACAACTCCCTAACCATCCACTTCATATCAATACTAATATTC4060.22418800870247052No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATACCAATACTAATATTC4050.22363582148891759No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCATATCAATACTAATATCC3980.2197705109940474No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTGATATTC3820.21093551557720128No Hit
AAATCCTTTACAACTCCCTAACCATCCATTCCATATCAATACTAATATTC3260.18001303161823984No Hit
AAATCCTTTACAACTCCCTAACCATCCATTTCACATCAATACTAATATTC2810.1551646070083601No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATACTAATGTTC2590.14301648831019667No Hit
AAATCCTTTACAACTCCCTAACCGTCCATTTCATATCAATGCTAATATTC2260.12479431026295157No Hit
AAATCCTTTACAACTCCCTAACCATCCATCTCATATCAATACTAATATTC2250.12424212304939866No Hit
AAATCCTTTACAACCCCTAACCATCCATTTCATATCAATACTAATATTCA1950.10767650664281218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)