Bismark

Bismark Processing Report

SRR389222_sub3_trimmed.fq.gz

Data processed at 13:42:28 on 2021-09-13


Alignment Stats

Sequences analysed in total 79877
Single-end alignments with a unique best hit 39627
Sequences without alignments under any condition 40250
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 361611
Methylated C's in CpG context 907
Methylated C's in CHG context 939
Methylated C's in CHH context 836
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 100352
Unmethylated C's in CHG context 142664
Unmethylated C's in CHH context 115913
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 0.7%
Percentage methylation (CHH context) 0.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 39616 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 11 original bottom strand

Deduplication

Alignments analysed 39627
Unique alignments 311
Duplicates removed 39316
Duplicated alignments were found at 298 different positions

Cytosine Methylation after Extraction

Total C's analysed 2923
Methylated C's in CpG context 42
Methylated C's in CHG context 36
Methylated C's in CHH context 49
Unmethylated C's in CpG context 864
Unmethylated C's in CHG context 998
Unmethylated C's in CHH context 934
Percentage methylation (CpG context) 4.6%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 5.0%

M-Bias Plot