Bismark

Bismark Processing Report

SRR389222_sub2_trimmed.fq.gz

Data processed at 13:42:31 on 2021-09-13


Alignment Stats

Sequences analysed in total 119828
Single-end alignments with a unique best hit 59741
Sequences without alignments under any condition 60087
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 543990
Methylated C's in CpG context 1406
Methylated C's in CHG context 1376
Methylated C's in CHH context 1352
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 151362
Unmethylated C's in CHG context 214886
Unmethylated C's in CHH context 173608
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 59713 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 28 original bottom strand

Deduplication

Alignments analysed 59741
Unique alignments 323
Duplicates removed 59418
Duplicated alignments were found at 304 different positions

Cytosine Methylation after Extraction

Total C's analysed 3028
Methylated C's in CpG context 77
Methylated C's in CHG context 55
Methylated C's in CHH context 101
Unmethylated C's in CpG context 865
Unmethylated C's in CHG context 996
Unmethylated C's in CHH context 934
Percentage methylation (CpG context) 8.2%
Percentage methylation (CHG context) 5.2%
Percentage methylation (CHH context) 9.8%

M-Bias Plot