Bismark

Bismark Processing Report

SRR389222_sub1_trimmed.fq.gz

Data processed at 13:42:27 on 2021-09-13


Alignment Stats

Sequences analysed in total 99829
Single-end alignments with a unique best hit 49589
Sequences without alignments under any condition 50240
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 451198
Methylated C's in CpG context 1173
Methylated C's in CHG context 1178
Methylated C's in CHH context 1010
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 125416
Unmethylated C's in CHG context 178381
Unmethylated C's in CHH context 144040
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 0.7%
Percentage methylation (CHH context) 0.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 49575 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 14 original bottom strand

Deduplication

Alignments analysed 49589
Unique alignments 310
Duplicates removed 49279
Duplicated alignments were found at 300 different positions

Cytosine Methylation after Extraction

Total C's analysed 2899
Methylated C's in CpG context 48
Methylated C's in CHG context 29
Methylated C's in CHH context 48
Unmethylated C's in CpG context 853
Unmethylated C's in CHG context 996
Unmethylated C's in CHH context 925
Percentage methylation (CpG context) 5.3%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 4.9%

M-Bias Plot