Bismark report for: SRR389222_sub2_trimmed.fq.gz (version: v0.23.1) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /mnt/lustre/agalicina/tmp/DimaTO/methylseq/work/8d/3acbd1b2f2da033dc410392bdd58da/BismarkIndex/ with the specified options: -q --score-min L,0,-0.2 --ignore-quals Final Alignment report ====================== Sequences analysed in total: 119828 Number of alignments with a unique best hit from the different alignments: 59741 Mapping efficiency: 49.9% Sequences with no alignments under any condition: 60087 Sequences did not map uniquely: 0 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 59713 ((converted) top strand) CT/GA: 28 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 543990 Total methylated C's in CpG context: 1406 Total methylated C's in CHG context: 1376 Total methylated C's in CHH context: 1352 Total methylated C's in Unknown context: 0 Total unmethylated C's in CpG context: 151362 Total unmethylated C's in CHG context: 214886 Total unmethylated C's in CHH context: 173608 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 0.9% C methylated in CHG context: 0.6% C methylated in CHH context: 0.8% Can't determine percentage of methylated Cs in Unknown context (CN or CHN) if value was 0 Bismark completed in 0d 0h 0m 21s