/* * ------------------------------------------------- * nf-core/methylseq Nextflow config file * ------------------------------------------------- * Default config options for all environments. */ // Global default params, used in configs params { // Workflow flags genome = false input = null input_paths = null single_end = false aligner = 'bismark' clip_r1 = 0 clip_r2 = 0 three_prime_clip_r1 = 0 three_prime_clip_r2 = 0 rrbs = false pbat = false single_cell = false epignome = false accel = false zymo = false cegx = false em_seq = false comprehensive = false cytosine_report = false ignore_flags = false meth_cutoff = false methyl_kit = false min_depth = 0 skip_deduplication = false non_directional = false skip_trimming = false outdir = './results' save_align_intermeds = false known_splices = false slamseq = false local_alignment = false minins = false maxins = false save_reference = false save_trimmed = false unmapped = false relax_mismatches = false num_mismatches = 0.6 // 0.6 will allow a penalty of bp * -0.6 // For 100bp reads, this is -60. Mismatches cost -6, gap opening -5 and gap extension -2 // So -60 would allow 10 mismatches or ~ 8 x 1-2bp indels // Bismark default is 0.2 (L,0,-0.2), Bowtie2 default is 0.6 (L,0,-0.6) bismark_align_cpu_per_multicore = null bismark_align_mem_per_multicore = null publish_dir_mode = 'copy' // Boilerplate options multiqc_config = false email = false email_on_fail = false max_multiqc_email_size = 25.MB plaintext_email = false monochrome_logs = false help = false igenomes_base = 's3://ngi-igenomes/igenomes/' tracedir = "${params.outdir}/pipeline_info" igenomes_ignore = false custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" hostnames = false config_profile_name = false config_profile_description = false config_profile_contact = false config_profile_url = false validate_params = true show_hidden_params = false schema_ignore_params = 'genomes,input_paths' // Defaults only, expecting to be overwritten max_memory = 128.GB max_cpus = 16 max_time = 240.h } // Container slug. Stable releases should specify release tag! // Developmental code should specify :dev process.container = 'nfcore/methylseq:1.6.1' // Load base.config by default for all pipelines includeConfig 'conf/base.config' // Load nf-core custom profiles from different Institutions try { includeConfig "${params.custom_config_base}/nfcore_custom.config" } catch (Exception e) { System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } profiles { conda { docker.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud = false process.conda = "$projectDir/environment.yml" } debug { process.beforeScript = 'echo $HOSTNAME' } docker { docker.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false // Avoid this error: // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 // once this is established and works well, nextflow might implement this behavior as new default. docker.runOptions = '-u \$(id -u):\$(id -g)' } singularity { docker.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false singularity.autoMounts = false } podman { singularity.enabled = false docker.enabled = false podman.enabled = false shifter.enabled = false charliecloud = false } shifter { singularity.enabled = false docker.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false } charliecloud { singularity.enabled = false docker.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false } test { includeConfig 'conf/test.config' } cfdna { includeConfig 'conf/cfdna.config' } test_paired { includeConfig 'conf/test_paired.config' } test_full { includeConfig 'conf/test_full.config' } } // Load igenomes.config if required if (!params.igenomes_ignore) { includeConfig 'conf/igenomes.config' } // Export these variables to prevent local Python/R libraries from conflicting with those in the container env { PYTHONNOUSERSITE = 1 R_PROFILE_USER = "/.Rprofile" R_ENVIRON_USER = "/.Renviron" } // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] timeline { enabled = true file = "${params.tracedir}/execution_timeline.html" } report { enabled = true file = "${params.tracedir}/execution_report.html" } trace { enabled = true file = "${params.tracedir}/execution_trace.txt" } dag { enabled = true file = "${params.tracedir}/pipeline_dag.svg" } manifest { name = 'nf-core/methylseq' author = 'Phil Ewels' homePage = 'https://github.com/nf-core/methylseq' description = 'Methylation (Bisulfite-Sequencing) Best Practice analysis pipeline, part of the nf-core community.' mainScript = 'main.nf' nextflowVersion = '>=20.07.1' version = '1.6.1' } // Function to ensure that resource requirements don't go beyond // a maximum limit def check_max(obj, type) { if (type == 'memory') { try { if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) return params.max_memory as nextflow.util.MemoryUnit else return obj } catch (all) { println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" return obj } } else if (type == 'time') { try { if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) return params.max_time as nextflow.util.Duration else return obj } catch (all) { println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" return obj } } else if (type == 'cpus') { try { return Math.min( obj, params.max_cpus as int ) } catch (all) { println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" return obj } } } executor { name = 'local' cpus = 20 memory = '30 GB' }