===================== Where are my files? ===================== By default, the nf-core/methylseq pipeline does not save large intermediate files to the results directory. This is to try to conserve disk space. These files can be found in the pipeline `work` directory if needed. Alternatively, re-run the pipeline using `-resume` in addition to one of the below command-line options and they will be copied into the results directory: `--unmapped` (bismark only) Specify to save FastQ files containing reads that didn't map to the reference genome to the results directory. `--save_align_intermeds` The final BAM files created after the Bismark deduplicate / Picard MarkDuplicates steps are always saved and can be found in the `bismark_deduplicated/` or `bwa-mem_markDuplicates/` folder, respectively. Specify this flag to also copy out BAM files from bismark / bwameth alignment and sorting steps. `--save_trimmed` Specify to save trimmed FastQ files to the results directory. `--save_reference` Save any downloaded or generated reference genome files to your results folder. These can then be used for future pipeline runs, reducing processing times. ----------------------------------- Setting defaults in a config file ----------------------------------- If you would always like these files to be saved without having to specify this on the command line, you can save the following to your personal configuration file (eg. `~/.nextflow/config`): params.save_reference = true params.save_trimmed = true params.save_align_intermeds = true params.unmapped = true For more help, see the following documentation: https://github.com/nf-core/methylseq/blob/master/docs/usage.md https://www.nextflow.io/docs/latest/getstarted.html https://www.nextflow.io/docs/latest/config.html