##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	NAN20_S3_R1_001.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1020808	
Filtered Sequences	0	
Sequence length	151	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26626162804367	32.0	32.0	32.0	32.0	32.0
2	31.356583216432472	32.0	32.0	32.0	32.0	32.0
3	31.65384675668686	32.0	32.0	32.0	32.0	32.0
4	31.681785409205258	32.0	32.0	32.0	32.0	32.0
5	31.723678693740645	32.0	32.0	32.0	32.0	32.0
6	35.383894914616654	36.0	36.0	36.0	36.0	36.0
7	35.33591429534251	36.0	36.0	36.0	36.0	36.0
8	35.354489776725885	36.0	36.0	36.0	36.0	36.0
9	35.392410717784344	36.0	36.0	36.0	36.0	36.0
10-14	35.37227392418555	36.0	36.0	36.0	36.0	36.0
15-19	35.35725778011144	36.0	36.0	36.0	36.0	36.0
20-24	35.28941465975972	36.0	36.0	36.0	36.0	36.0
25-29	35.17475117749861	36.0	36.0	36.0	36.0	36.0
30-34	35.054706271894425	36.0	36.0	36.0	36.0	36.0
35-39	34.93833766976748	36.0	36.0	36.0	36.0	36.0
40-44	34.80124685543217	36.0	36.0	36.0	34.4	36.0
45-49	34.69134136879805	36.0	36.0	36.0	32.0	36.0
50-54	34.57667259660974	36.0	36.0	36.0	32.0	36.0
55-59	34.450158501892616	36.0	36.0	36.0	32.0	36.0
60-64	34.32737106292271	36.0	36.0	36.0	32.0	36.0
65-69	34.136004028181596	36.0	36.0	36.0	32.0	36.0
70-74	34.01820930086755	36.0	36.0	36.0	32.0	36.0
75-79	33.87504153572464	36.0	36.0	36.0	32.0	36.0
80-84	33.74699355804421	36.0	36.0	36.0	28.0	36.0
85-89	33.55703599501571	36.0	36.0	36.0	27.0	36.0
90-94	33.35843547464362	36.0	36.0	36.0	27.0	36.0
95-99	33.212005195884046	36.0	36.0	36.0	22.2	36.0
100-104	33.08867015148784	36.0	36.0	36.0	21.0	36.0
105-109	32.97144281784625	36.0	36.0	36.0	15.4	36.0
110-114	32.88583494643459	36.0	36.0	36.0	14.0	36.0
115-119	32.76378261142154	36.0	33.6	36.0	14.0	36.0
120-124	32.64538718348602	36.0	32.0	36.0	14.0	36.0
125-129	32.51664073949264	36.0	32.0	36.0	14.0	36.0
130-134	32.39113055540317	36.0	32.0	36.0	14.0	36.0
135-139	32.217444808426265	36.0	32.0	36.0	14.0	36.0
140-144	32.04783818308634	36.0	32.0	36.0	14.0	36.0
145-149	31.82432915886239	36.0	32.0	36.0	14.0	36.0
150-151	30.265618510043026	34.0	29.5	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	2.0
15	19.0
16	62.0
17	167.0
18	404.0
19	848.0
20	1710.0
21	3331.0
22	6093.0
23	9464.0
24	12539.0
25	14782.0
26	16873.0
27	18662.0
28	20830.0
29	23331.0
30	26487.0
31	32451.0
32	43719.0
33	71362.0
34	170821.0
35	546848.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.952824180669182	32.45960907264978	20.588742468035075	21.998824278645962
2	19.254566507510763	36.05782512181598	27.405510569140734	17.28209780153252
3	17.806957734778486	27.81791001496877	34.826977484149566	19.54815476610318
4	18.313203676727742	28.488272421896966	28.85445616619922	24.344067735176072
5	19.559855015673982	30.908503134796238	29.810834639498434	19.72080721003135
6	20.915904882278593	30.65253813820875	28.88735388926447	19.544203090248182
7	21.413659292611936	30.038276115096778	29.063494069504408	19.48457052278688
8	19.97713573672594	29.439845142127748	30.092759943848346	20.49025917729796
9	20.2883018793991	29.32411185290041	30.206356747860756	20.181229519839736
10-14	20.773428250166486	30.125076974288874	29.438533911888708	19.66296086365593
15-19	20.877305036794382	29.782623176934347	29.397653623404207	19.942418162867064
20-24	20.835463999288407	29.609301498147154	29.354424869093844	20.200809633470598
25-29	20.987844922845433	29.289993808825947	29.46667737713654	20.255483891192075
30-34	21.191760520939216	29.07383446590643	29.337783688596343	20.396621324558016
35-39	21.160348952548496	29.540470550097496	29.097292738622105	20.2018877587319
40-44	21.17694151402503	29.39126802393257	28.837273276057495	20.594517185984902
45-49	21.145190868410122	29.14808661374127	29.237427606366722	20.469294911481885
50-54	21.29758741711565	29.169688020001765	28.75404907063139	20.778675492251196
55-59	21.482075494247866	29.267353680962728	28.864768893840107	20.385801930949306
60-64	21.40001829930492	29.40535683742557	28.73116263869707	20.46346222457244
65-69	21.360606239963058	29.45201103323494	28.79420320076701	20.393179526034995
70-74	21.3505517804454	29.611299610815657	28.461321878751527	20.576826729987417
75-79	21.727531581893487	29.15169869315019	28.740350861381366	20.38041886357496
80-84	21.69820157729424	29.451337235415988	28.497529647754344	20.352931539535433
85-89	21.561804505500838	29.495317730603777	28.525305659847746	20.417572104047643
90-94	21.598924236137854	29.381349504823916	28.577801115450107	20.441925143588122
95-99	21.48799501335848	29.369240811270124	28.642179068258578	20.500585107112826
100-104	21.69977319106643	29.471713573838365	28.52974098302167	20.298772252073526
105-109	21.652875292708142	29.361160192555207	28.67536162907444	20.31060288566221
110-114	21.81682370742646	29.410233991581208	28.443260558856874	20.329681742135453
115-119	21.948567357811406	29.392323191475857	28.36052379351394	20.298585657198792
120-124	21.955488828118323	29.3582565797982	28.506110106541822	20.18014448554165
125-129	22.236091958572903	29.50631163364091	27.999699002770313	20.257897405015875
130-134	22.311406312976985	29.35573681932443	28.141895711436998	20.19096115626159
135-139	22.699827853405278	29.30916328757263	27.803517655513136	20.18749120350895
140-144	22.72393638924554	29.445266853500407	27.71166819769986	20.119128559554188
145-149	22.88498434679191	29.474051032221915	27.563307147635243	20.077657473350936
150-151	23.29326222283196	29.028616505508452	27.58115686039134	20.096964411268253
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	46.951648459315145
2	36.53666430904328
3	37.355112500881674
4	42.657271411903814
5	39.280662225705335
6	40.46010797252677
7	40.898229815398814
8	40.4673949140239
9	40.46953139923883
10-14	40.43638911382242
15-19	40.819723199661446
20-24	41.036273632759
25-29	41.243328814037504
30-34	41.58838184549723
35-39	41.362236711280396
40-44	41.77145870000993
45-49	41.61448577989201
50-54	42.07626290936685
55-59	41.86787742519717
60-64	41.863480523877364
65-69	41.75378576599805
70-74	41.92737851043282
75-79	42.10795044546845
80-84	42.05113311682967
85-89	41.97937660954848
90-94	42.04084937972598
95-99	41.988580120471305
100-104	41.99854544313996
105-109	41.96347817837036
110-114	42.146505449561914
115-119	42.247153015010205
120-124	42.13563331365997
125-129	42.49398936358878
130-134	42.50236746923858
135-139	42.88731905691423
140-144	42.84306494879973
145-149	42.96264182014284
150-151	43.390226634100216
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	40.5
2	51.5
3	65.0
4	87.0
5	120.0
6	150.5
7	162.5
8	185.5
9	260.0
10	324.5
11	390.0
12	484.5
13	576.5
14	738.0
15	985.0
16	1305.0
17	1696.0
18	2218.5
19	2915.0
20	3771.5
21	4756.5
22	5997.5
23	7583.0
24	9407.0
25	11376.5
26	13491.5
27	15937.0
28	18593.5
29	21440.5
30	24677.0
31	28224.5
32	31994.5
33	34115.0
34	34500.5
35	35392.5
36	36781.0
37	38566.0
38	39884.0
39	40610.5
40	40134.5
41	38185.0
42	36433.0
43	35056.5
44	33628.0
45	31983.5
46	30084.0
47	27988.0
48	25817.5
49	23375.0
50	20816.0
51	18389.0
52	16148.5
53	14118.5
54	12367.5
55	10776.0
56	9425.5
57	8440.0
58	7911.5
59	7771.0
60	7954.5
61	8492.0
62	9379.0
63	10372.5
64	10890.0
65	10815.5
66	9960.0
67	8338.5
68	6268.5
69	4309.5
70	2932.0
71	1999.0
72	1364.5
73	970.0
74	743.5
75	569.5
76	431.0
77	322.0
78	248.0
79	203.0
80	168.0
81	141.5
82	116.5
83	92.0
84	79.0
85	71.5
86	60.0
87	52.5
88	49.0
89	41.5
90	31.5
91	26.0
92	22.0
93	22.5
94	28.0
95	67.5
96	105.0
97	118.5
98	254.0
99	1144.5
100	1894.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015477935125900268
2	5.877696883253266E-4
3	0.0015673858355342042
4	8.8165453248799E-4
5	7.836929177671021E-4
6	0.017437167420318023
7	0.006563428186299481
8	9.796161472088776E-5
9	2.938848441626633E-4
10-14	0.001234316345483186
15-19	0.0
20-24	3.9184645888355105E-5
25-29	0.0
30-34	5.485850424369715E-4
35-39	0.03524658897657542
40-44	0.003938056911779688
45-49	0.0
50-54	0.0017829013879201575
55-59	4.3103110477190617E-4
60-64	4.114387818277286E-4
65-69	0.03209222498256283
70-74	0.005662181330867312
75-79	0.0017045320961434473
80-84	0.02202177098925557
85-89	0.04807956050501171
90-94	0.07907461540270061
95-99	0.047217498295467904
100-104	0.05544627393202248
105-109	0.04980368492409934
110-114	0.013597072123259222
115-119	8.620622095438123E-4
120-124	0.013557887477370868
125-129	0.019651099913010086
130-134	0.009580645919702823
135-139	0.05043063925831302
140-144	0.035677620081347325
145-149	0.037049082687439755
150-151	0.03237631366525341
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	1020808.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	8.958361393854267
#Duplication Level	Relative count
1	100.0
2	3.949572283777429
3	0.6081548760706115
4	0.28637701375349384
5	0.17594750106995102
6	0.10673091656495527
7	0.08665282334976566
8	0.060234279645568815
9	0.042798040800798895
10++	0.35770708175482535
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1847	0.1809351023894797	No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1477	0.14468930494275123	No Hit
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC	1070	0.1048189277513499	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	1049670	15.031044	32.73486	145-147
GATCG	425950	3.5611732	6.3403797	1
GCCCC	189710	3.4395192	4.3161464	9
GGGGC	214485	3.258	4.450054	140-144
GGCCC	187995	3.213187	3.9818816	4
CCCTG	245040	3.1514792	4.498684	1
GGGCC	192720	3.1052651	3.3652709	60-64
CCAGG	255110	3.0067134	3.6802917	8
CCAGC	206310	2.5793045	10.814294	4
TCCAG	246485	2.1859627	9.059679	3
CAGCC	147015	1.8379933	6.964515	5
GATCC	194440	1.7243994	20.646122	1
GATCT	250315	1.5747408	18.297808	1
ATCTG	226795	1.4267756	9.389806	2
ATCCA	207305	1.3447906	9.172219	2
TCTGG	152780	1.3140023	6.0680146	3
GATCA	213215	1.3039017	17.872437	1
ATCAG	197020	1.2048622	6.264903	2
>>END_MODULE