FastQCFastQC Report
Wed 14 Mar 2018
WT14_S1_R1_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename WT14_S1_R1_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 1038333
Filtered Sequences 0
Sequence length 151
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT 1713 0.16497597591524107 TruSeq Adapter, Index 8 (97% over 36bp)
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC 1656 0.15948640753977772 No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT 1411 0.13589089434699658 No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1151 0.11085075789751457 No Hit
GATCCAGCTATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT 1144 0.11017660037772083 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 947525 13.914402 36.00961 145-147
GCCCC 180650 3.2882028 4.266316 9
GGGGC 201295 3.1190228 4.3479047 140-144
GGCCC 177725 3.0658963 4.1065073 8
CCTGG 227845 2.7770457 5.0070844 4
CCAGC 207760 2.5803185 15.005232 4
GATCG 301045 2.5036044 11.134491 1
TCCAG 246680 2.164611 12.400946 3
CAGCC 153620 1.9079155 9.410215 5
TTTTA 551555 1.8011519 5.561522 8
GTTTT 377685 1.713124 5.8224 7
GATCT 268040 1.6618054 28.309978 1
GATCC 180190 1.581163 30.957466 1
TTTAT 461330 1.5065142 5.012353 9
GATCA 249375 1.4931024 24.91118 1
ATCTG 233370 1.446857 13.681579 2
AGCCA 160955 1.3639756 6.651149 6
TCTGG 154745 1.3325845 9.227099 3
ATCAG 213965 1.2810895 8.218904 2
ATCCA 201425 1.2725118 12.938552 2
ATCTC 181355 1.1863745 6.4176173 2
CTGGT 134395 1.1573409 7.8917656 4
ATCAT 254880 1.1376766 5.576122 2
ATCAC 179265 1.132515 5.4270425 2
GGTTT 183040 1.1136757 6.7382836 6
ATCGG 125460 1.043373 5.060168 2
TGGTT 169115 1.0289514 5.517692 5
ATCAA 230900 0.995321 5.866519 2
ATCCC 100250 0.928201 6.1439223 2
ATCCT 131115 0.8577182 5.3456106 2
Produced by FastQC (version 0.10.1)