##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	WT14_S1_R1_001.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1038333	
Filtered Sequences	0	
Sequence length	151	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285914056473214	32.0	32.0	32.0	32.0	32.0
2	31.33194456884256	32.0	32.0	32.0	32.0	32.0
3	31.67335334618085	32.0	32.0	32.0	32.0	32.0
4	31.693446129517216	32.0	32.0	32.0	32.0	32.0
5	31.73199060417034	32.0	32.0	32.0	32.0	32.0
6	35.40346979244616	36.0	36.0	36.0	36.0	36.0
7	35.34844890800928	36.0	36.0	36.0	36.0	36.0
8	35.36279305386615	36.0	36.0	36.0	36.0	36.0
9	35.4035217988834	36.0	36.0	36.0	36.0	36.0
10-14	35.38335235420621	36.0	36.0	36.0	36.0	36.0
15-19	35.364564547211735	36.0	36.0	36.0	36.0	36.0
20-24	35.29457139472596	36.0	36.0	36.0	36.0	36.0
25-29	35.17608859585509	36.0	36.0	36.0	36.0	36.0
30-34	35.0531577056686	36.0	36.0	36.0	36.0	36.0
35-39	34.930328709575825	36.0	36.0	36.0	36.0	36.0
40-44	34.786465035783316	36.0	36.0	36.0	33.6	36.0
45-49	34.68032143830544	36.0	36.0	36.0	32.0	36.0
50-54	34.56136037282837	36.0	36.0	36.0	32.0	36.0
55-59	34.4372038642709	36.0	36.0	36.0	32.0	36.0
60-64	34.30363419057277	36.0	36.0	36.0	32.0	36.0
65-69	34.116725559141436	36.0	36.0	36.0	32.0	36.0
70-74	33.98669001177849	36.0	36.0	36.0	32.0	36.0
75-79	33.834449641877896	36.0	36.0	36.0	32.0	36.0
80-84	33.7167557999216	36.0	36.0	36.0	27.0	36.0
85-89	33.53077846894975	36.0	36.0	36.0	27.0	36.0
90-94	33.331415644114166	36.0	36.0	36.0	24.6	36.0
95-99	33.18313103792329	36.0	36.0	36.0	21.0	36.0
100-104	33.06318705078236	36.0	36.0	36.0	21.0	36.0
105-109	32.94874284068791	36.0	36.0	36.0	14.0	36.0
110-114	32.870893441699344	36.0	36.0	36.0	14.0	36.0
115-119	32.74090238873271	36.0	33.6	36.0	14.0	36.0
120-124	32.62785484040283	36.0	32.0	36.0	14.0	36.0
125-129	32.499590401152616	36.0	32.0	36.0	14.0	36.0
130-134	32.36913860967532	36.0	32.0	36.0	14.0	36.0
135-139	32.19360937194522	36.0	32.0	36.0	14.0	36.0
140-144	32.030154680627504	36.0	32.0	36.0	14.0	36.0
145-149	31.81341246016451	36.0	32.0	36.0	14.0	36.0
150-151	30.26634133750926	34.0	29.5	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	13.0
16	63.0
17	126.0
18	366.0
19	815.0
20	1660.0
21	3520.0
22	6253.0
23	9843.0
24	13379.0
25	15790.0
26	17597.0
27	18965.0
28	21466.0
29	23754.0
30	26623.0
31	32703.0
32	44278.0
33	71490.0
34	172167.0
35	557457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.05477240612722	32.004060925440214	18.04636351639926	21.894803152033305
2	18.534045633023123	38.258467708149745	26.333611665689133	16.873874993138
3	17.267714829171986	26.78795174921147	36.66260563888956	19.28172778272699
4	17.740932003752206	27.60646504648847	27.80987015588613	26.842732793873193
5	19.403189193301866	30.902545364189614	29.646091422770844	20.048174019737672
6	21.179980272334262	30.70907857929982	28.507343931816255	19.603597216549662
7	21.579935854835448	29.965712194323253	28.89701137469059	19.55734057615071
8	19.657853780308344	29.361145996255516	30.236784522809746	20.74421570062639
9	20.319628326612612	29.050948108069583	30.266456457526548	20.362967107791253
10-14	20.614492641960087	30.21480853686725	29.38096155327837	19.789737267894292
15-19	20.72337101873869	29.87136111440164	29.360677162336167	20.0445907045235
20-24	20.65657163934884	29.72530007232747	29.417614580293606	20.200513708030083
25-29	20.769502654736005	29.286019032429866	29.683348212952875	20.261130099881253
30-34	21.05815666441689	29.07700314660207	29.50249555438444	20.362344634596596
35-39	20.865711444933446	29.900071680167716	29.127351764642405	20.10686511025643
40-44	20.976967987872413	29.611073549495615	28.79371353393162	20.61824492870035
45-49	20.833879080002095	29.277453240425423	29.46556248632423	20.423105193248254
50-54	21.1165641831978	29.28721548341166	28.68273541197276	20.913484921417783
55-59	21.2484219828717	29.46944338611946	28.981486011554725	20.300648619454115
60-64	21.22204623460004	29.681529177701204	28.75613990380328	20.340284683895476
65-69	21.028091946528306	29.940041072193107	28.821172565254805	20.21069441602378
70-74	21.025457980520777	30.08790729551961	28.371195470643823	20.515439253315787
75-79	21.579801281961146	29.422562739606867	28.78506858320699	20.212567395224994
80-84	21.54282870617529	29.766443520915892	28.464656152158597	20.226071620750226
85-89	21.26093902666993	29.874368615086944	28.523469885276327	20.3412224729668
90-94	21.420417041935526	29.56653362614586	28.647516902520575	20.365532429398048
95-99	21.263738508524852	29.576316653639267	28.76464811380241	20.395296724033468
100-104	21.503707695308314	29.701380007354487	28.63850606745315	20.15640622988405
105-109	21.442832590532362	29.529663024160335	28.851448413715534	20.176055971591765
110-114	21.627239492941868	29.590058723208006	28.594482372538582	20.188219411311543
115-119	21.73916812093335	29.573004187517576	28.50480196932749	20.183025722221583
120-124	21.742500015411455	29.58837544770277	28.689444540337945	19.979679996547834
125-129	22.06106967292388	29.682834109994303	28.09066627416196	20.16542994291985
130-134	22.150012261160207	29.574688958541095	28.246476866860366	20.02882191343833
135-139	22.6501843259091	29.4458351248153	27.752453455033983	20.15152709424162
140-144	22.711722665515666	29.749310652466715	27.559661559746345	19.979305122271274
145-149	22.73858893977214	29.79046018061306	27.47373719038904	19.997213689225767
150-151	23.084726657899683	29.203553037857	27.63320279194786	20.078517512295456
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	49.949575558160525
2	35.40792062616112
3	36.549442611898975
4	44.5836647976254
5	39.451363213039535
6	40.78357748888392
7	41.137276430986155
8	40.402069480934735
9	40.682595434403865
10-14	40.40422990985438
15-19	40.76796172326219
20-24	40.85708534737893
25-29	41.03063275461726
30-34	41.42050129901349
35-39	40.972576555189875
40-44	41.59521291657276
45-49	41.256984273250346
50-54	42.03004910461558
55-59	41.54907060232581
60-64	41.56233091849552
65-69	41.23878636255209
70-74	41.54089723383657
75-79	41.79236867718614
80-84	41.76890032692551
85-89	41.60216149963673
90-94	41.78594947133357
95-99	41.65903523255832
100-104	41.66011392519236
105-109	41.61888856212413
110-114	41.81545890425341
115-119	41.92219384315493
120-124	41.72218001195929
125-129	42.226499615843736
130-134	42.178834174598535
135-139	42.80171142015072
140-144	42.691027787786936
145-149	42.7358026289979
150-151	43.163244170195135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	45.0
2	59.0
3	73.5
4	94.5
5	119.5
6	146.5
7	188.5
8	241.5
9	284.5
10	324.0
11	382.0
12	486.0
13	631.0
14	823.5
15	1022.0
16	1314.5
17	1772.5
18	2297.0
19	2931.5
20	3762.0
21	4804.5
22	6118.0
23	7622.0
24	9394.5
25	11431.0
26	13631.0
27	16121.0
28	18761.5
29	21477.0
30	24977.0
31	29006.5
32	33247.0
33	35883.0
34	35826.5
35	36116.0
36	38028.0
37	40248.5
38	42065.0
39	43275.0
40	42233.5
41	39831.0
42	37890.5
43	35852.0
44	33889.5
45	31885.0
46	29623.0
47	27253.5
48	24877.0
49	22442.5
50	19909.0
51	17618.5
52	15581.5
53	14006.0
54	12648.0
55	11064.0
56	9748.5
57	8860.0
58	9138.5
59	8992.0
60	8277.0
61	8608.5
62	9287.5
63	10170.5
64	10694.0
65	10467.0
66	9558.5
67	8001.0
68	6140.0
69	4440.5
70	3022.0
71	2072.0
72	1433.5
73	1008.0
74	819.5
75	659.5
76	490.5
77	383.5
78	308.0
79	252.5
80	206.5
81	176.0
82	145.5
83	114.5
84	100.5
85	80.5
86	66.5
87	54.5
88	45.0
89	41.0
90	34.0
91	25.0
92	25.5
93	25.0
94	28.0
95	56.5
96	90.0
97	104.5
98	172.0
99	625.0
100	1069.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01406099969855528
2	5.77849302680354E-4
3	7.704657369071386E-4
4	0.0010593903882473157
5	0.0013483150395874927
6	0.018972718771338288
7	0.006067417678143717
8	4.8154108556696165E-4
9	1.9261643422678466E-4
10-14	0.0012520068224741003
15-19	0.0
20-24	0.0
25-29	0.0
30-34	6.356342329483894E-4
35-39	0.03746389645710962
40-44	0.003756020467422301
45-49	1.9261643422678466E-5
50-54	0.0018491177685771329
55-59	4.430177987216047E-4
60-64	2.311397210721416E-4
65-69	0.03426646364894499
70-74	0.00552809166230872
75-79	0.001695024621195705
80-84	0.02284430909929666
85-89	0.051524896155664905
90-94	0.08230500234510509
95-99	0.050099534542386685
100-104	0.057727145337767356
105-109	0.05291173448209775
110-114	0.013521673682720284
115-119	8.667739540205309E-4
120-124	0.013887644907751174
125-129	0.02059069681884328
130-134	0.009168542269194951
135-139	0.053123612559747214
140-144	0.03804174575978997
145-149	0.03865811834931568
150-151	0.03351525955546053
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	1038333.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	10.584138857932714
#Duplication Level	Relative count
1	100.0
2	5.02974089051501
3	0.7189164901469928
4	0.31185142392536963
5	0.18454329546183965
6	0.11981941502449879
7	0.09360891798788969
8	0.06632860474570469
9	0.054025718381582044
10++	0.4027858013993196
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT	1713	0.16497597591524107	TruSeq Adapter, Index 8 (97% over 36bp)
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC	1656	0.15948640753977772	No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT	1411	0.13589089434699658	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1151	0.11085075789751457	No Hit
GATCCAGCTATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT	1144	0.11017660037772083	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	947525	13.914402	36.00961	145-147
GCCCC	180650	3.2882028	4.266316	9
GGGGC	201295	3.1190228	4.3479047	140-144
GGCCC	177725	3.0658963	4.1065073	8
CCTGG	227845	2.7770457	5.0070844	4
CCAGC	207760	2.5803185	15.005232	4
GATCG	301045	2.5036044	11.134491	1
TCCAG	246680	2.164611	12.400946	3
CAGCC	153620	1.9079155	9.410215	5
TTTTA	551555	1.8011519	5.561522	8
GTTTT	377685	1.713124	5.8224	7
GATCT	268040	1.6618054	28.309978	1
GATCC	180190	1.581163	30.957466	1
TTTAT	461330	1.5065142	5.012353	9
GATCA	249375	1.4931024	24.91118	1
ATCTG	233370	1.446857	13.681579	2
AGCCA	160955	1.3639756	6.651149	6
TCTGG	154745	1.3325845	9.227099	3
ATCAG	213965	1.2810895	8.218904	2
ATCCA	201425	1.2725118	12.938552	2
ATCTC	181355	1.1863745	6.4176173	2
CTGGT	134395	1.1573409	7.8917656	4
ATCAT	254880	1.1376766	5.576122	2
ATCAC	179265	1.132515	5.4270425	2
GGTTT	183040	1.1136757	6.7382836	6
ATCGG	125460	1.043373	5.060168	2
TGGTT	169115	1.0289514	5.517692	5
ATCAA	230900	0.995321	5.866519	2
ATCCC	100250	0.928201	6.1439223	2
ATCCT	131115	0.8577182	5.3456106	2
>>END_MODULE