FastQCFastQC Report
Wed 14 Mar 2018
SOX19_S6_R1_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename SOX19_S6_R1_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 574409
Filtered Sequences 0
Sequence length 151
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC 671 0.11681571841666825 No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT 607 0.10567383171224684 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 370195 10.571435 25.29324 145-147
GATCG 255515 3.9159124 7.138773 1
CGATC 210750 3.366895 3.9436312 50-54
GCCCC 90400 3.0482702 3.7904797 9
CCAGC 111940 2.5716922 13.338852 4
TCCAG 130670 2.0875547 10.931141 3
CAGCC 83905 1.9276208 8.1382475 5
GATCC 104320 1.666593 24.050364 1
GATCT 143580 1.595086 20.625912 1
ATCTG 130950 1.4547744 10.196645 2
AGCCA 87810 1.3744421 5.556175 6
TCTGG 86040 1.3458494 6.748202 3
ATCCA 114395 1.2979654 10.514192 2
GATCA 117805 1.282255 19.51274 1
ATCAG 112435 1.223805 6.2629237 2
CTGGT 73735 1.1533728 5.472183 4
ATCAA 126480 0.9777474 5.055912 2
Produced by FastQC (version 0.10.1)