##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SOX19_S6_R1_001.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	574409	
Filtered Sequences	0	
Sequence length	151	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23132645902136	32.0	32.0	32.0	32.0	32.0
2	31.311991977841572	32.0	32.0	32.0	32.0	32.0
3	31.65298767951059	32.0	32.0	32.0	32.0	32.0
4	31.68460278303439	32.0	32.0	32.0	32.0	32.0
5	31.722711517403106	32.0	32.0	32.0	32.0	32.0
6	35.38077223720381	36.0	36.0	36.0	36.0	36.0
7	35.34104967018274	36.0	36.0	36.0	36.0	36.0
8	35.34302561415298	36.0	36.0	36.0	36.0	36.0
9	35.38645634034285	36.0	36.0	36.0	36.0	36.0
10-14	35.367624810892586	36.0	36.0	36.0	36.0	36.0
15-19	35.351872272196296	36.0	36.0	36.0	36.0	36.0
20-24	35.29307235784955	36.0	36.0	36.0	36.0	36.0
25-29	35.18857573610441	36.0	36.0	36.0	36.0	36.0
30-34	35.07839222574855	36.0	36.0	36.0	36.0	36.0
35-39	34.9741219235771	36.0	36.0	36.0	36.0	36.0
40-44	34.8521645726303	36.0	36.0	36.0	35.2	36.0
45-49	34.75325839253911	36.0	36.0	36.0	33.6	36.0
50-54	34.64861170350743	36.0	36.0	36.0	32.0	36.0
55-59	34.537497845611746	36.0	36.0	36.0	32.0	36.0
60-64	34.42189102190251	36.0	36.0	36.0	32.0	36.0
65-69	34.242766043011166	36.0	36.0	36.0	32.0	36.0
70-74	34.129241707563764	36.0	36.0	36.0	32.0	36.0
75-79	33.99644887179693	36.0	36.0	36.0	32.0	36.0
80-84	33.878994583998505	36.0	36.0	36.0	31.0	36.0
85-89	33.701222299789876	36.0	36.0	36.0	27.0	36.0
90-94	33.49796886887218	36.0	36.0	36.0	27.0	36.0
95-99	33.36600749640065	36.0	36.0	36.0	27.0	36.0
100-104	33.2508101370278	36.0	36.0	36.0	27.0	36.0
105-109	33.14864565144348	36.0	36.0	36.0	22.2	36.0
110-114	33.06825867979088	36.0	36.0	36.0	21.0	36.0
115-119	32.945525226798324	36.0	36.0	36.0	15.4	36.0
120-124	32.8355168529741	36.0	33.6	36.0	14.0	36.0
125-129	32.70487370497328	36.0	32.0	36.0	14.0	36.0
130-134	32.57867965160713	36.0	32.0	36.0	14.0	36.0
135-139	32.4113588053112	36.0	32.0	36.0	14.0	36.0
140-144	32.25068548717029	36.0	32.0	36.0	14.0	36.0
145-149	32.034421118053515	36.0	32.0	36.0	14.0	36.0
150-151	30.468770510211364	34.0	29.5	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	4.0
16	28.0
17	74.0
18	177.0
19	408.0
20	786.0
21	1601.0
22	2910.0
23	4471.0
24	6040.0
25	7224.0
26	8313.0
27	9535.0
28	11146.0
29	12621.0
30	14618.0
31	18167.0
32	25457.0
33	41933.0
34	101191.0
35	307701.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.42612389873594	31.68645749904238	22.381690287982728	21.505728314238954
2	18.955026662279533	36.61010398550157	27.454400799433152	16.980468552785744
3	17.179168008635024	28.016991495547565	35.60083912918585	19.203001366631558
4	17.6043133779829	28.65360141643701	28.977244041163026	24.76484116441707
5	19.16507514811133	30.93633519556963	30.316034672762314	19.582554983556726
6	20.500064427828644	30.84821848347339	29.368293846619974	19.283423242077987
7	21.312280298558917	30.16615043901114	29.40861029425847	19.112958968171476
8	19.65122291336979	29.669206677495225	30.409796537994836	20.269773871140153
9	19.915007851589294	29.546871028505972	30.649575387443722	19.88854573246101
10-14	20.387924250801877	30.37346048264557	29.707061689329123	19.53155357722343
15-19	20.462353479837535	30.070872844958906	29.696470633294396	19.770303041909163
20-24	20.4189697584822	29.81353008048272	29.66729281748719	20.10020734354789
25-29	20.581606485970795	29.48501851468205	29.825368335106173	20.108006664240985
30-34	20.78701292189759	29.294497539017776	29.646305184079242	20.27218435500539
35-39	20.76050636332889	29.84628878091667	29.3377031547217	20.05550170103274
40-44	20.810204191066735	29.63354106906952	29.07189550676586	20.484359233097884
45-49	20.744695852606768	29.38380143765157	29.511689405980757	20.35981330376091
50-54	20.919107431731813	29.378840118078735	29.009652567460382	20.69239988272907
55-59	21.0466885954146	29.474423830678592	29.188458751635167	20.290428822271643
60-64	21.007125609541667	29.616665535064858	29.019980606085927	20.35622824930755
65-69	20.944483996672968	29.6852555948601	29.0827188031604	20.28754160530653
70-74	20.934113037622335	29.851963432732397	28.739060218158418	20.47486331148685
75-79	21.355513548276125	29.353768416243938	29.012924492398824	20.277793543081117
80-84	21.33445615912085	29.675651710864003	28.764365744479818	20.22552638553533
85-89	21.098097851552225	29.688858108246986	28.816777338789773	20.39626670141101
90-94	21.19529687271308	29.526063047553997	28.8918238906042	20.386816189128723
95-99	21.09868847581778	29.460922348445923	28.984660040822487	20.455729134913813
100-104	21.26091788634598	29.58676649397796	28.871612276111474	20.28070334356459
105-109	21.20824784935729	29.47497945423337	29.069951759689605	20.246820936719736
110-114	21.359528599431748	29.56630994496821	28.794757510848434	20.27940394475161
115-119	21.41435539176771	29.53494558510979	28.77749644151409	20.27320258160841
120-124	21.45666412222247	29.536818293253692	28.88600007800486	20.12051750651898
125-129	21.6350513826724	29.688033277193394	28.445919685059113	20.230995655075095
130-134	21.77147714329192	29.522843127456916	28.600057317186554	20.105622412064605
135-139	22.114410155701574	29.439034680206117	28.285582597989777	20.160972566102533
140-144	22.12859190522717	29.6740529422317	28.17802300003657	20.01933215250456
145-149	22.211389182356285	29.695939909023366	28.12375581143823	19.968915097182123
150-151	22.504321173095093	29.294636520769927	28.104387351262396	20.096654954872587
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	45.931852212974896
2	35.93549521506528
3	36.382169375266585
4	42.36915454239997
5	38.74763013166805
6	39.783487669906634
7	40.42523926673039
8	39.92099678450994
9	39.803553584050306
10-14	39.91947782802531
15-19	40.232656521746705
20-24	40.519177102030085
25-29	40.689613150211784
30-34	41.05919727690298
35-39	40.81600806436163
40-44	41.294563424164615
45-49	41.104509156367676
50-54	41.61150731446088
55-59	41.337117417686244
60-64	41.36335385884921
65-69	41.2320256019795
70-74	41.40897634910918
75-79	41.633307091357246
80-84	41.55998254465618
85-89	41.49436455296323
90-94	41.5821130618418
95-99	41.5544176107316
100-104	41.54162122991057
105-109	41.45506878607703
110-114	41.638932544183355
115-119	41.68755797337612
120-124	41.57718162874145
125-129	41.86604703774749
130-134	41.87709955535653
135-139	42.27538272180411
140-144	42.14792405773173
145-149	42.180304279538404
150-151	42.60097612796768
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	28.5
2	34.5
3	40.5
4	44.5
5	57.0
6	79.0
7	104.5
8	127.0
9	146.5
10	178.0
11	214.0
12	269.5
13	346.5
14	447.5
15	582.0
16	776.0
17	1028.5
18	1312.0
19	1671.0
20	2143.5
21	2757.5
22	3538.0
23	4435.0
24	5399.5
25	6513.0
26	7858.0
27	9330.5
28	10737.5
29	12294.0
30	14147.0
31	16163.5
32	18345.5
33	19577.5
34	19729.0
35	19991.0
36	20772.0
37	21830.0
38	22669.5
39	23225.5
40	22767.5
41	21631.5
42	20752.0
43	20163.0
44	19390.0
45	18356.0
46	17259.0
47	15866.0
48	14705.0
49	13654.5
50	12209.5
51	10696.5
52	9451.5
53	8358.5
54	7322.0
55	6339.5
56	5441.0
57	4815.5
58	4471.0
59	4324.0
60	4246.0
61	4221.5
62	4447.5
63	4817.0
64	5038.5
65	4898.5
66	4433.5
67	3735.5
68	2887.0
69	2086.0
70	1426.0
71	951.0
72	656.0
73	480.5
74	383.5
75	307.5
76	231.5
77	189.0
78	146.5
79	104.0
80	80.0
81	72.0
82	70.0
83	57.5
84	47.5
85	34.0
86	22.5
87	21.5
88	19.5
89	17.0
90	16.5
91	13.0
92	11.5
93	8.0
94	7.5
95	29.5
96	43.0
97	39.5
98	89.0
99	359.0
100	512.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012012346603204337
2	3.481839595131692E-4
3	6.963679190263384E-4
4	0.001740919797565846
5	0.001740919797565846
6	0.021413313510059907
7	0.008704598987829229
8	3.481839595131692E-4
9	5.222759392697538E-4
10-14	0.0014275542340039938
15-19	0.0
20-24	0.0
25-29	0.0
30-34	7.311863149776554E-4
35-39	0.039762608176403924
40-44	0.004421936285817249
45-49	0.0
50-54	0.0021935589449329656
55-59	5.570943352210707E-4
60-64	3.481839595131692E-4
65-69	0.03541030868248931
70-74	0.006511040042896265
75-79	0.0019498301732737474
80-84	0.024999608293045546
85-89	0.05410778730834649
90-94	0.0858273460199962
95-99	0.054595244851664934
100-104	0.06110628489456119
105-109	0.05654507502493867
110-114	0.015041547050968909
115-119	0.001288280650198726
120-124	0.014937091863114958
125-129	0.02259713897240468
130-134	0.010654429161102976
135-139	0.05633616464923078
140-144	0.04108570722255397
145-149	0.04171243834967767
150-151	0.0350795339209518
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	574409.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	7.152063410842996
#Duplication Level	Relative count
1	100.0
2	3.047712136258
3	0.36705642582704673
4	0.14713451630177654
5	0.09982271070650563
6	0.05095117525644558
7	0.037953426466535996
8	0.029114957289397475
9	0.022876037870240874
10++	0.17936893330075232
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC	671	0.11681571841666825	No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT	607	0.10567383171224684	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	370195	10.571435	25.29324	145-147
GATCG	255515	3.9159124	7.138773	1
CGATC	210750	3.366895	3.9436312	50-54
GCCCC	90400	3.0482702	3.7904797	9
CCAGC	111940	2.5716922	13.338852	4
TCCAG	130670	2.0875547	10.931141	3
CAGCC	83905	1.9276208	8.1382475	5
GATCC	104320	1.666593	24.050364	1
GATCT	143580	1.595086	20.625912	1
ATCTG	130950	1.4547744	10.196645	2
AGCCA	87810	1.3744421	5.556175	6
TCTGG	86040	1.3458494	6.748202	3
ATCCA	114395	1.2979654	10.514192	2
GATCA	117805	1.282255	19.51274	1
ATCAG	112435	1.223805	6.2629237	2
CTGGT	73735	1.1533728	5.472183	4
ATCAA	126480	0.9777474	5.055912	2
>>END_MODULE