FastQCFastQC Report
Wed 14 Mar 2018
SOX18_S5_R2_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename SOX18_S5_R2_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 754700
Filtered Sequences 0
Sequence length 151
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1283 0.17000132502981316 No Hit
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC 1200 0.15900357758049558 No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT 1186 0.15714853584205646 No Hit
GATCCAGCTATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT 975 0.12919040678415264 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 685800 13.205257 34.17288 145-147
AAAAA 804175 3.1815584 4.2258124 145-147
GCCCC 124575 3.0646746 4.317182 9
GGGGC 149610 3.0627463 4.1093903 140-144
CCTGG 169070 2.788919 5.3300524 4
CCAGC 152985 2.579582 17.694656 4
ATCTC 253950 2.3135746 8.164064 2
GATCT 267280 2.2903438 32.177734 1
TCCAG 163635 1.9669508 14.879388 3
CAGCC 112450 1.8960941 11.168093 5
TTTTA 389410 1.8029183 5.7797637 8
CAGCT 143380 1.7234786 5.331216 5
GTTTT 269195 1.7103617 6.0429125 7
GATCG 134605 1.5218697 11.687004 1
TTTAT 328100 1.5190609 5.429038 9
ATCAT 232680 1.4529185 5.4717464 2
GATCA 174880 1.4408025 25.185228 1
ATCTG 159405 1.3659543 14.728083 2
AGCCA 117760 1.3609595 7.774965 6
TCTGG 112785 1.3262872 9.252169 3
ATCCA 135345 1.1855164 15.306009 2
CTGGT 98245 1.155305 7.68574 4
GGTTT 135305 1.1342711 6.7479076 6
ATCAG 137640 1.1339893 7.6555934 2
TGGTT 124535 1.0439855 5.829416 5
ATCAC 118715 1.0398506 5.8983192 2
GCCAT 83695 1.0060437 5.1291924 7
ATCAA 154380 0.926837 6.1435447 2
GATCC 75700 0.9099409 36.31172 1
ATCTT 130470 0.84734994 5.8343277 2
ATCCC 63955 0.8173214 6.435413 2
ATCCT 84205 0.7671374 6.436149 2
GATTA 107250 0.62990993 5.9414773 1
Produced by FastQC (version 0.10.1)