##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SOX18_S5_R2_001.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	754700	
Filtered Sequences	0	
Sequence length	151	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32201007022658	32.0	32.0	32.0	32.0	32.0
2	31.154758182059098	32.0	32.0	32.0	32.0	32.0
3	31.250014575327945	32.0	32.0	32.0	32.0	32.0
4	31.210215979859548	32.0	32.0	32.0	32.0	32.0
5	31.24676560222605	32.0	32.0	32.0	32.0	32.0
6	34.826198489466016	36.0	36.0	36.0	32.0	36.0
7	34.77809195706904	36.0	36.0	36.0	32.0	36.0
8	34.68865244468	36.0	36.0	36.0	32.0	36.0
9	34.711547634821784	36.0	36.0	36.0	32.0	36.0
10-14	34.67331071949119	36.0	36.0	36.0	32.0	36.0
15-19	34.653769179806545	36.0	36.0	36.0	32.0	36.0
20-24	34.55848973101895	36.0	36.0	36.0	32.0	36.0
25-29	34.372774877434736	36.0	36.0	36.0	32.0	36.0
30-34	34.33015449847622	36.0	36.0	36.0	32.0	36.0
35-39	34.165448257585794	36.0	36.0	36.0	32.0	36.0
40-44	34.0561049423612	36.0	36.0	36.0	32.0	36.0
45-49	33.934078706770904	36.0	36.0	36.0	32.0	36.0
50-54	33.78797561945144	36.0	36.0	36.0	31.0	36.0
55-59	33.58183198621969	36.0	36.0	36.0	27.0	36.0
60-64	33.6068284086392	36.0	36.0	36.0	27.0	36.0
65-69	33.482586458195314	36.0	36.0	36.0	22.2	36.0
70-74	33.222008215184836	36.0	36.0	36.0	16.8	36.0
75-79	33.081870147078305	36.0	36.0	36.0	14.0	36.0
80-84	32.96833549754869	36.0	36.0	36.0	14.0	36.0
85-89	32.82411819265933	36.0	36.0	36.0	14.0	36.0
90-94	32.68020060951371	36.0	34.4	36.0	14.0	36.0
95-99	32.547983039618394	36.0	32.0	36.0	14.0	36.0
100-104	32.430049821120974	36.0	32.0	36.0	14.0	36.0
105-109	32.319002782562606	36.0	32.0	36.0	14.0	36.0
110-114	32.18582191599311	36.0	32.0	36.0	14.0	36.0
115-119	32.05972280376309	36.0	32.0	36.0	14.0	36.0
120-124	31.94776440969922	36.0	32.0	36.0	14.0	36.0
125-129	31.778385583675636	36.0	32.0	36.0	14.0	36.0
130-134	31.629224592553335	36.0	32.0	36.0	14.0	36.0
135-139	31.409962634159267	36.0	32.0	36.0	14.0	36.0
140-144	31.117424142043195	36.0	32.0	36.0	14.0	36.0
145-149	30.788993507353915	36.0	27.0	36.0	14.0	36.0
150-151	28.582824301046774	31.5	20.5	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	85.0
4	57.0
5	49.0
6	37.0
7	46.0
8	68.0
9	95.0
10	217.0
11	180.0
12	97.0
13	59.0
14	253.0
15	1061.0
16	1344.0
17	1431.0
18	1547.0
19	1859.0
20	2516.0
21	3553.0
22	5945.0
23	8898.0
24	11757.0
25	14282.0
26	16088.0
27	17999.0
28	19829.0
29	21868.0
30	25151.0
31	30646.0
32	42508.0
33	69653.0
34	154323.0
35	301199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.700005314343414	30.388478503480894	19.123664771217516	20.787851410958176
2	19.272418796622162	39.512759819611475	24.9333897987821	16.28143158498426
3	17.800841544494144	25.99183924696094	37.72716660564674	18.48015260289818
4	18.18853155605288	26.39630048484543	27.771951618869327	27.643216340232357
5	19.746428434157146	30.28677231706784	29.751716697525914	20.215082551249104
6	21.852773779322373	29.993054616691715	28.166047679261137	19.988123924724768
7	22.49390276231377	29.009198875987487	28.534409628333595	19.962488733365145
8	20.313361465711015	28.819606429449934	29.770001908477884	21.097030196361167
9	20.590726135112103	28.686372558244944	30.124090657880608	20.598810648762342
10-14	20.91314089038089	29.697477049142083	29.313266221469153	20.07611583900787
15-19	20.937734841265527	29.253637181759025	29.255916840181367	20.552711136794084
20-24	20.994345232414872	29.17398297949948	29.182227085626305	20.64944470245934
25-29	21.01964678940188	28.877911850588323	29.469017586297035	20.63342377371276
30-34	21.453914904243547	28.58934927179923	29.222273415807436	20.734462408149785
35-39	21.204405008390616	29.573364227068954	28.731439236281403	20.49079152825902
40-44	21.44319862082684	29.226668434837382	28.39202997049365	20.938102973842124
45-49	21.151105248244413	28.98810540849453	29.146206476439517	20.71458286682154
50-54	21.54990485707864	29.00448741303332	28.393110224885437	21.0524975050026
55-59	21.434286822863687	29.54297735759825	28.51800906524091	20.50472675429715
60-64	21.138333299272052	29.957993151824752	28.344868606177904	20.558804942725292
65-69	20.870786546674836	30.175629781953027	28.5473903687339	20.406193302638243
70-74	21.06583040485111	30.08658186741135	28.143923942355897	20.703663785381643
75-79	21.689421606930527	29.345883939188038	28.58530979276372	20.379384661117715
80-84	21.556940425099327	29.979485303817366	28.193122148838118	20.270452122245185
85-89	21.112111112909993	30.117886676233773	28.34190294473841	20.42809926611782
90-94	21.224668770988806	29.96217104040787	28.456603929837527	20.3565562587658
95-99	21.085047483369472	29.901184370105106	28.555612094436388	20.458156052089034
100-104	21.348757164894224	30.071720604425643	28.419667720897447	20.159854509782686
105-109	21.282098175738252	29.842354926637483	28.70052904041979	20.175017857204477
110-114	21.477460210432426	29.95557478686649	28.416606826908907	20.150358175792174
115-119	21.647505917056094	29.854959124795993	28.37035939811143	20.127175560036484
120-124	21.70838795850973	29.808052947689102	28.65891354140759	19.824645552393587
125-129	22.169171588067165	29.919876317044817	27.916107321084848	19.99484477380317
130-134	22.32252522536321	29.64011700119534	28.272421977303214	19.76493579613824
135-139	23.06589934641876	29.523647229108057	27.712051583626575	19.69840184084661
140-144	23.208536390164294	29.786237199754833	27.60778326046337	19.3974431496175
145-149	23.489933598486378	29.781461088118437	27.48103193091015	19.247573382485033
150-151	23.961732787783795	29.0557551450515	27.721698069386363	19.260813997778342
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	50.4878567253016
2	35.55385038160642
3	36.28099414739233
4	45.831747896285236
5	39.96151098540625
6	41.840897704047144
7	42.456391495678915
8	41.41039166207218
9	41.189536783874445
10-14	40.989256729388764
15-19	41.49044597805961
20-24	41.64378993487421
25-29	41.65307056311464
30-34	42.188377312393335
35-39	41.69519653664964
40-44	42.38130159466896
45-49	41.86568811506595
50-54	42.60240236208124
55-59	41.939013577160836
60-64	41.697138241997344
65-69	41.27697984931308
70-74	41.769494190232756
75-79	42.06880626804824
80-84	41.827392547344516
85-89	41.54021037902781
90-94	41.581225029754606
95-99	41.5432035354585
100-104	41.50861167467691
105-109	41.45711603294273
110-114	41.627818386224604
115-119	41.77468147709258
120-124	41.53303351090331
125-129	42.16401636187033
130-134	42.087461021501454
135-139	42.764301187265374
140-144	42.6059795397818
145-149	42.73750698097141
150-151	43.22254678556214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66.0
1	63.5
2	93.0
3	116.5
4	128.0
5	154.5
6	174.0
7	190.0
8	216.5
9	274.5
10	325.5
11	372.5
12	443.0
13	527.0
14	663.0
15	851.0
16	1042.5
17	1327.0
18	1724.5
19	2193.5
20	2801.5
21	3538.5
22	4451.5
23	5567.0
24	6836.5
25	8243.5
26	9803.5
27	11490.0
28	13176.0
29	15151.5
30	17398.5
31	19896.0
32	22769.5
33	24447.0
34	24256.0
35	24339.0
36	25233.5
37	26496.0
38	27858.5
39	28604.0
40	27954.5
41	26469.5
42	25625.5
43	25237.0
44	24775.0
45	24116.0
46	23174.5
47	22068.0
48	20893.5
49	19372.5
50	17520.5
51	15511.5
52	13786.0
53	12434.0
54	11065.0
55	9783.5
56	8759.0
57	7959.0
58	7512.5
59	7334.5
60	7430.5
61	7666.0
62	7978.5
63	8205.5
64	8000.0
65	7353.0
66	6092.0
67	4541.0
68	3116.0
69	1952.5
70	1158.5
71	711.5
72	497.5
73	405.0
74	393.5
75	362.0
76	293.5
77	245.5
78	223.5
79	200.0
80	174.5
81	161.0
82	156.5
83	147.5
84	143.5
85	147.0
86	146.5
87	137.0
88	121.0
89	106.5
90	92.5
91	89.0
92	90.5
93	92.0
94	103.0
95	99.5
96	98.5
97	104.0
98	102.5
99	136.0
100	443.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2676560222605009
2	0.19040678415264345
3	0.11236252815688354
4	0.058168808798197955
5	0.02636809328209885
6	0.032065721478733274
7	0.0344507751424407
8	0.022790512786537695
9	0.02265800980522062
10-14	0.028938651119650195
15-19	0.026633099244733005
20-24	0.02936266065986485
25-29	0.042029945673777665
30-34	0.03513979064528952
35-39	0.03789585265668477
40-44	0.08281436332317477
45-49	0.11639061878892275
50-54	0.13141645687027959
55-59	0.14524976811978269
60-64	0.15319994699880748
65-69	0.1507353915463098
70-74	0.14895985159666092
75-79	0.1542334702530807
80-84	0.15786405194116868
85-89	0.15182191599310985
90-94	0.1594275871207102
95-99	0.16380018550417383
100-104	0.15433947263813436
105-109	0.16199814495826156
110-114	0.17212137273088646
115-119	0.1702398303961839
120-124	0.1632171723863787
125-129	0.17090234530276932
130-134	0.16851729163906187
135-139	0.16173313899562738
140-144	0.16660924870809593
145-149	0.1620776467470518
150-151	0.16019610441234927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	754700.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	8.542907062373956
#Duplication Level	Relative count
1	100.0
2	3.8712936941488523
3	0.5268032881787658
4	0.2341932699695812
5	0.13472691485890514
6	0.08209921374214532
7	0.06683718041828497
8	0.046838653993916235
9	0.026313850558379908
10++	0.26629616765080466
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1283	0.17000132502981316	No Hit
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC	1200	0.15900357758049558	No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT	1186	0.15714853584205646	No Hit
GATCCAGCTATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT	975	0.12919040678415264	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	685800	13.205257	34.17288	145-147
AAAAA	804175	3.1815584	4.2258124	145-147
GCCCC	124575	3.0646746	4.317182	9
GGGGC	149610	3.0627463	4.1093903	140-144
CCTGG	169070	2.788919	5.3300524	4
CCAGC	152985	2.579582	17.694656	4
ATCTC	253950	2.3135746	8.164064	2
GATCT	267280	2.2903438	32.177734	1
TCCAG	163635	1.9669508	14.879388	3
CAGCC	112450	1.8960941	11.168093	5
TTTTA	389410	1.8029183	5.7797637	8
CAGCT	143380	1.7234786	5.331216	5
GTTTT	269195	1.7103617	6.0429125	7
GATCG	134605	1.5218697	11.687004	1
TTTAT	328100	1.5190609	5.429038	9
ATCAT	232680	1.4529185	5.4717464	2
GATCA	174880	1.4408025	25.185228	1
ATCTG	159405	1.3659543	14.728083	2
AGCCA	117760	1.3609595	7.774965	6
TCTGG	112785	1.3262872	9.252169	3
ATCCA	135345	1.1855164	15.306009	2
CTGGT	98245	1.155305	7.68574	4
GGTTT	135305	1.1342711	6.7479076	6
ATCAG	137640	1.1339893	7.6555934	2
TGGTT	124535	1.0439855	5.829416	5
ATCAC	118715	1.0398506	5.8983192	2
GCCAT	83695	1.0060437	5.1291924	7
ATCAA	154380	0.926837	6.1435447	2
GATCC	75700	0.9099409	36.31172	1
ATCTT	130470	0.84734994	5.8343277	2
ATCCC	63955	0.8173214	6.435413	2
ATCCT	84205	0.7671374	6.436149	2
GATTA	107250	0.62990993	5.9414773	1
>>END_MODULE