##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SOX18_S5_R1_001.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	754700	
Filtered Sequences	0	
Sequence length	151	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22171326354843	32.0	32.0	32.0	32.0	32.0
2	31.21913078044256	32.0	32.0	32.0	32.0	32.0
3	31.647326089837023	32.0	32.0	32.0	32.0	32.0
4	31.6781184576653	32.0	32.0	32.0	32.0	32.0
5	31.712893865111965	32.0	32.0	32.0	32.0	32.0
6	35.37108519941699	36.0	36.0	36.0	36.0	36.0
7	35.32162846164039	36.0	36.0	36.0	36.0	36.0
8	35.32975751954419	36.0	36.0	36.0	36.0	36.0
9	35.36877567245263	36.0	36.0	36.0	36.0	36.0
10-14	35.350960646614546	36.0	36.0	36.0	36.0	36.0
15-19	35.33103378826024	36.0	36.0	36.0	36.0	36.0
20-24	35.268576387968736	36.0	36.0	36.0	36.0	36.0
25-29	35.14675076189214	36.0	36.0	36.0	36.0	36.0
30-34	35.01712071021598	36.0	36.0	36.0	36.0	36.0
35-39	34.900228700145746	36.0	36.0	36.0	36.0	36.0
40-44	34.75463177421492	36.0	36.0	36.0	32.8	36.0
45-49	34.64321028223135	36.0	36.0	36.0	32.0	36.0
50-54	34.515082019345435	36.0	36.0	36.0	32.0	36.0
55-59	34.38051252153174	36.0	36.0	36.0	32.0	36.0
60-64	34.25447065058964	36.0	36.0	36.0	32.0	36.0
65-69	34.05960408109183	36.0	36.0	36.0	32.0	36.0
70-74	33.916316417119376	36.0	36.0	36.0	32.0	36.0
75-79	33.76976175963959	36.0	36.0	36.0	27.0	36.0
80-84	33.64031906717901	36.0	36.0	36.0	27.0	36.0
85-89	33.438597058433814	36.0	36.0	36.0	25.8	36.0
90-94	33.22968278786273	36.0	36.0	36.0	21.0	36.0
95-99	33.08004743606731	36.0	36.0	36.0	21.0	36.0
100-104	32.95230581688088	36.0	36.0	36.0	15.4	36.0
105-109	32.83409672717636	36.0	36.0	36.0	14.0	36.0
110-114	32.743661587385716	36.0	34.4	36.0	14.0	36.0
115-119	32.612382933616004	36.0	32.0	36.0	14.0	36.0
120-124	32.492527626871606	36.0	32.0	36.0	14.0	36.0
125-129	32.36473327149861	36.0	32.0	36.0	14.0	36.0
130-134	32.23181820590963	36.0	32.0	36.0	14.0	36.0
135-139	32.06201113025043	36.0	32.0	36.0	14.0	36.0
140-144	31.89872717636147	36.0	32.0	36.0	14.0	36.0
145-149	31.67496329667417	36.0	32.0	36.0	14.0	36.0
150-151	30.115315357095536	34.0	29.5	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	4.0
15	6.0
16	21.0
17	94.0
18	290.0
19	567.0
20	1172.0
21	2352.0
22	4492.0
23	7195.0
24	9836.0
25	11702.0
26	13285.0
27	14549.0
28	16450.0
29	18418.0
30	20523.0
31	25125.0
32	34102.0
33	55849.0
34	133295.0
35	385372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.319389760518636	30.661459416980634	19.782292082179836	21.23685874032089
2	18.569380437634326	38.571261994927745	26.21950088380191	16.63985668363602
3	17.179413113129293	26.541883200343452	37.36706532713921	18.911638359388043
4	17.59554572666164	27.232602482340457	27.633031267101373	27.538820523896533
5	19.477717944369065	30.3137190468114	29.949860074626866	20.258702934192673
6	21.29178042250908	30.519786890025713	28.521483287830996	19.666949399634216
7	22.07048434800123	29.581641631242512	28.749085685815146	19.598788334941116
8	20.117875120743822	29.098167873994463	29.92313471499158	20.860822290270136
9	20.295773546804753	28.879195546833902	30.396489196355102	20.42854171000624
10-14	20.805803280391537	30.009550936383977	29.346338578044715	19.838307205179767
15-19	20.950502438188007	29.653751067643054	29.230350928938897	20.165395565230043
20-24	20.89890022525507	29.462488405989134	29.290049026103087	20.34856234265271
25-29	21.072373128395387	28.97930303431827	29.594275871207103	20.354047966079236
30-34	21.395451142514528	28.704417016145594	29.395557145601863	20.50457469573801
35-39	21.193567226502015	29.64083071566368	28.886745679685248	20.278856378149058
40-44	21.294219849566513	29.301561843258085	28.586125582438154	20.81809272473725
45-49	21.173081005189083	28.985299850351094	29.18726094799548	20.654358196464344
50-54	21.422665484367307	28.99000858103406	28.471780906430688	21.115545028167944
55-59	21.592120595216674	29.20145658533509	28.691026301165262	20.515396518282977
60-64	21.531588901847705	29.393164370518242	28.46805333943908	20.60719338819497
65-69	21.421338232214385	29.534182154606352	28.59483231670948	20.449647296469784
70-74	21.408798890935742	29.69858247658331	28.109748809454715	20.78286982302623
75-79	21.918475828244468	28.98201717110122	28.557152068156505	20.54235493249781
80-84	21.883734707428303	29.438553383351024	28.15044537482125	20.527266534399423
85-89	21.535682555186405	29.513269420796945	28.270943975913358	20.68010404810329
90-94	21.74193363592531	29.21474783147427	28.370782493938524	20.6725360386619
95-99	21.57021979927428	29.126890320952224	28.518466670590612	20.784423209182883
100-104	21.880408281772226	29.23205216079376	28.3546626398845	20.532876917549515
105-109	21.736329170543236	29.0859369883622	28.62109312547046	20.556640715624106
110-114	21.97691230199801	29.152943078527592	28.32479681315026	20.545347806324138
115-119	22.045414493045516	29.122305361252852	28.24841359334193	20.5838665523597
120-124	22.092922786273565	29.05516114309583	28.497750585611065	20.35416548501954
125-129	22.430280595191963	29.19682800364507	27.822822830782506	20.550068570380457
130-134	22.448334863445446	28.99428169187288	28.117506249430186	20.439877195251494
135-139	23.00663833018567	28.82658492552593	27.61820573388189	20.548571010406512
140-144	23.094931650113264	29.117303595974164	27.477356987488516	20.310407766424056
145-149	23.080934354915634	29.170768098149104	27.429437041911086	20.31886050502418
150-151	23.589762281881864	28.5161670852856	27.511879278694174	20.382191354138364
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	49.55624850083953
2	35.20923712127034
3	36.091051472517336
4	45.13436625055818
5	39.73642087856174
6	40.95872982214329
7	41.66927268294234
8	40.97869741101396
9	40.724315256810996
10-14	40.6441104855713
15-19	41.11589800341805
20-24	41.247462567907775
25-29	41.42642109447463
30-34	41.90002583825254
35-39	41.47242360465107
40-44	42.112312574303765
45-49	41.827439201653426
50-54	42.53821051253525
55-59	42.10751711349965
60-64	42.13878229004268
65-69	41.870985528684166
70-74	42.191668713961974
75-79	42.460830760742276
80-84	42.41100124182773
85-89	42.21578660328969
90-94	42.41446967458721
95-99	42.35464300845717
100-104	42.413285199321734
105-109	42.29296988616734
110-114	42.52226010832214
115-119	42.629281045405214
120-124	42.447088271293104
125-129	42.98034916557242
130-134	42.888212058696936
135-139	43.555209340592185
140-144	43.405339416537316
145-149	43.39979485993982
150-151	43.97195363602023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	35.5
2	51.5
3	52.5
4	63.0
5	89.0
6	115.0
7	136.0
8	156.0
9	187.5
10	233.5
11	286.0
12	352.0
13	468.0
14	591.0
15	766.5
16	999.5
17	1312.5
18	1709.0
19	2162.0
20	2750.0
21	3529.5
22	4541.5
23	5683.5
24	7036.5
25	8450.5
26	9915.0
27	11694.5
28	13566.5
29	15552.5
30	17896.0
31	20408.5
32	23142.5
33	24795.5
34	24708.5
35	24778.0
36	25656.0
37	26834.5
38	28165.5
39	29073.5
40	28316.0
41	26629.0
42	25620.5
43	25103.0
44	24418.0
45	23454.5
46	22451.0
47	21331.5
48	20101.5
49	18635.0
50	16792.5
51	14900.0
52	13196.0
53	11710.5
54	10322.0
55	8941.0
56	7776.5
57	6899.0
58	6262.5
59	5921.0
60	5899.0
61	6451.0
62	7074.5
63	7519.5
64	8134.0
65	8248.0
66	7576.0
67	6564.5
68	5128.5
69	3651.5
70	2582.0
71	1730.0
72	1156.5
73	835.5
74	634.0
75	501.5
76	374.5
77	278.0
78	199.5
79	137.5
80	109.5
81	97.0
82	86.0
83	69.5
84	57.5
85	41.0
86	34.5
87	33.0
88	23.5
89	19.5
90	16.5
91	13.0
92	13.5
93	14.5
94	19.5
95	41.5
96	68.0
97	70.0
98	112.5
99	484.0
100	1133.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014707830926195839
2	7.950178879024777E-4
3	7.950178879024777E-4
4	0.0011925268318537167
5	0.0015900357758049555
6	0.021200477010732743
7	0.004770107327414867
8	3.975089439512389E-4
9	1.3250298131707965E-4
10-14	0.0012720286206439645
15-19	2.6500596263415924E-5
20-24	0.0
25-29	0.0
30-34	6.625149065853982E-4
35-39	0.03654432224725056
40-44	0.0036570822843513978
45-49	2.6500596263415924E-5
50-54	0.0016430369683317874
55-59	4.5051013647807076E-4
60-64	1.8550417384391148E-4
65-69	0.032065721478733274
70-74	0.0060686365443222475
75-79	0.001775539949648867
80-84	0.0221279978799523
85-89	0.0472505631376706
90-94	0.07860076851729164
95-99	0.047330064926460844
100-104	0.05525374320922221
105-109	0.050642639459387837
110-114	0.013117795150390885
115-119	9.275208692195575E-4
120-124	0.01372730886444945
125-129	0.01945143765734729
130-134	0.00943421226977607
135-139	0.05085464422949516
140-144	0.036173313899562735
145-149	0.03824036040810918
150-151	0.031138200609513715
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	754700.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	9.66377113681021
#Duplication Level	Relative count
1	100.0
2	4.350679247879169
3	0.5503839457236755
4	0.23020866960557582
5	0.14077127842547854
6	0.10372620515561577
7	0.06403505522361994
8	0.05768447123450061
9	0.047100164585968385
10++	0.29847744748860866
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1450	0.19212932290976545	No Hit
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC	1269	0.16814628329137404	No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT	1177	0.15595600901020273	No Hit
GATCCAGCTATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT	1118	0.14813833311249502	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	716900	13.367759	32.134014	145-147
GCCCC	143395	3.3545196	4.297267	9
GGGGC	159650	3.1505983	4.4899774	140-144
GGCCC	141415	3.125847	3.6388369	5
CCCTG	182590	3.076215	4.296524	4
GGGCC	144555	3.0191267	3.3701587	75-79
CCTGG	181970	2.8967793	5.054142	4
CCAGC	159380	2.6158383	16.332167	4
TCCAG	178965	2.1153803	13.916438	3
GATCG	172690	1.928695	11.678394	1
CAGCC	114655	1.8817853	9.721973	5
TTTTA	391675	1.8302077	5.7883344	8
CAGCT	146890	1.7362516	5.4292865	5
GTTTT	272920	1.7175332	5.8307385	7
GATCT	192290	1.6368937	32.50861	1
TTTAT	331405	1.5485798	5.658323	9
GATCC	130860	1.5467757	35.541676	1
ATCTG	167860	1.4289299	15.139243	2
AGCCA	117245	1.350057	7.0322666	6
GATCA	160175	1.328299	25.593655	1
TCTGG	114960	1.3179706	9.209251	3
ATCCA	146845	1.2887964	14.918523	2
ATCAG	148495	1.2314389	7.392425	2
ATCTC	128165	1.1546683	7.819208	2
CTGGT	100135	1.1480079	7.6758313	4
ATCAC	127850	1.1220853	5.8435717	2
GGTTT	134280	1.108699	6.60795	6
ATCAT	175400	1.1086594	5.6159067	2
TGGTT	124990	1.0319949	5.54614	5
ATCAA	162965	1.0034604	6.7288666	2
ATCTT	139910	0.9077785	5.8601413	2
ATCCC	71715	0.89712816	6.65592	2
ATCCT	95800	0.86308455	6.197103	2
>>END_MODULE