FastQCFastQC Report
Wed 14 Mar 2018
NAN21-22_S4_R2_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename NAN21-22_S4_R2_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 294324
Filtered Sequences 0
Sequence length 151
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC 346 0.1175575216428154 No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT 341 0.11585871352658975 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 205040 10.998021 27.921062 145-147
GATCG 99435 2.9467678 7.8091803 1
CCAGC 57600 2.5468855 13.897175 4
ATCTC 89210 2.0476377 5.5699043 2
GATCT 92515 2.0235503 22.249777 1
CAGCC 43555 1.9258611 8.279859 5
TCCAG 61510 1.9128904 11.21929 3
GATCA 71160 1.5043201 19.279308 1
ATCTG 63135 1.3809311 11.065843 2
AGCCA 44985 1.3521183 5.9387956 6
TCTGG 43645 1.3382542 7.8626523 3
ATCCA 55875 1.2395375 11.125528 2
CTGGT 37870 1.1611798 6.2820897 4
GGTTT 53085 1.14481 5.2747874 6
ATCAG 53885 1.1391273 6.2025666 2
GATCC 35920 1.1170709 24.378658 1
ATCAA 60750 0.9161115 5.1009855 2
Produced by FastQC (version 0.10.1)