##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	NAN21-22_S4_R2_001.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	294324	
Filtered Sequences	0	
Sequence length	151	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29589160245172	32.0	32.0	32.0	32.0	32.0
2	31.16326225520175	32.0	32.0	32.0	32.0	32.0
3	31.24449925931966	32.0	32.0	32.0	32.0	32.0
4	31.209459643114393	32.0	32.0	32.0	32.0	32.0
5	31.2385772142265	32.0	32.0	32.0	32.0	32.0
6	34.853280738234055	36.0	36.0	36.0	32.0	36.0
7	34.79488251043068	36.0	36.0	36.0	32.0	36.0
8	34.7199514820402	36.0	36.0	36.0	32.0	36.0
9	34.72646131474158	36.0	36.0	36.0	32.0	36.0
10-14	34.69701553390142	36.0	36.0	36.0	32.0	36.0
15-19	34.68785759910846	36.0	36.0	36.0	32.0	36.0
20-24	34.61571193650535	36.0	36.0	36.0	32.0	36.0
25-29	34.439129666625895	36.0	36.0	36.0	32.0	36.0
30-34	34.40692638045148	36.0	36.0	36.0	32.0	36.0
35-39	34.25353623897473	36.0	36.0	36.0	32.0	36.0
40-44	34.17044957257988	36.0	36.0	36.0	32.0	36.0
45-49	34.06277639608051	36.0	36.0	36.0	32.0	36.0
50-54	33.93357660265558	36.0	36.0	36.0	32.0	36.0
55-59	33.74796007121404	36.0	36.0	36.0	28.0	36.0
60-64	33.79000149495114	36.0	36.0	36.0	27.0	36.0
65-69	33.674667373370845	36.0	36.0	36.0	27.0	36.0
70-74	33.42615077261793	36.0	36.0	36.0	22.2	36.0
75-79	33.30819369130619	36.0	36.0	36.0	21.0	36.0
80-84	33.20565091531781	36.0	36.0	36.0	21.0	36.0
85-89	33.07084505510933	36.0	36.0	36.0	21.0	36.0
90-94	32.95372446691401	36.0	36.0	36.0	16.8	36.0
95-99	32.82011456761936	36.0	36.0	36.0	14.0	36.0
100-104	32.723850586428554	36.0	35.2	36.0	14.0	36.0
105-109	32.615130944129604	36.0	32.0	36.0	14.0	36.0
110-114	32.48272583955097	36.0	32.0	36.0	14.0	36.0
115-119	32.376915915793475	36.0	32.0	36.0	14.0	36.0
120-124	32.25444747964828	36.0	32.0	36.0	14.0	36.0
125-129	32.09203870564412	36.0	32.0	36.0	14.0	36.0
130-134	31.950631277095987	36.0	32.0	36.0	14.0	36.0
135-139	31.72848629401612	36.0	32.0	36.0	14.0	36.0
140-144	31.427163941778446	36.0	32.0	36.0	14.0	36.0
145-149	31.11065424498172	36.0	31.0	36.0	14.0	36.0
150-151	28.873290999035078	34.0	20.5	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	39.0
4	21.0
5	22.0
6	15.0
7	15.0
8	27.0
9	36.0
10	92.0
11	63.0
12	32.0
13	20.0
14	40.0
15	179.0
16	291.0
17	364.0
18	537.0
19	655.0
20	936.0
21	1368.0
22	2021.0
23	3058.0
24	4075.0
25	4931.0
26	5521.0
27	6432.0
28	7236.0
29	8309.0
30	9374.0
31	12045.0
32	16353.0
33	26632.0
34	60037.0
35	123547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.535451581360963	32.129070944046774	19.75687618606331	21.578601288528958
2	19.57605857703554	37.428982833333905	26.178910497094638	16.816048092535922
3	18.0319671824456	27.33367574977295	35.60371306409424	19.030644003687215
4	18.49343708886192	27.803558012326917	28.45355988210217	25.249445016708993
5	19.698421760939603	30.781099193888235	29.71976401179941	19.800715033372757
6	21.031577444779405	30.444653654126547	28.607989015358786	19.91577988573526
7	21.703958750412124	29.64593197352902	28.961384593945123	19.688724682113737
8	20.037654287423194	29.101870480126145	29.966083954107987	20.894391278342667
9	20.28023122644491	29.118423690855273	30.3054812629776	20.295863819722214
10-14	20.58288538871261	29.991081891617792	29.492497097536376	19.93353562213322
15-19	20.60525401759224	29.62846405390911	29.386079687768696	20.380202240729954
20-24	20.6346778688636	29.412972252810743	29.417458569544706	20.534891308780946
25-29	20.6976092806087	29.22917213369972	29.57255846269523	20.500660122996347
30-34	21.083096542629125	28.92057427977105	29.374328715348113	20.622000462251712
35-39	20.91239476603798	29.727098696947944	29.01024331614784	20.350263220866236
40-44	21.037168021219436	29.49073349882681	28.69065188560547	20.781446594348285
45-49	20.945071631795294	29.209090822503637	29.23113346821981	20.61470407748126
50-54	21.185252824087996	29.173193039563767	28.763783631516564	20.877770504831673
55-59	21.054332862191295	29.588650127017807	28.846890632666405	20.51012637812449
60-64	20.91669717877555	29.950890250326335	28.605544447181835	20.52686812371628
65-69	20.758235266895873	30.038137704061217	28.80431083998225	20.39931618906066
70-74	20.853840458198132	30.04379995208955	28.523255624033624	20.579103965678694
75-79	21.374951849326163	29.37599449006565	28.882849920985254	20.366203739622936
80-84	21.207309603212416	29.875518954604235	28.63043626216566	20.286735180017697
85-89	20.958986895188733	30.038437876007904	28.619475916438454	20.383099312364912
90-94	20.998318893055735	29.799492264866227	28.819141478421255	20.383047363656782
95-99	20.880331060018243	29.966104463592924	28.75893331155307	20.394631164835765
100-104	21.10686586504564	30.078347248726654	28.61825180555291	20.1965350806748
105-109	21.047956827216986	29.92862388455677	28.842702341792698	20.18071694643354
110-114	21.174257197952805	29.976387433949135	28.704821142810076	20.144534225287977
115-119	21.309062889691187	29.896767571158634	28.665956033304475	20.128213505845707
120-124	21.418055565954198	29.804677210662334	28.824694104106356	19.95257311927711
125-129	21.791964015868302	29.977836650121297	28.23281319345555	19.997386140554845
130-134	21.95447348824672	29.898197699033847	28.4031550817427	19.744173730976733
135-139	22.50187854268648	29.748152766358295	28.045485235743495	19.70448345521173
140-144	22.69840978131175	29.901801630717774	27.950834791640716	19.448953796329764
145-149	22.908540216780956	29.946808691657168	27.813098742534333	19.331552349027547
150-151	23.263664965653604	29.44176256489402	27.975170540881315	19.319401928571065
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	48.11405286988992
2	36.39210666957146
3	37.06261118613281
4	43.742882105570914
5	39.499136794312356
6	40.94735733051466
7	41.39268343252586
8	40.93204556576586
9	40.576095046167126
10-14	40.51642101084583
15-19	40.98545625832219
20-24	41.16956917764455
25-29	41.198269403605046
30-34	41.705097004880834
35-39	41.262657986904216
40-44	41.818614615567725
45-49	41.55977570927655
50-54	42.06302332891967
55-59	41.564459240315784
60-64	41.443565302491834
65-69	41.15755145595653
70-74	41.432944423876826
75-79	41.7411555889491
80-84	41.49404478323011
85-89	41.34208620755364
90-94	41.38136625671252
95-99	41.27496222485401
100-104	41.30340094572043
105-109	41.22867377365053
110-114	41.31879142324078
115-119	41.4372763955369
120-124	41.3706286852313
125-129	41.78935015642315
130-134	41.69864721922345
135-139	42.20636199789821
140-144	42.147363577641514
145-149	42.24009256580851
150-151	42.583066894224665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	29.5
2	37.0
3	43.5
4	48.0
5	53.0
6	57.0
7	65.0
8	84.0
9	100.5
10	121.0
11	140.5
12	155.0
13	191.5
14	265.5
15	332.0
16	402.5
17	507.0
18	639.0
19	811.0
20	1062.0
21	1371.0
22	1744.0
23	2137.5
24	2578.0
25	3167.5
26	3785.0
27	4507.0
28	5248.0
29	6028.0
30	6894.0
31	7856.0
32	8838.0
33	9525.5
34	9804.0
35	9912.5
36	10300.5
37	10763.0
38	11213.5
39	11618.0
40	11458.5
41	10940.5
42	10548.0
43	10278.5
44	10078.5
45	9748.0
46	9293.0
47	8762.0
48	8074.0
49	7385.0
50	6577.5
51	5774.5
52	5123.0
53	4494.0
54	4019.5
55	3565.0
56	3167.0
57	2875.0
58	2728.0
59	2697.5
60	2664.0
61	2699.5
62	2829.0
63	2936.0
64	2758.5
65	2470.0
66	2100.5
67	1569.5
68	1075.5
69	686.0
70	404.0
71	253.5
72	196.0
73	151.0
74	130.5
75	116.5
76	81.5
77	65.5
78	64.5
79	63.5
80	56.0
81	45.0
82	48.0
83	46.5
84	36.0
85	46.0
86	49.5
87	35.5
88	32.0
89	32.5
90	33.0
91	31.5
92	28.5
93	29.0
94	34.0
95	37.5
96	37.0
97	42.0
98	41.0
99	44.0
100	109.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2755466764518014
2	0.18924722414753808
3	0.11382014378711895
4	0.05809923757491744
5	0.024462836873649446
6	0.032277354208287465
7	0.03907258667319009
8	0.023103790380668923
9	0.022764028757423792
10-14	0.030986260039955964
15-19	0.02738478683355758
20-24	0.032617115831532595
25-29	0.0463434854106359
30-34	0.038053301803454696
35-39	0.04158682268520406
40-44	0.08629945230426332
45-49	0.11837294953860372
50-54	0.13196341446840898
55-59	0.1454859270735652
60-64	0.15343634905750125
65-69	0.15003873282504995
70-74	0.15031054212364606
75-79	0.15452358625188567
80-84	0.1576493931857409
85-89	0.15194139791522268
90-94	0.15968796292521167
95-99	0.16308557915766297
100-104	0.1538440630053954
105-109	0.16043543849635095
110-114	0.16899743140212825
115-119	0.1704244302197578
120-124	0.16383305472880227
125-129	0.17178347671273833
130-134	0.16974490697326755
135-139	0.16281376985906687
140-144	0.16655114771476331
145-149	0.16274581753441786
150-151	0.16291569834604042
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	294324.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	6.218772176280608
#Duplication Level	Relative count
1	100.0
2	2.4826911692790774
3	0.20487478444392968
4	0.0761845932103055
5	0.047357990373973695
6	0.026767559776593827
7	0.021105191362314365
8	0.017501866007772888
9	0.016472344477903893
10++	0.05919748796746712
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC	346	0.1175575216428154	No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT	341	0.11585871352658975	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	205040	10.998021	27.921062	145-147
GATCG	99435	2.9467678	7.8091803	1
CCAGC	57600	2.5468855	13.897175	4
ATCTC	89210	2.0476377	5.5699043	2
GATCT	92515	2.0235503	22.249777	1
CAGCC	43555	1.9258611	8.279859	5
TCCAG	61510	1.9128904	11.21929	3
GATCA	71160	1.5043201	19.279308	1
ATCTG	63135	1.3809311	11.065843	2
AGCCA	44985	1.3521183	5.9387956	6
TCTGG	43645	1.3382542	7.8626523	3
ATCCA	55875	1.2395375	11.125528	2
CTGGT	37870	1.1611798	6.2820897	4
GGTTT	53085	1.14481	5.2747874	6
ATCAG	53885	1.1391273	6.2025666	2
GATCC	35920	1.1170709	24.378658	1
ATCAA	60750	0.9161115	5.1009855	2
>>END_MODULE