FastQCFastQC Report
Wed 14 Mar 2018
NAN21-22_S4_R1_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename NAN21-22_S4_R1_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 294324
Filtered Sequences 0
Sequence length 151
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 522 0.17735556733395852 No Hit
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC 350 0.11891656813579593 No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 333 0.11314062054062869 No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT 314 0.1066851496989712 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 258235 13.3426485 27.289558 145-147
GATCG 111835 3.2728577 6.946312 1
GCCCC 51625 3.2360926 4.098945 9
GGGGC 56200 3.047521 4.17873 140-144
GGCCC 50555 3.0195446 3.5998313 4
CCTGG 65295 2.767748 5.1095357 4
CCAGC 59305 2.56658 11.069646 4
TCCAG 69935 2.1479661 9.278083 3
CAGCC 43080 1.8644005 6.488441 5
GATCC 54845 1.6844956 22.028505 1
GATCT 74055 1.6141968 21.736237 1
ATCTG 65635 1.4306638 11.229665 2
TCTGG 44595 1.3415353 6.9648232 3
ATCCA 59530 1.324814 9.322675 2
GATCA 60430 1.2814101 18.68345 1
ATCAG 56470 1.1974388 5.984301 2
CTGGT 37950 1.1416361 5.5498414 4
ATCAA 64890 0.9970099 5.2620416 2
Produced by FastQC (version 0.10.1)