##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	NAN21-22_S4_R1_001.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	294324	
Filtered Sequences	0	
Sequence length	151	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251270708470937	32.0	32.0	32.0	32.0	32.0
2	31.31981421834441	32.0	32.0	32.0	32.0	32.0
3	31.643824492735895	32.0	32.0	32.0	32.0	32.0
4	31.669870618773867	32.0	32.0	32.0	32.0	32.0
5	31.707234204482134	32.0	32.0	32.0	32.0	32.0
6	35.36048368464685	36.0	36.0	36.0	36.0	36.0
7	35.304987021105994	36.0	36.0	36.0	36.0	36.0
8	35.32179842622416	36.0	36.0	36.0	36.0	36.0
9	35.3633682608282	36.0	36.0	36.0	36.0	36.0
10-14	35.34635775539881	36.0	36.0	36.0	36.0	36.0
15-19	35.33339245185578	36.0	36.0	36.0	36.0	36.0
20-24	35.271013576874466	36.0	36.0	36.0	36.0	36.0
25-29	35.16344436743181	36.0	36.0	36.0	36.0	36.0
30-34	35.05382571587774	36.0	36.0	36.0	36.0	36.0
35-39	34.93983297318601	36.0	36.0	36.0	36.0	36.0
40-44	34.82160544162216	36.0	36.0	36.0	34.4	36.0
45-49	34.724094535274055	36.0	36.0	36.0	32.0	36.0
50-54	34.61253856294424	36.0	36.0	36.0	32.0	36.0
55-59	34.50045052391242	36.0	36.0	36.0	32.0	36.0
60-64	34.382878732281426	36.0	36.0	36.0	32.0	36.0
65-69	34.20175860616192	36.0	36.0	36.0	32.0	36.0
70-74	34.08003696606461	36.0	36.0	36.0	32.0	36.0
75-79	33.95077329745451	36.0	36.0	36.0	32.0	36.0
80-84	33.82716054416221	36.0	36.0	36.0	30.0	36.0
85-89	33.64562794743209	36.0	36.0	36.0	27.0	36.0
90-94	33.44718337614329	36.0	36.0	36.0	27.0	36.0
95-99	33.31136094915807	36.0	36.0	36.0	27.0	36.0
100-104	33.1848119759177	36.0	36.0	36.0	22.2	36.0
105-109	33.07839184028486	36.0	36.0	36.0	21.0	36.0
110-114	33.00166958861662	36.0	36.0	36.0	18.2	36.0
115-119	32.881364074965006	36.0	36.0	36.0	14.0	36.0
120-124	32.7572892458651	36.0	32.0	36.0	14.0	36.0
125-129	32.64011837294954	36.0	32.0	36.0	14.0	36.0
130-134	32.5051494271619	36.0	32.0	36.0	14.0	36.0
135-139	32.33338293853033	36.0	32.0	36.0	14.0	36.0
140-144	32.160408257566495	36.0	32.0	36.0	14.0	36.0
145-149	31.935458202525105	36.0	32.0	36.0	14.0	36.0
150-151	30.34557324581074	34.0	29.5	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	4.0
16	11.0
17	36.0
18	94.0
19	198.0
20	398.0
21	772.0
22	1428.0
23	2318.0
24	3159.0
25	3935.0
26	4593.0
27	5142.0
28	5932.0
29	6837.0
30	7861.0
31	9759.0
32	13332.0
33	21241.0
34	51493.0
35	155779.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.66382808093028	32.358178321179295	20.231549759071356	21.746443838819076
2	19.189801680466697	36.808200514400845	27.07637527478315	16.92562253034931
3	17.56681457723174	27.768566400065236	35.369425323285384	19.295193699417645
4	18.058264302751486	28.283058230325437	28.740732686858255	24.91794478006483
5	19.398887609107064	30.77897941363341	29.9968401846963	19.82529279256322
6	20.798657011102087	30.778641311181264	28.848774420683114	19.57392725703353
7	21.51487557252946	30.061024504940402	29.01518137461435	19.40891854791579
8	19.89209134182514	29.51417320426878	30.212045949518046	20.38168950438804
9	20.344724485427527	29.266245812409537	30.27058799546075	20.118441706702185
10-14	20.745186884515128	30.23625921103775	29.391330819976027	19.627223084471094
15-19	20.754814422201385	29.864027398377296	29.404194017477337	19.97696416194398
20-24	20.75997879887471	29.59242195675514	29.437286799581415	20.210312444788737
25-29	20.9036979655074	29.35873391228714	29.525624821625147	20.211943300580312
30-34	21.149682727577897	29.090734884201343	29.37878752872712	20.38079485949364
35-39	21.031862833401423	29.627343590147966	29.174141298237288	20.16665227821332
40-44	21.05374748224319	29.44627018622566	28.95026190107288	20.549720430458265
45-49	21.04940205311293	29.16828336682253	29.275987874578952	20.50632670548559
50-54	21.235136865195155	29.22239422014496	28.763911785451697	20.778557129208192
55-59	21.369243993843497	29.297200694475116	28.980541785725205	20.353013525956175
60-64	21.33933036879186	29.495466208576147	28.77911085500565	20.38609256762634
65-69	21.25521277644284	29.55473457748896	28.80892094253116	20.381131703537044
70-74	21.215360506254104	29.694654621022977	28.509616626141508	20.58036824658141
75-79	21.66283635296148	29.19188308731892	28.71267482743202	20.432605732287573
80-84	21.56447340786702	29.458478699854073	28.54893116950467	20.428116722774238
85-89	21.39204414120181	29.533705527711984	28.537999450682435	20.536250880403774
90-94	21.45993611648914	29.360097219360366	28.61320281892622	20.566763845224276
95-99	21.39925053504075	29.274275349510106	28.701231069619205	20.625243045829944
100-104	21.61035012370625	29.397283017739046	28.5665916293123	20.425775229242404
105-109	21.520386650522237	29.256320497331885	28.81679039002943	20.40650246211645
110-114	21.70881026071804	29.3847076046046	28.50085428959398	20.405627845083384
115-119	21.691752570314147	29.31578339369806	28.50422326567501	20.48824077031279
120-124	21.66715486617104	29.33859274850431	28.650129503130707	20.34412288219395
125-129	21.975750680181257	29.333874351848266	28.212960112118612	20.47741485585187
130-134	22.06564442568773	29.179944178384513	28.31312569105923	20.441285704868523
135-139	22.44471170679341	29.10221833037479	27.90681231180497	20.546257651026835
140-144	22.46895419060645	29.357493820748687	27.72383301202133	20.449718976623533
145-149	22.507781755988333	29.361641092294104	27.615156053183092	20.515421098534468
150-151	22.902002236414123	28.775478296926458	27.782857104013647	20.539662362645768
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	47.410271919749356
2	36.11542421081601
3	36.862008276649384
4	42.97620908281631
5	39.22418040167028
6	40.37258426813562
7	40.92379412044525
8	40.27378084621318
9	40.463166192129705
10-14	40.372409968986226
15-19	40.73177858414536
20-24	40.970291243663446
25-29	41.11564126608771
30-34	41.53047758707154
35-39	41.198515111614746
40-44	41.603467912701454
45-49	41.555728758598526
50-54	42.01369399440335
55-59	41.722257519799676
60-64	41.72542293641819
65-69	41.63634447997988
70-74	41.795728752835515
75-79	42.095442085249054
80-84	41.992590130641254
85-89	41.928295021605585
90-94	42.02669996171341
95-99	42.0244935808707
100-104	42.03612535294866
105-109	41.92688911263868
110-114	42.11443810580142
115-119	42.17999334062693
120-124	42.01127774836498
125-129	42.45316553603312
130-134	42.506930130556256
135-139	42.99096935782025
140-144	42.91867316722998
145-149	43.0232028545228
150-151	43.44166459905989
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	10.5
2	11.5
3	14.0
4	23.0
5	31.0
6	36.0
7	42.5
8	62.0
9	81.5
10	87.5
11	100.5
12	127.0
13	160.5
14	215.0
15	288.0
16	368.5
17	486.5
18	643.0
19	814.0
20	1050.5
21	1383.0
22	1756.0
23	2166.5
24	2648.0
25	3185.0
26	3864.0
27	4533.0
28	5251.5
29	6104.0
30	7047.5
31	8052.5
32	9065.0
33	9795.0
34	9907.0
35	10070.0
36	10553.5
37	10990.5
38	11422.5
39	11671.5
40	11413.5
41	10981.0
42	10634.0
43	10403.0
44	9973.5
45	9517.5
46	8989.5
47	8313.5
48	7791.0
49	7099.5
50	6318.5
51	5591.5
52	4893.0
53	4318.5
54	3783.0
55	3291.5
56	2921.0
57	2537.0
58	2297.0
59	2203.5
60	2204.0
61	2305.5
62	2577.0
63	2789.5
64	2850.0
65	2808.0
66	2503.5
67	2114.5
68	1629.5
69	1146.5
70	825.0
71	554.5
72	357.5
73	269.0
74	214.0
75	157.5
76	108.5
77	80.5
78	71.5
79	54.0
80	39.5
81	38.5
82	30.0
83	27.0
84	22.0
85	14.5
86	14.5
87	11.0
88	6.0
89	4.5
90	1.5
91	3.0
92	7.0
93	5.0
94	5.0
95	12.5
96	19.0
97	27.0
98	59.0
99	242.5
100	460.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015629034669276037
2	3.3976162324513123E-4
3	6.795232464902625E-4
4	0.003397616232451312
5	0.0010192848697353936
6	0.018686889278482218
7	0.0054361859719221
8	3.3976162324513123E-4
9	6.795232464902625E-4
10-14	0.0010872371943844198
15-19	0.0
20-24	0.0
25-29	0.0
30-34	9.513325450863674E-4
35-39	0.03621858903793099
40-44	0.004892567374729889
45-49	6.795232464902624E-5
50-54	0.0017667604408746823
55-59	3.3976162324513123E-4
60-64	2.7180929859610495E-4
65-69	0.032277354208287465
70-74	0.007202946412796781
75-79	0.0017667604408746823
80-84	0.023035838056019897
85-89	0.047838436552914476
90-94	0.07685407917804868
95-99	0.047838436552914476
100-104	0.054158002745273916
105-109	0.049673149318438185
110-114	0.015085416072083826
115-119	6.795232464902625E-4
120-124	0.015425177695328958
125-129	0.021812696212337425
130-134	0.009988991723406858
135-139	0.0502167679156304
140-144	0.038596920400646904
145-149	0.03744173088161346
150-151	0.03499544719424852
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	294324.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	7.2470367192779825
#Duplication Level	Relative count
1	100.0
2	2.8023377791098456
3	0.23512146219711963
4	0.09611558674431704
5	0.050641545703995
6	0.03152177844840505
7	0.0253207728519975
8	0.019119767255589948
9	0.019636517721957243
10++	0.07027806342595225
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	522	0.17735556733395852	No Hit
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC	350	0.11891656813579593	No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	333	0.11314062054062869	No Hit
GATCCAGCCATAAAATGCATCATTCTTTTTTGTTTTAGACAACATTTCAT	314	0.1066851496989712	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	258235	13.3426485	27.289558	145-147
GATCG	111835	3.2728577	6.946312	1
GCCCC	51625	3.2360926	4.098945	9
GGGGC	56200	3.047521	4.17873	140-144
GGCCC	50555	3.0195446	3.5998313	4
CCTGG	65295	2.767748	5.1095357	4
CCAGC	59305	2.56658	11.069646	4
TCCAG	69935	2.1479661	9.278083	3
CAGCC	43080	1.8644005	6.488441	5
GATCC	54845	1.6844956	22.028505	1
GATCT	74055	1.6141968	21.736237	1
ATCTG	65635	1.4306638	11.229665	2
TCTGG	44595	1.3415353	6.9648232	3
ATCCA	59530	1.324814	9.322675	2
GATCA	60430	1.2814101	18.68345	1
ATCAG	56470	1.1974388	5.984301	2
CTGGT	37950	1.1416361	5.5498414	4
ATCAA	64890	0.9970099	5.2620416	2
>>END_MODULE