FastQCFastQC Report
Wed 14 Mar 2018
NAN20_S3_R2_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename NAN20_S3_R2_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 1020808
Filtered Sequences 0
Sequence length 151
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1328 0.13009302434933895 No Hit
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC 1096 0.107365929734093 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 847905 12.516361 34.188633 145-147
GCCCC 172465 3.2935858 4.9074664 9
GGGGC 207390 3.2649608 4.2967196 140-144
GATCG 382935 3.2407503 6.783674 1
AAAAA 1094085 3.0982485 4.087199 145-147
CCCTG 230145 3.0821497 4.258459 1
GGCCC 170600 3.0548346 4.1549315 8
CCAGC 198510 2.5549242 11.60642 4
TCCAG 216740 1.9562253 9.630263 3
CAGCC 143475 1.8465956 7.144164 5
GATCT 287360 1.8188223 19.241129 1
GATCA 258885 1.5747596 18.522919 1
ATCTG 215775 1.3657308 9.626123 2
AGCCA 152525 1.3230132 5.0577145 6
TCTGG 149495 1.3164482 6.3738976 3
ATCCA 195040 1.2652909 9.775648 2
GATCC 134250 1.2116971 21.485048 1
ATCAG 185285 1.1270616 6.4194264 2
CTGGT 127370 1.1216161 5.5419455 4
Produced by FastQC (version 0.10.1)