FastQCFastQC Report
Wed 14 Mar 2018
NAN20_S3_R1_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename NAN20_S3_R1_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 1020808
Filtered Sequences 0
Sequence length 151
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1847 0.1809351023894797 No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1477 0.14468930494275123 No Hit
GATCTGGTTTTATTACATTCTGAATTGGACGTTGAAAATGAGCTTATCTC 1070 0.1048189277513499 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 1049670 15.031044 32.73486 145-147
GATCG 425950 3.5611732 6.3403797 1
GCCCC 189710 3.4395192 4.3161464 9
GGGGC 214485 3.258 4.450054 140-144
GGCCC 187995 3.213187 3.9818816 4
CCCTG 245040 3.1514792 4.498684 1
GGGCC 192720 3.1052651 3.3652709 60-64
CCAGG 255110 3.0067134 3.6802917 8
CCAGC 206310 2.5793045 10.814294 4
TCCAG 246485 2.1859627 9.059679 3
CAGCC 147015 1.8379933 6.964515 5
GATCC 194440 1.7243994 20.646122 1
GATCT 250315 1.5747408 18.297808 1
ATCTG 226795 1.4267756 9.389806 2
ATCCA 207305 1.3447906 9.172219 2
TCTGG 152780 1.3140023 6.0680146 3
GATCA 213215 1.3039017 17.872437 1
ATCAG 197020 1.2048622 6.264903 2
Produced by FastQC (version 0.10.1)