FastQCFastQC Report
Tue 29 Jun 2021
AGal_1_S218_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAGal_1_S218_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35892651
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC8145292.2693475608697726TruSeq Adapter, Index 2 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2583410.7197601536871713TruSeq Adapter, Index 2 (100% over 50bp)
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA704240.1962072960283708No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT684210.1906267664653692No Hit
TGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGT649240.18088382493675376No Hit
TGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTA465670.12973965060424208No Hit
TCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTATTCTATGTTAT440190.12264070436034384No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG439000.12230916016763432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)