With this service you can check compatibility of adapters, i.e. find out whether there are pairs among entered adapters, whose indices differ by less than 3 bases (this is the check mode). In the addition mode you can find out what adapters are compatible with those you entered. |
Currently the service database includes adapters from Truseq v1/v2/LT, Truseq HT, Truseq Small RNA, Nextera, NEBNext Multiplex Oligos for Illumina (single index sets 1-4 and dual index sets 1 and 2), Ovation RRBS Methyl-Seq System 1-16 kits, NEXTflex DNA-Seq, NEBNext E6609, NEXTflex Rapid Directional qRNA-Seq, Lucigen NxSeq AmpFREE Low DNA.
In the input form adapters from Truseq v1/v2/LT and single-index NEB are to be written as ADnumber, Truseq HT as Dnumber, Truseq Small RNA as RPInumber, Nextera as Nnumber or Snumber, dual-index NEB as inumber, Ovation RRBS as BCnumber, NEXTflex DNA-Seq as NFnumber, NEBNext E6609 as Pnumber, NEXTflex Rapid Directional qRNA-Seq as NRnumber, Lucigen NxSeq AmpFREE Low DNA as NXnumber. A list with all adapters which the service knows can be found here
Alternatively, you can write not an adapter name, but an index sequence itself. Just keep in mind that the length of an index sequence you enter sould be the same as the length that you have selected above the entry field. Example of an entry:
For error reports and suggestions please write to firstname.lastname@example.org . The logo was made with the help of one of Duran pictures (vk.com/by_duran). The Russian version of ЧЕК can be found here.
|30 October 2018||Added adapters of Lucigen NxSeq AmpFREE Low DNA Library Kit.|
|8 August 2018||Index AD40 (kit E7730) changed in accordance with the new version of the kit.|
|25 July 2018||Added NEBNext Multiplex Oligos for Illumina, set 2 (kit e7780).|
|18 January 2018||Added the sequence that is read during index sequencing from the TruSeq Universal Adapter.|
|4 December 2017||Added adapters of "NEBNext E6609" and of "NEXTflex Rapid Directional qRNA-Seq". Also added an ability to enter not an adapter name, but, alternatively, its index sequence directly.|
|12 October 2017||Added single-index adapters of "NEXTflex DNA-Seq".|
|17 March 2017||Added single-index adapters of "NEBNext Multiplex Oligos for Illumina", sets 3 and 4. They are named AD17, AD24, AD26 and AD28-48. Adapters of "NEBNext Multiplex Oligos for Illumina", sets 1 and 2, have the same indices as adapters of Truseq v1/v2/LT, and therefore were not added.|
|10 October 2016||Added the list of updates|
|2 September 2016||Added adapters of Nextera v1 kit that are absent in Nextera v2 (N708, N709, S501, S504)|